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c-Fos - 226 004

A member of the transcription factor complex AP1
Polyclonal Guinea pig antiserum
Cat. No.: 226 004
Amount: 100 µl
Price: 370.00 €
Cat. No. 226 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: 1 : 1000 up to 1 : 2000 (see remarks) gallery  
FACS: yes
Immunogen Synthetic peptide corresponding to AA 2 to 17 from rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: human (P01100), rat (P12841), mouse (P01101), monkey, ape, cow, dog, pig.
Other species not tested yet.
Specificity Specific for c-Fos.
Matching control protein/peptide 226-0P
Remarks

IHC: Signal quality is strongly enhanced when antibody is incubated at RT.
For best results tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.
IHC-P: Signal quality is strongly enhanced when antibody is incubated at RT.

Data sheet 226_004.pdf

References for c-Fos - 226 004

Chronic sleep disruption induces depression-like behavior in adolescent male and female mice and sensitization of the hypothalamic-pituitary-adrenal axis in adolescent female mice.
Murack M, Chandrasegaram R, Smith KB, Ah-Yen EG, Rheaume É, Malette-Guyon É, Nanji Z, Semchishen SN, Latus O, Messier C, Ismail N, et al.
Behavioural brain research (2021) 399: 113001. 226 004 IHC; tested species: mouse
Parabrachial neuron types categorically encode thermoregulation variables during heat defense.
Yang WZ, Du X, Zhang W, Gao C, Xie H, Xiao Y, Jia X, Liu J, Xu J, Fu X, Tu H, et al.
Science advances (2020) 636: . 226 004 IHC; tested species: mouse
Somatostatin neurons in the central amygdala mediate anxiety by disinhibition of the central sublenticular extended amygdala.
Sun Y, Qian L, Xu L, Hunt S, Sah P
Molecular psychiatry (2020) : . 226 004 IHC; tested species: mouse
VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression.
Zha X, Wang L, Jiao ZL, Yang RR, Xu C, Xu XH
Cell reports (2020) 313: 107517. 226 004 IHC; tested species: mouse
KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain.
Lipinski M, Muñoz-Viana R, Del Blanco B, Marquez-Galera A, Medrano-Relinque J, Caramés JM, Szczepankiewicz AA, Fernandez-Albert J, Navarrón CM, Olivares R, Wilczyński GM, et al.
Nature communications (2020) 111: 2588. 226 004 IHC; tested species: mouse
Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams.
Doucette E, Merfeld E, Leblanc H, Monasterio A, Cincotta C, Grella SL, Logan J, Ramirez S
Neurobiology of learning and memory (2020) 176: 107321. 226 004 IHC; tested species: mouse
SATB2-LEMD2 interaction links nuclear shape plasticity to regulation of cognition-related genes.
Feurle P, Abentung A, Cera I, Wahl N, Ablinger C, Bucher M, Stefan E, Sprenger S, Teis D, Fischer A, Laighneach A, et al.
The EMBO journal (2020) : e103701. 226 004 IHC; tested species: mouse
Parabrachial nucleus circuit governs neuropathic pain-like behavior.
Sun L, Liu R, Guo F, Wen MQ, Ma XL, Li KY, Sun H, Xu CL, Li YY, Wu MY, Zhu ZG, et al.
Nature communications (2020) 111: 5974. 226 004 IHC; tested species: mouse
Involvement of the Nucleus Accumbens in Chocolate-induced Cataplexy.
Su J, Li Z, Yamashita A, Kusumoto-Yoshida I, Isomichi T, Hao L, Kuwaki T
Scientific reports (2020) 101: 4958. 226 004 IHC; tested species: mouse
Tactile modulation of memory and anxiety requires dentate granule cells along the dorsoventral axis.
Wang C, Liu H, Li K, Wu ZZ, Wu C, Yu JY, Gong Q, Fang P, Wang XX, Duan SM, Wang H, et al.
Nature communications (2020) 111: 6045. 226 004 IHC; tested species: mouse
The gut-brain axis mediates sugar preference.
