Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

GAD 2 / GAD 65 protein - 198-1P

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
control protein
Cat. No.: 198-1P
Amount: 100 µg
Price: $90.00
Cat. No. 198-1P 100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Immunogen Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 198 102, 198 103, 198 104, 198 111, 198 106, 198 108
Remarks

This control peptide consists of the recombinant protein (aa 3 - 96) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 198-1p.pdf
Cat. No.: 198-1P
Quantity: 100 µg
Price: $90.00
Background

The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.

Protocols