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alpha-Tubulin antibody - 302 211C3

alpha-Tubulin is a major cytoskeleton protein
Mouse monoclonal purified IgG
Cat. No.: 302 211C3
Amount: 50 µg
Price: $465.00
Cat. No. 302 211C3 50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster  550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established fluorescence detection systems.

Oyster 550: λex 551 nm / λem 570 nm
Oyster 650: λex 651 nm / λem 671 nm

Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.
Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label Oyster 550
Clone 3A2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast.
Other species not tested yet.
Specificity Specific for α-tubulin (glu- and tyr-α-tubulin).
Matching control protein/peptide 302-21P
Data sheet 302_211c3.pdf
Cat. No.: 302 211C3
Amount: 50 µg
Price: $465.00
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.

Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.