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Synaptobrevin 2 antibody - 104 204

Synaptobrevin2 also known as Vamp2 is a major vesicle protein involved in fusion
Guinea pig polyclonal antiserum
Cat. No.: 104 204
Amount: 100 µl
Price: $350.00
Cat. No. 104 204 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptobrevin2 (UniProt Id: P63045)
Reactivity Reacts with: human (P63027), rat (P63045), mouse (P63044), hamster.
No signal: chicken.
Other species not tested yet.
Specificity Specific for VAMP 2, no cross reactivity to VAMP 1 and VAMP 3.
Matching control protein/peptide 104-2P
Data sheet 104_204.pdf

References for Synaptobrevin 2 - 104 204

Rbfox1 Regulates Synaptic Transmission through the Inhibitory Neuron-Specific vSNARE Vamp1.
Vuong CK, Wei W, Lee JA, Lin CH, Damianov A, de la Torre-Ubieta L, Halabi R, Otis KO, Martin KC, O'Dell TJ, Black DL, et al.
Neuron (2018) 981: 127-141.e7. 104 204 WB, ICC; tested species: mouse
Septin 7 reduces nonmuscle myosin IIA activity in the SNAP23 complex and hinders GLUT4 storage vesicle docking and fusion.
Wasik AA, Dumont V, Tienari J, Nyman TA, Fogarty CL, Forsblom C, Lehto M, Lehtonen E, Groop PH, Lehtonen S
Experimental cell research (2017) 3502: 336-348. 104 204 WB, IP, ICC
Septin 7 reduces nonmuscle myosin IIA activity in the SNAP23 complex and hinders GLUT4 storage vesicle docking and fusion.
Wasik AA, Dumont V, Tienari J, Nyman TA, Fogarty CL, Forsblom C, Lehto M, Lehtonen E, Groop PH, Lehtonen S
Experimental cell research (2017) 3502: 336-348. 104 204 WB, IP, ICC
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 104 204 ICC; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 104 204 ICC; tested species: mouse
Rbfox1 Regulates Synaptic Transmission through the Inhibitory Neuron-Specific vSNARE Vamp1.
Vuong CK, Wei W, Lee JA, Lin CH, Damianov A, de la Torre-Ubieta L, Halabi R, Otis KO, Martin KC, O'Dell TJ, Black DL, et al.
Neuron (2018) 981: 127-141.e7. 104 204 WB, ICC; tested species: mouse
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 104 204 ICC
Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin.
van Stegen B, Dagar S, Gottmann K
Scientific reports (2017) 7: 40865. 104 204 ICC
Septin 7 reduces nonmuscle myosin IIA activity in the SNAP23 complex and hinders GLUT4 storage vesicle docking and fusion.
Wasik AA, Dumont V, Tienari J, Nyman TA, Fogarty CL, Forsblom C, Lehto M, Lehtonen E, Groop PH, Lehtonen S
Experimental cell research (2017) 3502: 336-348. 104 204 WB, IP, ICC
Evidence for glutamate as a neuroglial transmitter within sensory ganglia.
Kung LH, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara PT, Jasmin L
PloS one (2013) 87: e68312. 104 204 IHC
Cat. No.: 104 204
Amount: 100 µl
Price: $350.00
Rbfox1 Regulates Synaptic Transmission through the Inhibitory Neuron-Specific vSNARE Vamp1.
Vuong CK, Wei W, Lee JA, Lin CH, Damianov A, de la Torre-Ubieta L, Halabi R, Otis KO, Martin KC, O'Dell TJ, Black DL, et al.
Neuron (2018) 981: 127-141.e7. 104 204 WB, ICC; tested species: mouse
Septin 7 reduces nonmuscle myosin IIA activity in the SNAP23 complex and hinders GLUT4 storage vesicle docking and fusion.
Wasik AA, Dumont V, Tienari J, Nyman TA, Fogarty CL, Forsblom C, Lehto M, Lehtonen E, Groop PH, Lehtonen S
Experimental cell research (2017) 3502: 336-348. 104 204 WB, IP, ICC
Septin 7 reduces nonmuscle myosin IIA activity in the SNAP23 complex and hinders GLUT4 storage vesicle docking and fusion.
Wasik AA, Dumont V, Tienari J, Nyman TA, Fogarty CL, Forsblom C, Lehto M, Lehtonen E, Groop PH, Lehtonen S
Experimental cell research (2017) 3502: 336-348. 104 204 WB, IP, ICC
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 104 204 ICC; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 104 204 ICC; tested species: mouse
Rbfox1 Regulates Synaptic Transmission through the Inhibitory Neuron-Specific vSNARE Vamp1.
Vuong CK, Wei W, Lee JA, Lin CH, Damianov A, de la Torre-Ubieta L, Halabi R, Otis KO, Martin KC, O'Dell TJ, Black DL, et al.
Neuron (2018) 981: 127-141.e7. 104 204 WB, ICC; tested species: mouse
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 104 204 ICC
Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin.
van Stegen B, Dagar S, Gottmann K
Scientific reports (2017) 7: 40865. 104 204 ICC
Septin 7 reduces nonmuscle myosin IIA activity in the SNAP23 complex and hinders GLUT4 storage vesicle docking and fusion.
Wasik AA, Dumont V, Tienari J, Nyman TA, Fogarty CL, Forsblom C, Lehto M, Lehtonen E, Groop PH, Lehtonen S
Experimental cell research (2017) 3502: 336-348. 104 204 WB, IP, ICC
Evidence for glutamate as a neuroglial transmitter within sensory ganglia.
Kung LH, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara PT, Jasmin L
PloS one (2013) 87: e68312. 104 204 IHC
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin 1 (VAMP 1 or p18-1) and synaptobrevin 2 (VAMP 2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin 1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP 3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.