Tan HE, Sisti AC, Jin H, Vignovich M, Villavicencio M, Tsang KS, Goffer Y, Zuker CS
Nature (2020) 5807804: 511-516. 226 004 IHC; tested species: mouse
A Novel Cortical Mechanism for Top-Down Control of Water Intake.
Zhao Z, Soria-Gómez E, Varilh M, Covelo A, Julio-Kalajzić F, Cannich A, Castiglione A, Vanhoutte L, Duveau A, Zizzari P, Beyeler A, et al.
Current biology : CB (2020) : . 226 004 IHC; tested species: mouse
Corticotropin-Releasing Factor-Producing Cells in the Paraventricular Nucleus of the Hypothalamus and Extended Amygdala Show Age-Dependent FOS and FOSB/DeltaFOSB Immunoreactivity in Acute and Chronic Stress Models in the Rat.
Kovács LÁ, Berta G, Csernus V, Ujvári B, Füredi N, Gaszner B
Frontiers in aging neuroscience (2019) 11: 274. 226 004 IHC; tested species: rat
Considerations related to the use of short neuropeptide promoters in viral vectors targeting hypothalamic neurons.
Kakava-Georgiadou N, Bullich-Vilarrubias C, Zwartkruis MM, Luijendijk MCM, Garner KM, Adan RAH
Scientific reports (2019) 91: 11146. 226 004 IHC; tested species: rat
Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice.
Ito T, Tatsumi K, Takimoto Y, Nishimura T, Imai T, Yamanaka T, Takeda N, Wanaka A, Kitahara T
Brain sciences (2019) 911: . 226 004 IHC; tested species: mouse
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 226 004 IHC; tested species: mouse
Immediate and deferred epigenomic signatures of in vivo neuronal activation in mouse hippocampus.
Fernandez-Albert J, Lipinski M, Lopez-Cascales MT, Rowley MJ, Martin-Gonzalez AM, Del Blanco B, Corces VG, Barco A
Nature neuroscience (2019) : . 226 004 IHC; tested species: mouse
Phenotyping neurons activated in the mouse brain during restoration of salt debt.
Smith CM, Cross J, Eraslan IM, Attawar A, Ch'ng S, Dhar P, Samarasinghe R, Gray L, Lawrence AJ
Journal of chemical neuroanatomy (2019) : 101665. 226 004 IHC; tested species: rat
Transient, Consequential Increases in Extracellular Potassium Ions Accompany Channelrhodopsin2 Excitation.
Octeau JC, Gangwani MR, Allam SL, Tran D, Huang S, Hoang-Trong TM, Golshani P, Rumbell TH, Kozloski JR, Khakh BS
Cell reports (2019) 278: 2249-2261.e7. 226 004 IHC; tested species: mouse
Repetitive hypoglycaemia reduces the activation of glucose responsive neurons in the C1 and C3 medullary brain regions to subsequent hypoglycaemia.
Kakall ZM, Kavurma MM, Cohen EM, R Howe P, Nedoboy PE, Pilowsky PM
American journal of physiology. Endocrinology and metabolism (2019) : . 226 004 IHC; tested species: rat
Crucial role of feedback signals from prelimbic cortex to basolateral amygdala in the retrieval of morphine withdrawal memory.
Song J, Shao D, Guo X, Zhao Y, Cui D, Ma Q, Sheng H, Ma L, Lai B, Chen M, Zheng P, et al.
Science advances (2019) 52: eaat3210. 226 004 IHC; tested species: mouse
Structural and functional alterations in the prefrontal cortex after post-weaning social isolation: relationship with species-typical and deviant aggression.
Biro L, Toth M, Sipos E, Bruzsik B, Tulogdi A, Bendahan S, Sandi C, Haller J
Brain structure & function (2017) 2224: 1861-1875. 226 004 IHC; tested species: rat
Dynamic changes in the relationship of microglia to cardiovascular neurons in response to increases and decreases in blood pressure.
Kapoor K, Bhandare AM, Nedoboy PE, Mohammed S, Farnham MM, Pilowsky PM
Neuroscience (2016) 329: 12-29. 226 004 IHC
Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo.
