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alpha-Tubulin antibody - 302 308

alpha-Tubulin is a major cytoskeleton protein
Monoclonal Recombinant Guinea pig recombinant IgG
Cat. No.: 302 308
Amount: 50 µg
Price: $415.00
Cat. No. 302 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 1000 gallery  
Clone Gp3A2
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity Reacts with: human (P68366), rat (Q5XIF6), mouse (P68368), vertebrates, invertebrates, yeast.
Other species not tested yet.
Specificity Specific for α-tubulin (glu- and tyr-α-tubulin)
Matching control protein/peptide 302-21P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 3A2. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 302_308.pdf
Cat. No.: 302 308
Quantity: 50 µg
Price: $415.00
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric α-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated α-tubulin is referred to as Glu-α-tubulin. Another post-translational modification of detyrosinated α-tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons.