|Cat. No. 198 111||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 500 gallery
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: not tested yet
ELISA: yes (see remarks)
|Subtype||IgG3 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)|
Epitop: AA 3 to 17 from mouse GAD2 (UniProt Id: P48320)
Reacts with: rat (Q05683), mouse (P48320).
Other species not tested yet.
|Specificity||Specific for GAD 2 / GAD 65|
|Matching control protein/peptide||198-1P|
WB: This antibody is less sensitive than the rabbit antibody cat. no. 198 102.
The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.