Cat. No.: 198 104
Amount: 100 µl
Price:
$350.00
| Cat. No. 198 104 | 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 gallery IHC-P/FFPE: 1 : 300 gallery |
| Immunogen | Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320) |
| Reactivity |
Reacts with: human (Q99259), rat (P18088), mouse (P48318). Other species not tested yet. |
| Specificity | Specific for GAD 2 / GAD 65. |
| Matching control protein/peptide | 198-1P |
| Remarks |
WB: The Guinea pig antiserum is less sensitive than the rabbit antiserum (cat. no. 198 102). |
| Data sheet | 198_104.pdf |
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The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.