Synaptic Systems - Synaptobrevin2

Cat. No.: 104 211AbOR

Amount: 50 µg

Price: $465.00

ICC

Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Edelmann L et al., EMBO J. (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) McMahon HT et al., Nature (1993)
7 ) Archer BT et al., J. Biol. Chem. (1990)
8 ) Südhof TC et al., Neuron (1989)
9 ) Elferink LA et al., J. Biol. Chem. (1989)
10 ) Baumert M et al., EMBO J. (1989)

Protocols

ICC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 104 211AbOR	50 µg purified IgG, lyophilized, fluorescence-labeled with abberior STAR ORANGE. abberior STAR^® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR^® dyes established fluorescence detection systems can be used. abberior STAR^® 635P: λ_ex 638 nm / λ_em 651 nm abberior STAR^® ORANGE: λ_ex 589 nm / λ_em 616 nm abberior STAR^® RED: λ_ex 638 nm / λ_em 655 nm This product or portions thereof is manufactured under license from abberior GmbH. abberior STAR is a trademark of abberior GmbH, Goettingen/Germany. Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet ELISA: N/A Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label	abberior STAR ORANGE
Clone	69.1
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity	Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster. No signal: chicken, zebrafish. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	104-2P
Remarks	ICC: This antibody conjugate is especially suitable for high resolution STED microscopy.
Data sheet	104_211abor.pdf

Synaptobrevin2 antibody - 104 211AbOR K.O.