Synaptic Systems - alpha-Tubulin

Cat. No.: 302 209

Amount: 50 µg

Price: $420.00

WB

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.

Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.

1 ) Erck C et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
2 ) Contin MA et al., Biochem. J. (1999)
3 ) Paturle-Lafanechère L et al., J. Cell. Sci. (1994)
4 ) Ersfeld K et al., J. Cell Biol. (1993)
5 ) Rüdiger M et al., FEBS Lett. (1992)
6 ) Caron JM et al., J. Cell Biol. (1985)
7 ) Caron JM et al., Nature ()

Protocols

Other products for this target

Cat. No. 302 209	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP-staining) gallery ICC: 1 : 1000 up to 1 : 2000 (see remarks) gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery
Clone	Ch3A2
Subtype	IgY (λ light chain)
Immunogen	Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity	Reacts with: human (P68366), rat (Q5XIF6), mouse (P68368), vertebrates, invertebrates, yeast. Other species not tested yet.
Specificity	Specific for α-tubulin (glu- and tyr-α-tubulin)
Matching control protein/peptide	302-21P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 3A2. The constant regions of the heavy and light chains have been replaced with chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells. ICC: Methanol or methanol-acetone fixation is possible.
Data sheet	302_209.pdf

alpha-Tubulin antibody - 302 209