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GAD 1 / GAD 67 antibody - 198 006

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Polyclonal chicken purified antibody
Cat. No.: 198 006
Amount: 50 µg
Price: $370.00
Cat. No. 198 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 3 to 101 from mouse GAD1 (UniProt Id: P48318)
Reactivity Reacts with: rat (P18088), mouse (P48318).
Other species not tested yet.
Specificity Specific for GAD 1 / GAD 67.
Data sheet 198_006.pdf

References for GAD 1 / GAD 67 - 198 006

Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.
Yang Q, Huang Z, Luo Y, Zheng F, Hu Y, Liu H, Zhu S, He M, Xu D, Li Y, Yang M, et al.
EBioMedicine (2019) : . 198 006 IHC; tested species: mouse
NETO1 Regulates Postsynaptic Kainate Receptors in CA3 Interneurons During Circuit Maturation.
Orav E, Dowavic I, Huupponen J, Taira T, Lauri SE
Molecular neurobiology (2019) : . 198 006 IHC; tested species: mouse
ZDHHC8 critically regulates seizure susceptibility in epilepsy.
Yang Q, Zheng F, Hu Y, Yang Y, Li Y, Chen G, Wang W, He M, Zhou R, Ma Y, Xu D, et al.
Cell death & disease (2018) 98: 795. 198 006 IHC; tested species: human,mouse
Cat. No.: 198 006
Quantity: 50 µg
Price: $370.00
Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.
Yang Q, Huang Z, Luo Y, Zheng F, Hu Y, Liu H, Zhu S, He M, Xu D, Li Y, Yang M, et al.
EBioMedicine (2019) : . 198 006 IHC; tested species: mouse
NETO1 Regulates Postsynaptic Kainate Receptors in CA3 Interneurons During Circuit Maturation.
Orav E, Dowavic I, Huupponen J, Taira T, Lauri SE
Molecular neurobiology (2019) : . 198 006 IHC; tested species: mouse
ZDHHC8 critically regulates seizure susceptibility in epilepsy.
Yang Q, Zheng F, Hu Y, Yang Y, Li Y, Chen G, Wang W, He M, Zhou R, Ma Y, Xu D, et al.
Cell death & disease (2018) 98: 795. 198 006 IHC; tested species: human,mouse
Background

The glutamic acid decarboxylases GAD 1, also referred to as GAD 67, and GAD 2 / GAD 65 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.