Synaptic Systems

Cat. No.: 198 104

Amount: 100 µl

Price: $375.00

Cat. No. 198 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 300 gallery ExM: 1 : 1000
Immunogen	Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)
Reactivity	Reacts with: human (Q99259), rat (P18088), mouse (P48318). Other species not tested yet.
Specificity	Specific for GAD 2 / GAD 65.
Matching control protein/peptide	198-1P
Remarks	WB: The Guinea pig antiserum is less sensitive than the rabbit antiserum (cat. no. 198 102).
Data sheet	198_104.pdf

References for GAD2 - 198 104

WB (1)
ICC (8)
IHC (11)
ExM (1)

Nrg1 haploinsufficiency alters inhibitory cortical circuits.
Navarro-Gonzalez C, Carceller H, Benito Vicente M, Serra I, Navarrete M, Domínguez-Canterla Y, Rodríguez-Prieto Á, González-Manteiga A, Fazzari P
Neurobiology of disease (2021) 157: 105442. 198 104 WB, IHC; tested species: mouse

Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 198 104 ICC; tested species: mouse

Regulation of dendrite morphology and excitatory synapse formation by zDHHC15.
Shah BS, Shimell JJ, Bamji SX
Journal of cell science (2019) 13213: . 198 104 ICC; tested species: rat

The X-Linked Intellectual Disability Gene Zdhhc9 Is Essential for Dendrite Outgrowth and Inhibitory Synapse Formation.
Shimell JJ, Shah BS, Cain SM, Thouta S, Kuhlmann N, Tatarnikov I, Jovellar DB, Brigidi GS, Kass J, Milnerwood AJ, Snutch TP, et al.
Cell reports (2019) 298: 2422-2437.e8. 198 104 ICC; tested species: rat

Diazepam Accelerates GABAAR Synaptic Exchange and Alters Intracellular Trafficking.
Lorenz-Guertin JM, Bambino MJ, Das S, Weintraub ST, Jacob TC
Frontiers in cellular neuroscience (2019) 13: 163. 198 104 ICC; tested species: rat

Depolarizing, inhibitory GABA type A receptor activity regulates GABAergic synapse plasticity via ERK and BDNF signaling.
Brady ML, Pilli J, Lorenz-Guertin JM, Das S, Moon CE, Graff N, Jacob TC
Neuropharmacology (2018) 128: 324-339. 198 104 ICC; tested species: rat

Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 198 104 ICC

Plexin-B3 suppresses excitatory and promotes inhibitory synapse formation in rat hippocampal neurons.
Laht P, Tammaru E, Otsus M, Rohtla J, Tiismus L, Veske A
Experimental cell research (2015) 3352: 269-78. 198 104 ICC

Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 198 104 ICC; tested species: mouse

Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord.
Nakamura Y, Kurabe M, Matsumoto M, Sato T, Miyashita S, Hoshina K, Kamiya Y, Tainaka K, Matsuzawa H, Ohno N, Ueno M, et al.
eLife (2023) 12: . 198 104 IHC, ExM; tested species: mouse

Control of contextual memory through interneuronal α5-GABAA receptors.
Zhu M, Abdulzahir A, Perkins MG, Chu CC, Krause BM, Casey C, Lennertz R, Ruhl D, Hentschke H, Nagarajan R, Chapman ER, et al.
PNAS nexus (2023) 24: pgad065. 198 104 IHC; tested species: mouse

Optogenetic "low-theta" pacing of the septohippocampal circuit is sufficient for spatial goal finding and is influenced by behavioral state and cognitive demand.
Mouchati PR, Kloc ML, Holmes GL, White SL, Barry JM
Hippocampus (2020) 3011: 1167-1193. 198 104 IHC; tested species: rat

Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex.
Modol L, Bollmann Y, Tressard T, Baude A, Che A, Duan ZRS, Babij R, De Marco García NV, Cossart R
Neuron (2020) 1051: 93-105.e4. 198 104 IHC; tested species: mouse

GABA bouton subpopulations in the human dentate gyrus are differentially altered in mesial temporal lobe epilepsy.
Alhourani A, Fish KN, Wozny TA, Sudhakar V, Hamilton RL, Richardson RM
Journal of neurophysiology (2020) 1231: 392-406. 198 104 IHC; tested species: human

Early Sociability and Social Memory Impairment in the A53T Mouse Model of Parkinson's Disease Are Ameliorated by Chemogenetic Modulation of Orexin Neuron Activity.
Stanojlovic M, Pallais Yllescas JP, Vijayakumar A, Kotz C
Molecular neurobiology (2019) : . 198 104 IHC; tested species: mouse

Lower glutamic acid decarboxylase 65-kDa isoform messenger RNA and protein levels in the prefrontal cortex in schizoaffective disorder but not schizophrenia.
Glausier JR, Kimoto S, Fish KN, Lewis DA
Biological psychiatry (2015) 772: 167-76. 198 104 IHC

Involvement of the cannabinoid CB1 receptor in modulation of dopamine output in the prefrontal cortex associated with food restriction in rats.
Dazzi L, Talani G, Biggio F, Utzeri C, Lallai V, Licheri V, Lutzu S, Mostallino MC, Secci PP, Biggio G, Sanna E, et al.
PloS one (2014) 93: e92224. 198 104 IHC

Expression pattern of Kv11 (Ether à-go-go-related gene; erg) K+ channels in the mouse retina.
Cordeiro S, Guseva D, Wulfsen I, Bauer CK
PloS one (2011) 612: e29490. 198 104 IHC; tested species: mouse

Characterization of Tissue Plasminogen Activator Expression and Trafficking in the Adult Murine Brain.
Stevenson TK, Lawrence DA
eNeuro () 54: . 198 104 IHC; tested species: mouse

Cat. No.: 198 104

Amount: 100 µl

Price: $375.00

References
WB (1)
ICC (8)
IHC (11)
ExM (1)

Regulation of dendrite morphology and excitatory synapse formation by zDHHC15.
Shah BS, Shimell JJ, Bamji SX
Journal of cell science (2019) 13213: . 198 104 ICC; tested species: rat

Characterization of Tissue Plasminogen Activator Expression and Trafficking in the Adult Murine Brain.
Stevenson TK, Lawrence DA
eNeuro () 54: . 198 104 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

The glutamic acid decarboxylases GAD1 and GAD2, also referred to as GAD67, and GAD65 respectively, synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. Therefore, GADs are widely used markers for the GABAergic system (1). The hydrophilic GAD1 can heterodimerize with the membrane achored GAD2 and a part of GAD1 is targeted to inhibitory nerve terminals by this mechanism (2). Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule (3).
GAD1 and 2 also occur in rodent pancreatic islets of Langerhans, whereas human islets mainly express GAD2 which has been identified as a major autoantigen in type 1 diabetes (3).

1 ) Tamamaki N et al., J. Comp. Neurol. (2003)
2 ) Kanaani J et al., J. Biol. Chem. (1999)
3 ) Kim J et al., Diabetes (1993)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

GAD2 (GAD65) antibody - 198 104

References for GAD2 - 198 104