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alpha-Tubulin - 302 211C3

A major cytoskeleton protein
Monoclonal mouse purified IgG
Cat. No.: 302 211C3
Amount: 50 µg
Price: $465.00
Cat. No. 302 211C3 50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label Oyster 550
Clone 3A2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Epitop Epitop: AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast.
Other species not tested yet.
Specificity Specific for α-tubulin (glu- and tyr-α-tubulin).
Matching control protein/peptide 302-21P
Data sheet 302_211c3.pdf
Cat. No.: 302 211C3
Quantity: 50 µg
Price: $465.00
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric α-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated α-tubulin is referred to as Glu-α-tubulin. Another post-translational modification of detyrosinated α-tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons.

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