McAvoy KM, Rajamohamed Sait H, Marsh G, Peterson M, Reynolds TL, Gagnon J, Geisler S, Leach P, Roberts C, Cahir-McFarland E, Ransohoff RM, et al.
PloS one (2019) 148: e0220125. 226 004 IHC-P; tested species: mouse
Cat. No.: 226 004
Quantity: 100 µl
Price: 370.00 €
Chronic sleep disruption induces depression-like behavior in adolescent male and female mice and sensitization of the hypothalamic-pituitary-adrenal axis in adolescent female mice.
Murack M, Chandrasegaram R, Smith KB, Ah-Yen EG, Rheaume É, Malette-Guyon É, Nanji Z, Semchishen SN, Latus O, Messier C, Ismail N, et al.
Behavioural brain research (2021) 399: 113001. 226 004 IHC; tested species: mouse
Parabrachial neuron types categorically encode thermoregulation variables during heat defense.
Yang WZ, Du X, Zhang W, Gao C, Xie H, Xiao Y, Jia X, Liu J, Xu J, Fu X, Tu H, et al.
Science advances (2020) 636: . 226 004 IHC; tested species: mouse
Somatostatin neurons in the central amygdala mediate anxiety by disinhibition of the central sublenticular extended amygdala.
Sun Y, Qian L, Xu L, Hunt S, Sah P
Molecular psychiatry (2020) : . 226 004 IHC; tested species: mouse
VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression.
Zha X, Wang L, Jiao ZL, Yang RR, Xu C, Xu XH
Cell reports (2020) 313: 107517. 226 004 IHC; tested species: mouse
KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain.
Lipinski M, Muñoz-Viana R, Del Blanco B, Marquez-Galera A, Medrano-Relinque J, Caramés JM, Szczepankiewicz AA, Fernandez-Albert J, Navarrón CM, Olivares R, Wilczyński GM, et al.
Nature communications (2020) 111: 2588. 226 004 IHC; tested species: mouse
Social behavior in mice following chronic optogenetic stimulation of hippocampal engrams.
Doucette E, Merfeld E, Leblanc H, Monasterio A, Cincotta C, Grella SL, Logan J, Ramirez S
Neurobiology of learning and memory (2020) 176: 107321. 226 004 IHC; tested species: mouse
SATB2-LEMD2 interaction links nuclear shape plasticity to regulation of cognition-related genes.
Feurle P, Abentung A, Cera I, Wahl N, Ablinger C, Bucher M, Stefan E, Sprenger S, Teis D, Fischer A, Laighneach A, et al.
The EMBO journal (2020) : e103701. 226 004 IHC; tested species: mouse
Parabrachial nucleus circuit governs neuropathic pain-like behavior.
Sun L, Liu R, Guo F, Wen MQ, Ma XL, Li KY, Sun H, Xu CL, Li YY, Wu MY, Zhu ZG, et al.
Nature communications (2020) 111: 5974. 226 004 IHC; tested species: mouse
Involvement of the Nucleus Accumbens in Chocolate-induced Cataplexy.
Su J, Li Z, Yamashita A, Kusumoto-Yoshida I, Isomichi T, Hao L, Kuwaki T
Scientific reports (2020) 101: 4958. 226 004 IHC; tested species: mouse
Tactile modulation of memory and anxiety requires dentate granule cells along the dorsoventral axis.
Wang C, Liu H, Li K, Wu ZZ, Wu C, Yu JY, Gong Q, Fang P, Wang XX, Duan SM, Wang H, et al.
Nature communications (2020) 111: 6045. 226 004 IHC; tested species: mouse
The gut-brain axis mediates sugar preference.
Tan HE, Sisti AC, Jin H, Vignovich M, Villavicencio M, Tsang KS, Goffer Y, Zuker CS
Nature (2020) 5807804: 511-516. 226 004 IHC; tested species: mouse
A Novel Cortical Mechanism for Top-Down Control of Water Intake.
