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Synaptobrevin 2 antibody - 104 318

Synaptobrevin 2 also known as Vamp 2 is a major vesicle protein involved in fusion
Guinea pig monoclonal recombinant IgG
Cat. No.: 104 318
Amount: 50 µg
Price: $415.00
Cat. No. 104 318 50 µg purified recombinant IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 2000 gallery  
IHC-P: 1 : 400 up to 1 : 1000 gallery  
Clone Gp69.1
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Epitop Epitop: AA 2 to 17 from rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: mouse (P63044), rat (P63045), human (P63027), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
Specificity Specific for VAMP 2. No cross-reactivity to VAMP 1 and VAMP 3. K.O.
Matching control protein/peptide 104-2P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 69.1. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents.
The antibody has been expressed in mammalian cells.
IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.

Data sheet 104_318.pdf

References for Synaptobrevin 2 - 104 318

VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 104 318 ICC; tested species: rat
Cat. No.: 104 318
Amount: 50 µg
Price: $415.00
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 104 318 ICC; tested species: rat
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin 1 (VAMP 1 or p18-1) and synaptobrevin 2 (VAMP 2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin 1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP 3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.