Synaptic Systems - Synaptobrevin2

Cat. No.: 104 204

Amount: 100 µl

Price: $370.00

Cat. No. 104 204	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity	Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster. No signal: chicken. Other species not tested yet.
Matching control protein/peptide	104-2P
Data sheet	104_204.pdf

References for Synaptobrevin2 - 104 204

The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity.
Torrente D, Su EJ, Citalán-Madrid AF, Schielke GP, Magaoay D, Warnock M, Stevenson T, Mann K, Lesept F, Delétage N, Blanc M, et al.
Journal of neuroinflammation (2025) 221: 8. 104 204 IHC, WB; tested species: mouse

Rbfox1 Regulates Synaptic Transmission through the Inhibitory Neuron-Specific vSNARE Vamp1.
Vuong CK, Wei W, Lee JA, Lin CH, Damianov A, de la Torre-Ubieta L, Halabi R, Otis KO, Martin KC, O'Dell TJ, Black DL, et al.
Neuron (2018) 981: 127-141.e7. 104 204 WB, ICC; tested species: mouse

Septin 7 reduces nonmuscle myosin IIA activity in the SNAP23 complex and hinders GLUT4 storage vesicle docking and fusion.
Wasik AA, Dumont V, Tienari J, Nyman TA, Fogarty CL, Forsblom C, Lehto M, Lehtonen E, Groop PH, Lehtonen S
Experimental cell research (2017) 3502: 336-348. 104 204 WB, IP, ICC

Cat. No.: 104 204

Amount: 100 µl

Price: $370.00

ICC

IHC

IHC-P

Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin1 (VAMP1 or p18-1) and synaptobrevin2 (VAMP2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.

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2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Edelmann L et al., EMBO J. (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) McMahon HT et al., Nature (1993)
7 ) Archer BT et al., J. Biol. Chem. (1990)
8 ) Südhof TC et al., Neuron (1989)
9 ) Elferink LA et al., J. Biol. Chem. (1989)
10 ) Baumert M et al., EMBO J. (1989)

Protocols

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Synaptobrevin2 (VAMP2) antibody - 104 204

References for Synaptobrevin2 - 104 204