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Synaptobrevin2 antibody - 104 008 K.O.

Synaptobrevin 2 also known as Vamp 2 is a major vesicle protein involved in fusion
Rabbit monoclonal recombinant IgG
Cat. No.: 104 008
Amount: 50 µg
Price: $415.00
Cat. No. 104 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes AP staining gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 5000 gallery  
Clone Rb69.1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: mouse (P63044), rat (P63045), human (P63027), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 104-2P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 69.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.

Data sheet 104_008.pdf

References for Synaptobrevin2 - 104 008

Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 104 008 WB; tested species: mouse
MINSTED nanoscopy enters the Ångström localization range.
Weber M, von der Emde H, Leutenegger M, Gunkel P, Sambandan S, Khan TA, Keller-Findeisen J, Cordes VC, Hell SW
Nature biotechnology (2023) 414: 569-576. 104 008 ICC; tested species: rat
Extracellular vesicle biomarkers for cognitive impairment in Parkinson's disease.
Blommer J, Pitcher T, Mustapic M, Eren E, Yao PJ, Vreones MP, Pucha KA, Dalrymple-Alford J, Shoorangiz R, Meissner WG, Anderson T, et al.
Brain : a journal of neurology (2023) 1461: 195-208. 104 008 ICC; tested species: human
NgCAM and VAMP2 Reveal that Direct Delivery and Dendritic Degradation Maintain Axonal Polarity.
Nabb AT, Bentley M
Molecular biology of the cell (2021) : mbcE21080425. 104 008 ICC; tested species: rat
Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology.
Heinrich L, Zafar F, Morato Torres CA, Singh J, Khan A, Chen MY, Hempel C, Nikulina N, Mulholland J, Braubach O, Schüle B, et al.
Journal of neuroscience methods (2022) : 109653. 104 008 Codex-PC; tested species: human
Cat. No.: 104 008
Amount: 50 µg
Price: $415.00
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 104 008 WB; tested species: mouse
MINSTED nanoscopy enters the Ångström localization range.
Weber M, von der Emde H, Leutenegger M, Gunkel P, Sambandan S, Khan TA, Keller-Findeisen J, Cordes VC, Hell SW
Nature biotechnology (2023) 414: 569-576. 104 008 ICC; tested species: rat
Extracellular vesicle biomarkers for cognitive impairment in Parkinson's disease.
Blommer J, Pitcher T, Mustapic M, Eren E, Yao PJ, Vreones MP, Pucha KA, Dalrymple-Alford J, Shoorangiz R, Meissner WG, Anderson T, et al.
Brain : a journal of neurology (2023) 1461: 195-208. 104 008 ICC; tested species: human
NgCAM and VAMP2 Reveal that Direct Delivery and Dendritic Degradation Maintain Axonal Polarity.
Nabb AT, Bentley M
Molecular biology of the cell (2021) : mbcE21080425. 104 008 ICC; tested species: rat
Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology.
Heinrich L, Zafar F, Morato Torres CA, Singh J, Khan A, Chen MY, Hempel C, Nikulina N, Mulholland J, Braubach O, Schüle B, et al.
Journal of neuroscience methods (2022) : 109653. 104 008 Codex-PC; tested species: human
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.