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GAD 2 / GAD 65 antibody - 198 102

GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
Polyclonal rabbit antiserum
Cat. No.: 198 102
Amount: 200 µl
Price: $350.00
Cat. No. 198 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: not recommended (see remarks)
IP: not tested yet
ICC: 1 : 2000 gallery  
IHC: not recommended
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)
Reactivity Reacts with: rat (P18088), mouse (P48318).
Other species not tested yet.
Specificity Specific for GAD 2 / GAD 65.
Matching control protein/peptide 198-1P
Remarks

WB: The affinity purified antibody is recommended.

Data sheet 198_102.pdf

References for GAD 2 / GAD 65 - 198 102

Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition.
Saliba RS, Gu Z, Yan Z, Moss SJ
The Journal of biological chemistry (2009) 28447: 32544-50. 198 102 WB
Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell.
Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, et al.
Nature metabolism (2019) 111: 1110-1126. 198 102 ICC, IHC; tested species: human,rat
Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.
Kaczor P, Rakus D, Mozrzymas JW
Frontiers in cellular neuroscience (2015) 9: 120. 198 102 ICC
Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell.
Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, et al.
Nature metabolism (2019) 111: 1110-1126. 198 102 ICC, IHC; tested species: human,rat
Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.
Wu W, Rahman MN, Guo J, Roy N, Xue L, Cahill CM, Zhang S, Jia Z
Journal of molecular cell biology (2015) 72: 168-79. 198 102 IHC
Cat. No.: 198 102
Quantity: 200 µl
Price: $350.00
Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition.
Saliba RS, Gu Z, Yan Z, Moss SJ
The Journal of biological chemistry (2009) 28447: 32544-50. 198 102 WB
Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell.
Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, et al.
Nature metabolism (2019) 111: 1110-1126. 198 102 ICC, IHC; tested species: human,rat
Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.
Kaczor P, Rakus D, Mozrzymas JW
Frontiers in cellular neuroscience (2015) 9: 120. 198 102 ICC
Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell.
Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, et al.
Nature metabolism (2019) 111: 1110-1126. 198 102 ICC, IHC; tested species: human,rat
Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.
Wu W, Rahman MN, Guo J, Roy N, Xue L, Cahill CM, Zhang S, Jia Z
Journal of molecular cell biology (2015) 72: 168-79. 198 102 IHC
Background

The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.

Protocols