Synaptic Systems - Synaptobrevin2

Cat. No.: 104 008

Amount: 50 µg

Price: $420.00

Cat. No. 104 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes AP staining gallery IP: yes (see remarks) ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 5000 gallery
Clone	Rb69.1
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity	Reacts with: mouse (P63044), rat (P63045), human (P63027), hamster. No signal: chicken, zebrafish. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	104-2P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 69.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. Important: Although the epitope is still present in the Botox B cleavage product (aa 1 - 76), it is not recognized, probably due to conformational masking of the epitope. IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.
Data sheet	104_008.pdf

References for Synaptobrevin2 - 104 008

Disrupting stroke-induced GAT-1-syntaxin1A interaction promotes functional recovery after stroke.
Lin YH, Wu F, Li TY, Lin L, Gao F, Zhu LJ, Xu XM, Chen MY, Hou YL, Zhang CJ, Wu HY, et al.
Cell reports. Medicine (2024) 511: 101789. 104 008 WB; tested species: mouse

Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 104 008 WB; tested species: mouse

Cat. No.: 104 008

Amount: 50 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.

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2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Edelmann L et al., EMBO J. (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) McMahon HT et al., Nature (1993)
7 ) Archer BT et al., J. Biol. Chem. (1990)
8 ) Südhof TC et al., Neuron (1989)
9 ) Elferink LA et al., J. Biol. Chem. (1989)
10 ) Baumert M et al., EMBO J. (1989)

Protocols

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Synaptobrevin2 (VAMP2) antibody - 104 008 K.O.

References for Synaptobrevin2 - 104 008