Zhao Z, Soria-Gómez E, Varilh M, Covelo A, Julio-Kalajzić F, Cannich A, Castiglione A, Vanhoutte L, Duveau A, Zizzari P, Beyeler A, et al.
Current biology : CB (2020) : . 226 004 IHC; tested species: mouse
Corticotropin-Releasing Factor-Producing Cells in the Paraventricular Nucleus of the Hypothalamus and Extended Amygdala Show Age-Dependent FOS and FOSB/DeltaFOSB Immunoreactivity in Acute and Chronic Stress Models in the Rat.
Kovács LÁ, Berta G, Csernus V, Ujvári B, Füredi N, Gaszner B
Frontiers in aging neuroscience (2019) 11: 274. 226 004 IHC; tested species: rat
Considerations related to the use of short neuropeptide promoters in viral vectors targeting hypothalamic neurons.
Kakava-Georgiadou N, Bullich-Vilarrubias C, Zwartkruis MM, Luijendijk MCM, Garner KM, Adan RAH
Scientific reports (2019) 91: 11146. 226 004 IHC; tested species: rat
Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice.
Ito T, Tatsumi K, Takimoto Y, Nishimura T, Imai T, Yamanaka T, Takeda N, Wanaka A, Kitahara T
Brain sciences (2019) 911: . 226 004 IHC; tested species: mouse
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 226 004 IHC; tested species: mouse
Immediate and deferred epigenomic signatures of in vivo neuronal activation in mouse hippocampus.
Fernandez-Albert J, Lipinski M, Lopez-Cascales MT, Rowley MJ, Martin-Gonzalez AM, Del Blanco B, Corces VG, Barco A
Nature neuroscience (2019) : . 226 004 IHC; tested species: mouse
Phenotyping neurons activated in the mouse brain during restoration of salt debt.
Smith CM, Cross J, Eraslan IM, Attawar A, Ch'ng S, Dhar P, Samarasinghe R, Gray L, Lawrence AJ
Journal of chemical neuroanatomy (2019) : 101665. 226 004 IHC; tested species: rat
Transient, Consequential Increases in Extracellular Potassium Ions Accompany Channelrhodopsin2 Excitation.
Octeau JC, Gangwani MR, Allam SL, Tran D, Huang S, Hoang-Trong TM, Golshani P, Rumbell TH, Kozloski JR, Khakh BS
Cell reports (2019) 278: 2249-2261.e7. 226 004 IHC; tested species: mouse
Repetitive hypoglycaemia reduces the activation of glucose responsive neurons in the C1 and C3 medullary brain regions to subsequent hypoglycaemia.
Kakall ZM, Kavurma MM, Cohen EM, R Howe P, Nedoboy PE, Pilowsky PM
American journal of physiology. Endocrinology and metabolism (2019) : . 226 004 IHC; tested species: rat
Crucial role of feedback signals from prelimbic cortex to basolateral amygdala in the retrieval of morphine withdrawal memory.
Song J, Shao D, Guo X, Zhao Y, Cui D, Ma Q, Sheng H, Ma L, Lai B, Chen M, Zheng P, et al.
Science advances (2019) 52: eaat3210. 226 004 IHC; tested species: mouse
Structural and functional alterations in the prefrontal cortex after post-weaning social isolation: relationship with species-typical and deviant aggression.
Biro L, Toth M, Sipos E, Bruzsik B, Tulogdi A, Bendahan S, Sandi C, Haller J
Brain structure & function (2017) 2224: 1861-1875. 226 004 IHC; tested species: rat
Dynamic changes in the relationship of microglia to cardiovascular neurons in response to increases and decreases in blood pressure.
Kapoor K, Bhandare AM, Nedoboy PE, Mohammed S, Farnham MM, Pilowsky PM
Neuroscience (2016) 329: 12-29. 226 004 IHC
Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo.
McAvoy KM, Rajamohamed Sait H, Marsh G, Peterson M, Reynolds TL, Gagnon J, Geisler S, Leach P, Roberts C, Cahir-McFarland E, Ransohoff RM, et al.
PloS one (2019) 148: e0220125. 226 004 IHC-P; tested species: mouse
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).