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GAD 2 / GAD 65 antibody - 198 103

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Polyclonal rabbit purified antibody
Cat. No.: 198 103
Amount: 50 µg
Price: $450.00
Cat. No. 198 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 2000 gallery  
IHC: 1 : 1000 gallery  
IHC-P/FFPE: 1 : 1000 gallery  
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)
Reactivity Reacts with: rat (P18088), mouse (P48318).
Other species not tested yet.
Specificity Specific for GAD 2 / GAD 65.
Matching control protein/peptide 198-1P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 198 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 198_103.pdf

References for GAD 2 / GAD 65 - 198 103

Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition.
Saliba RS, Gu Z, Yan Z, Moss SJ
The Journal of biological chemistry (2009) 28447: 32544-50. 198 103 WB
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 198 103 IHC
Cat. No.: 198 103
Quantity: 50 µg
Price: $450.00
Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition.
Saliba RS, Gu Z, Yan Z, Moss SJ
The Journal of biological chemistry (2009) 28447: 32544-50. 198 103 WB
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 198 103 IHC
Background

The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.