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Synaptobrevin 2 antibody - 104 211AT594

Synaptobrevin 2 also known as Vamp 2 is a major vesicle protein involved in fusion
Mouse monoclonal purified IgG
Cat. No.: 104 211AT594
Amount: 50 µg
Price: $465.00
Cat. No. 104 211AT594 50 µg purified IgG, lyophilized, fluorescence-labeled with ATTO® 594

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established CyDye fluorescence detection systems can be used.
ATTO® 550 has similar exitation and emission properties as Cy3, and ATTO® 647N as Cy5.
ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®594 is also recommended for PALM and dSTORM high resolution microscopy.

ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
CyDyes, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label ATTO 594
Clone 69.1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Epitop Epitop: AA 2 to 17 from rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity K.O.
Matching control protein/peptide 104-2P
Data sheet 104_211at594.pdf
Cat. No.: 104 211AT594
Amount: 50 µg
Price: $465.00
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin 1 (VAMP 1 or p18-1) and synaptobrevin 2 (VAMP 2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin 1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP 3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.

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