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Bassoon antibody - 141 002

Bassoon is a large multi-domain protein of the presynaptic active zone
Polyclonal rabbit antiserum
Cat. No.: 141 002
Amount: 200 µl
Price: $350.00
Cat. No. 141 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 2000 gallery  
IHC: yes
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
No signal: chicken.
Other species not tested yet.
Specificity Specific for bassoon.
Matching control protein/peptide 141-0P
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 141_002.pdf

References for Bassoon - 141 002

No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 141 002 WB, ICC, IHC; tested species: human,mouse
Critical role for piccolo in synaptic vesicle retrieval.
Ackermann F, Schink KO, Bruns C, Izsvák Z, Hamra FK, Rosenmund C, Garner CC
eLife (2019) 8: . 141 002 WB, ICC; tested species: rat
Distribution of serine/threonine kinase SAD-B in mouse peripheral nerve synapse.
Hagiwara A, Harada K, Hida Y, Kitajima I, Ohtsuka T
Neuroreport (2011) 227: 319-25. 141 002 WB; tested species: mouse
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 141 002 ICC; tested species: rat
Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms.
Koch N, Koch D, Krueger S, Tröger J, Sabanov V, Ahmed T, McMillan LE, Wolf D, Montag D, Kessels MM, Balschun D, et al.
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 141 002 ICC; tested species: mouse
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 141 002 WB, ICC, IHC; tested species: human,mouse
Sonic hedgehog expression in the postnatal brain.
Rivell A, Petralia RS, Wang YX, Clawson E, Moehl K, Mattson MP, Yao PJ
Biology open (2019) : . 141 002 ICC; tested species: rat
Critical role for piccolo in synaptic vesicle retrieval.
Ackermann F, Schink KO, Bruns C, Izsvák Z, Hamra FK, Rosenmund C, Garner CC
eLife (2019) 8: . 141 002 WB, ICC; tested species: rat
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons.
Nazir FH, Becker B, Brinkmalm A, Höglund K, Sandelius Å, Bergström P, Satir TM, Öhrfelt A, Blennow K, Agholme L, Zetterberg H, et al.
Neurochemistry international (2018) : . 141 002 ICC; tested species: mouse
Astrocyte-Secreted Chordin-like 1 Drives Synapse Maturation and Limits Plasticity by Increasing Synaptic GluA2 AMPA Receptors.
Blanco-Suarez E, Liu TF, Kopelevich A, Allen NJ
Neuron (2018) : . 141 002 ICC; tested species: mouse
Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 141 002 ICC; tested species: mouse
Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation.
Farhy-Tselnicker I, van Casteren ACM, Lee A, Chang VT, Aricescu AR, Allen NJ
Neuron (2017) 962: 428-445.e13. 141 002 ICC; tested species: rat
Correlated optical and isotopic nanoscopy.
Saka SK, Vogts A, Kröhnert K, Hillion F, Rizzoli SO, Wessels JT
Nature communications (2014) 5: 3664. 141 002 ICC; tested species: rat
A local, periactive zone endocytic machinery at photoreceptor synapses in close vicinity to synaptic ribbons.
Wahl S, Katiyar R, Schmitz F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3325: 10278-300. 141 002 ICC
Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves.
Juranek JK, Mukherjee K, Jahn R, Li JY
Biochemical and biophysical research communications (2021) 559: 92-98. 141 002 IHC; tested species: rat
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 141 002 WB, ICC, IHC; tested species: human,mouse
Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio.
Park HE, Choi D, Park JS, Sim C, Park S, Kang S, Yim H, Lee M, Kim J, Pac J, Rhee K, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2019) 622: 1901673. 141 002 IHC; tested species: mouse
Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice.
Nishimune H, Badawi Y, Mori S, Shigemoto K
Scientific reports (2016) 6: 27935. 141 002 IHC; tested species: mouse
