Cat. No.: 302 211C3
Amount: 50 µg
Price:
$465.00
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| Cat. No. 302 211C3 |
50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster 550
Fluorescence labeled antibodies conjugated to Oyster dyes from
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes! |
| Applications |
IP: N/A ICC: 1 : 500 up to 1 : 1000 gallery IHC: not tested yet IHC-P: not tested yet |
| Label | Oyster 550 |
| Clone | 3A2 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366) |
| Reactivity |
Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast. Other species not tested yet. |
| Specificity | Specific for α-tubulin (glu- and tyr-α-tubulin). |
| Matching control protein/peptide | 302-21P |
| Data sheet | 302_211c3.pdf |
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Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.
Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.