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c-Fos - 226 005

A member of the transcription factor complex AP1
Polyclonal Guinea pig purified antibody
Cat. No.: 226 005
Amount: 50 µg
Price: $460.00
Cat. No. 226 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery  
IHC-P/FFPE: 1 : 500 up to 1 : 1000 (see remarks) gallery  
Immunogen Synthetic peptide corresponding to AA 2 to 17 from rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: rat (P12841), mouse (P01101).
Other species not tested yet.
Specificity Specific for c-Fos.
Matching control protein/peptide 226-0P
Remarks

IHC: Signal quality is strongly enhanced when antibody is incubated at RT.
For best results tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.
IHC-P: Signal quality is strongly enhanced when antibody is incubated at RT.

Data sheet 226_005.pdf

References for c-Fos - 226 005

Activity-Dependent Plasticity of Axo-axonic Synapses at the Axon Initial Segment.
Pan-Vazquez A, Wefelmeyer W, Gonzalez Sabater V, Neves G, Burrone J
Neuron (2020) : . 226 005 IHC; tested species: mouse
A brainstem-central amygdala circuit underlies defensive responses to learned threats.
Gu Y, Piper WT, Branigan LA, Vazey EM, Aston-Jones G, Lin L, LeDoux JE, Sears RM
Molecular psychiatry (2020) 253: 640-654. 226 005 IHC; tested species: rat
Treatment with Mesenchymal-Derived Extracellular Vesicles Reduces Injury-Related Pathology in Pyramidal Neurons of Monkey Perilesional Ventral Premotor Cortex.
Medalla M, Chang W, Calderazzo SM, Go V, Tsolias A, Goodliffe JW, Pathak D, De Alba D, Pessina M, Rosene DL, Buller B, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4017: 3385-3407. 226 005 IHC; tested species: monkey
Cat. No.: 226 005
Quantity: 50 µg
Price: $460.00
Activity-Dependent Plasticity of Axo-axonic Synapses at the Axon Initial Segment.
Pan-Vazquez A, Wefelmeyer W, Gonzalez Sabater V, Neves G, Burrone J
Neuron (2020) : . 226 005 IHC; tested species: mouse
A brainstem-central amygdala circuit underlies defensive responses to learned threats.
Gu Y, Piper WT, Branigan LA, Vazey EM, Aston-Jones G, Lin L, LeDoux JE, Sears RM
Molecular psychiatry (2020) 253: 640-654. 226 005 IHC; tested species: rat
Treatment with Mesenchymal-Derived Extracellular Vesicles Reduces Injury-Related Pathology in Pyramidal Neurons of Monkey Perilesional Ventral Premotor Cortex.
Medalla M, Chang W, Calderazzo SM, Go V, Tsolias A, Goodliffe JW, Pathak D, De Alba D, Pessina M, Rosene DL, Buller B, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4017: 3385-3407. 226 005 IHC; tested species: monkey
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).