|Cat. No. 198 104||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 500 up to 1 : 2000 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 300 gallery
|Immunogen||Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)|
Reacts with: human (Q99259), rat (P18088), mouse (P48318).
Other species not tested yet.
|Specificity||Specific for GAD 2 / GAD 65.|
|Matching control protein/peptide||198-1P|
WB: The Guinea pig antiserum is less sensitive than the rabbit antiserum (cat. no. 198 102).
The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.