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GAD 1 / GAD 67 antibody - 198 011

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Monoclonal mouse purified IgG
Cat. No.: 198 011
Amount: 100 µg
Price: $415.00
Cat. No. 198 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: not tested yet
IP: yes
ICC: 1 : 500 gallery  
IHC: not recommended
IHC-P/FFPE: not tested yet
Clone 120G4
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 101 from mouse GAD1 (UniProt Id: P48318)
Epitop Epitop: AA 7 to 25 from mouse GAD1 (UniProt Id: P48318)
Reactivity Reacts with: rat (P18088), mouse (P48318).
Other species not tested yet.
Specificity Specific for GAD 1 / GAD67.
Data sheet 198_011.pdf

References for GAD 1 / GAD 67 - 198 011

Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 198 011 ICC
Cat. No.: 198 011
Quantity: 100 µg
Price: $415.00
Cellular distribution of the NMDA-receptor activated synapto-nuclear messenger Jacob in the rat brain.
Mikhaylova M, Karpova A, Bär J, Bethge P, YuanXiang P, Chen Y, Zuschratter W, Behnisch T, Kreutz MR
Brain structure & function (2014) 2193: 843-60. 198 011 ICC
Background

The glutamic acid decarboxylases GAD 1, also referred to as GAD 67, and GAD 2 / GAD 65 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.