|Cat. No. 198 103||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
ICC: 1 : 2000 gallery
IHC: 1 : 1000 gallery
IHC-P/FFPE: 1 : 1000 gallery
ELISA: yes (see remarks)
|Immunogen||Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)|
Reacts with: rat (P18088), mouse (P48318).
Other species not tested yet.
|Specificity||Specific for GAD 2 / GAD 65.|
|Matching control protein/peptide||198-1P|
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 198 111 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.