Photoreceptor calcium channels: insight from night blindness.
Morgans CW, Bayley PR, Oesch NW, Ren G, Akileswaran L, Taylor WR
Visual neuroscience () 225: 561-8. 141 002 IHC; tested species: mouse
Cat. No.: 141 002
Quantity: 200 µl
Price: $350.00
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 141 002 WB, ICC, IHC; tested species: human,mouse
Critical role for piccolo in synaptic vesicle retrieval.
Ackermann F, Schink KO, Bruns C, Izsvák Z, Hamra FK, Rosenmund C, Garner CC
eLife (2019) 8: . 141 002 WB, ICC; tested species: rat
Distribution of serine/threonine kinase SAD-B in mouse peripheral nerve synapse.
Hagiwara A, Harada K, Hida Y, Kitajima I, Ohtsuka T
Neuroreport (2011) 227: 319-25. 141 002 WB; tested species: mouse
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 141 002 ICC; tested species: rat
Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms.
Koch N, Koch D, Krueger S, Tröger J, Sabanov V, Ahmed T, McMillan LE, Wolf D, Montag D, Kessels MM, Balschun D, et al.
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 141 002 ICC; tested species: mouse
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 141 002 WB, ICC, IHC; tested species: human,mouse
Sonic hedgehog expression in the postnatal brain.
Rivell A, Petralia RS, Wang YX, Clawson E, Moehl K, Mattson MP, Yao PJ
Biology open (2019) : . 141 002 ICC; tested species: rat
Critical role for piccolo in synaptic vesicle retrieval.
Ackermann F, Schink KO, Bruns C, Izsvák Z, Hamra FK, Rosenmund C, Garner CC
eLife (2019) 8: . 141 002 WB, ICC; tested species: rat
Expression and secretion of synaptic proteins during stem cell differentiation to cortical neurons.
Nazir FH, Becker B, Brinkmalm A, Höglund K, Sandelius Å, Bergström P, Satir TM, Öhrfelt A, Blennow K, Agholme L, Zetterberg H, et al.
Neurochemistry international (2018) : . 141 002 ICC; tested species: mouse
Astrocyte-Secreted Chordin-like 1 Drives Synapse Maturation and Limits Plasticity by Increasing Synaptic GluA2 AMPA Receptors.
Blanco-Suarez E, Liu TF, Kopelevich A, Allen NJ
Neuron (2018) : . 141 002 ICC; tested species: mouse
Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 141 002 ICC; tested species: mouse
Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation.
Farhy-Tselnicker I, van Casteren ACM, Lee A, Chang VT, Aricescu AR, Allen NJ
Neuron (2017) 962: 428-445.e13. 141 002 ICC; tested species: rat
Correlated optical and isotopic nanoscopy.
Saka SK, Vogts A, Kröhnert K, Hillion F, Rizzoli SO, Wessels JT
Nature communications (2014) 5: 3664. 141 002 ICC; tested species: rat
A local, periactive zone endocytic machinery at photoreceptor synapses in close vicinity to synaptic ribbons.
Wahl S, Katiyar R, Schmitz F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3325: 10278-300. 141 002 ICC
Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves.
Juranek JK, Mukherjee K, Jahn R, Li JY
Biochemical and biophysical research communications (2021) 559: 92-98. 141 002 IHC; tested species: rat
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 141 002 WB, ICC, IHC; tested species: human,mouse
Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio.
Park HE, Choi D, Park JS, Sim C, Park S, Kang S, Yim H, Lee M, Kim J, Pac J, Rhee K, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2019) 622: 1901673. 141 002 IHC; tested species: mouse
Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice.
Nishimune H, Badawi Y, Mori S, Shigemoto K
Scientific reports (2016) 6: 27935. 141 002 IHC; tested species: mouse
Photoreceptor calcium channels: insight from night blindness.
Morgans CW, Bayley PR, Oesch NW, Ren G, Akileswaran L, Taylor WR
Visual neuroscience () 225: 561-8. 141 002 IHC; tested species: mouse
Background

Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.