|Cat. No. 198 011||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: not tested yet
ICC: 1 : 500 gallery
IHC: not recommended
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 3 to 101 from mouse GAD1 (UniProt Id: P48318)|
Epitop: AA 7 to 25 from mouse GAD1 (UniProt Id: P48318)
Reacts with: rat (P18088), mouse (P48318).
Other species not tested yet.
|Specificity||Specific for GAD 1 / GAD67.|
The glutamic acid decarboxylases GAD 1, also referred to as GAD 67, and GAD 2 / GAD 65 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.