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GAD 2 / GAD 65 antibody - 198 111

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Monoclonal mouse purified IgG
Cat. No.: 198 111
Amount: 100 µg
Price: $415.00
Cat. No. 198 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: not tested yet
ELISA: yes (see remarks)
Clone 26H1D1
Subtype IgG3 (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)
Epitop Epitop: AA 3 to 17 from mouse GAD2 (UniProt Id: P48320)
Reactivity Reacts with: rat (Q05683), mouse (P48320).
Other species not tested yet.
Specificity Specific for GAD 2 / GAD 65
Matching control protein/peptide 198-1P
Remarks

WB: This antibody is less sensitive than the rabbit antibody cat. no. 198 102.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 198 103 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 198_111.pdf

References for GAD 2 / GAD 65 - 198 111

ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 198 111 ICC; tested species: human
Cardiolipin exposure on the outer mitochondrial membrane modulates α-synuclein.
Ryan T, Bamm VV, Stykel MG, Coackley CL, Humphries KM, Jamieson-Williams R, Ambasudhan R, Mosser DD, Lipton SA, Harauz G, Ryan SD, et al.
Nature communications (2018) 91: 817. 198 111 ICC; tested species: human
ELKS controls the pool of readily releasable vesicles at excitatory synapses through its N-terminal coiled-coil domains.
Held RG, Liu C, Kaeser PS
eLife (2016) 5: . 198 111 ICC
Electrical Responses and Spontaneous Activity of Human iPS-Derived Neuronal Networks Characterized for 3-month Culture with 4096-Electrode Arrays.
Amin H, Maccione A, Marinaro F, Zordan S, Nieus T, Berdondini L
Frontiers in neuroscience (2016) 10: 121. 198 111 ICC; tested species: human
An E3-ligase-based method for ablating inhibitory synapses.
Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts RW, Trinh le A, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, et al.
Nature methods (2016) 138: 673-8. 198 111 ICC; tested species: mouse
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry (2015) 29037: 22593-601. 198 111 ICC
Evaluation of established human iPSC-derived neurons to model neurodegenerative diseases.
Meneghello G, Verheyen A, Van Ingen M, Kuijlaars J, Tuefferd M, Van Den Wyngaert I, Nuydens R
Neuroscience (2015) 301: 204-12. 198 111 ICC; tested species: human
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 198 111 ICC
Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons.
Coley AA, Ruffin VA, Moss FJ, Hopfer U, Boron WF
Neuroscience (2013) 246: 451-67. 198 111 ICC; tested species: mouse
Neural progenitors derived from human induced pluripotent stem cells survive and differentiate upon transplantation into a rat model of amyotrophic lateral sclerosis.
Popescu IR, Nicaise C, Liu S, Bisch G, Knippenberg S, Daubie V, Bohl D, Pochet R
Stem cells translational medicine (2013) 23: 167-74. 198 111 ICC; tested species: human
Cat. No.: 198 111
Quantity: 100 µg
Price: $415.00
ASCL1- and DLX2-induced GABAergic neurons from hiPSC-derived NPCs.
Barretto N, Zhang H, Powell SK, Fernando MB, Zhang S, Flaherty EK, Ho SM, Slesinger PA, Duan J, Brennand KJ
Journal of neuroscience methods (2020) 334: 108548. 198 111 ICC; tested species: human
Cardiolipin exposure on the outer mitochondrial membrane modulates α-synuclein.
Ryan T, Bamm VV, Stykel MG, Coackley CL, Humphries KM, Jamieson-Williams R, Ambasudhan R, Mosser DD, Lipton SA, Harauz G, Ryan SD, et al.
Nature communications (2018) 91: 817. 198 111 ICC; tested species: human
ELKS controls the pool of readily releasable vesicles at excitatory synapses through its N-terminal coiled-coil domains.
Held RG, Liu C, Kaeser PS
eLife (2016) 5: . 198 111 ICC
Electrical Responses and Spontaneous Activity of Human iPS-Derived Neuronal Networks Characterized for 3-month Culture with 4096-Electrode Arrays.
Amin H, Maccione A, Marinaro F, Zordan S, Nieus T, Berdondini L
Frontiers in neuroscience (2016) 10: 121. 198 111 ICC; tested species: human
An E3-ligase-based method for ablating inhibitory synapses.
Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts RW, Trinh le A, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, et al.
Nature methods (2016) 138: 673-8. 198 111 ICC; tested species: mouse
Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling.
Pan PY, Marrs J, Ryan TA
The Journal of biological chemistry (2015) 29037: 22593-601. 198 111 ICC
Evaluation of established human iPSC-derived neurons to model neurodegenerative diseases.
Meneghello G, Verheyen A, Van Ingen M, Kuijlaars J, Tuefferd M, Van Den Wyngaert I, Nuydens R
Neuroscience (2015) 301: 204-12. 198 111 ICC; tested species: human
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 198 111 ICC
Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons.
Coley AA, Ruffin VA, Moss FJ, Hopfer U, Boron WF
Neuroscience (2013) 246: 451-67. 198 111 ICC; tested species: mouse
Neural progenitors derived from human induced pluripotent stem cells survive and differentiate upon transplantation into a rat model of amyotrophic lateral sclerosis.
Popescu IR, Nicaise C, Liu S, Bisch G, Knippenberg S, Daubie V, Bohl D, Pochet R
Stem cells translational medicine (2013) 23: 167-74. 198 111 ICC; tested species: human
Background

The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.