|Cat. No. 302 211C3||
50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550
Fluorescence labeled antibodies conjugated to Oyster dyes from
Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.
CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)|
Epitop: AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast.
Other species not tested yet.
|Specificity||Specific for α-tubulin (glu- and tyr-α-tubulin).|
|Matching control protein/peptide||302-21P|
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.
Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.