Synaptic Systems - Synaptobrevin2

Cat. No.: 104-2P

Amount: 100 µg

Price: $110.00

Background

Synaptobrevins, also known as vesicle-associated membrane proteins (VAMPs), are predominantly expressed in the nervous system and are classified within the brevin subfamily of the SNARE (Soluble NSF Attachment Protein Receptor) protein superfamily. Brevins are small integral transmembrane proteins characterized by a central SNARE motif, an N-terminal cytoplasmic domain, and a C-terminal transmembrane domain. As crucial components of the SNARE machinery, these proteins play an essential role in vesicular transport and membrane fusion processes within cells (1, 2, 3).
In addition to synaptobrevins, the brevin family includes other tissue-specific members such as cellubrevin (VAMP3), myobrevin (VAMP5), and endobrevin (VAMP8), which are expressed in various non-neuronal tissues (4, 5, 6). These isoforms exhibit distinct spatial expression profiles, suggesting specialized functions beyond the nervous system.
Two Synaptobrevin isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their regional distribution within the brain, indicating isoform-specific roles in neuroexocytosis (7).
Synaptobrevin1 (VAMP1) is supposed to be essential for the maintenance of nerve impulse transmission in neuromuscular synapses. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevin2 (VAMP2) is more abundant and widely distributed in the brain and has been shown to be mainly involved in the assembly of effective SNARE complexes, Ca2+-dependent SV exocytosis, and fast endocytosis in hippocampal synapses (8). It is also expressed in spinal cord dorsal horn neurons and implicated in inflammatory pain sensitization (9).
Synaptobrevins are target molecules for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule and thereby disrupt neurotransmitter release (10).

1 ) Jahn R et al., Annu. Rev. Biochem. (1999)
2 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
3 ) Rossi V et al., Biol Cell (2004)
4 ) Volchuk A et al., Biochem J (1994)
5 ) Zeng Q et al., Mol. Biol. Cell (1998)
6 ) Advani RJ et al., J. Biol. Chem. (1998)
7 ) Raptis A et al., J Chem Neuroanat (2005)
8 ) Yan C et al., Front Mol Neurosci (2022)
9 ) Duan XL et al., Neuroscience (2020)
10 ) Kumar R et al., Int J Mol Sci (2025)

Protocols

Other products for this target

Cat. No. 104-2P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	104 204, 104 211, 104 211BT, 104 211C3, 104 211C5, 104 008, 104 318, 104 211AT594, 104 211AbOR, 104 211AbRED
Remarks	This control peptide consists of the synthetic peptide (amino terminus) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	104-2p.pdf

Synaptobrevin2 (VAMP2) peptide - 104-2P