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Synaptobrevin 2 antibody - 104 211BT

Synaptobrevin2 also known as Vamp2 is a major vesicle protein involved in fusion
Mouse monoclonal purified IgG
Cat. No.: 104 211BT
Amount: 50 µg
Price: $465.00
Cat. No. 104 211BT 50 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 10000 up to 1 : 100000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 500 gallery  
Label biotin
Clone 69.1
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Epitop Epitop: AA 2 to 17 from rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity Specific for VAMP 2. No cross-reactivity to VAMP 1 and VAMP 3. K.O.
Matching control protein/peptide 104-2P
Remarks

Although the epitope for this antibody is still present in the Botulinumtoxin B cleavage product (aa 1 - 76), it is not recognized for unknown reasons.
IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.

Data sheet 104_211bt.pdf

References for Synaptobrevin 2 - 104 211BT

Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 104 211BT WB, UPTAKE; tested species: rat
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 104 211BT WB, UPTAKE; tested species: rat
Cat. No.: 104 211BT
Amount: 50 µg
Price: $465.00
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 104 211BT WB, UPTAKE; tested species: rat
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 104 211BT WB, UPTAKE; tested species: rat
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin 1 (VAMP 1 or p18-1) and synaptobrevin 2 (VAMP 2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin 1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP 3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.