Synaptic Systems

Cat. No.: 198 102

Amount: 200 µl

Price: $360.00

Cat. No. 198 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended (see remarks) IP: not tested yet ICC: 1 : 2000 gallery IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 3 to 96 from mouse GAD2 (UniProt Id: P48320)
Reactivity	Reacts with: rat (P18088), mouse (P48318). Other species not tested yet.
Specificity	Specific for GAD 2 / GAD 65.
Matching control protein/peptide	198-1P
Remarks	WB: The affinity purified antibody is recommended.
Data sheet	198_102.pdf

References for GAD2 - 198 102

Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition.
Saliba RS, Gu Z, Yan Z, Moss SJ
The Journal of biological chemistry (2009) 28447: 32544-50. 198 102 WB

Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell.
Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, et al.
Nature metabolism (2019) 111: 1110-1126. 198 102 ICC, IHC; tested species: human,rat

Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.
Kaczor P, Rakus D, Mozrzymas JW
Frontiers in cellular neuroscience (2015) 9: 120. 198 102 ICC

Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.
Wu W, Rahman MN, Guo J, Roy N, Xue L, Cahill CM, Zhang S, Jia Z
Journal of molecular cell biology (2015) 72: 168-79. 198 102 IHC

Cat. No.: 198 102

Amount: 200 µl

Price: $360.00

ICC

Background

The glutamic acid decarboxylases GAD 2, also referred to as GAD 65, and GAD 1 / GAD 67 synthesize γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. The hydrophilic GAD 1 can heterodimerize with the membrane achored GAD 2 and part of GAD 1 is targeted to inhibitory nerve terminals by this mechanisms. Although both proteins exhibit significant differences in their N-terminus they share high homology in the rest of the molecule.
GADs are a widely used markers for the GABAergic system. In type 1 diabetes GAD 1 has been identified as a major autoantigen.

1 ) Kanaani J et al., J. Cell. Sci. (2008)
2 ) Kanaani J et al., J. Biol. Chem. (1999)
3 ) Kim J et al., Diabetes (1993)
4 ) Tillakaratne NJ et al., J. Neurochem. (1992)

Protocols

ICC

OTHER

Preabsorbtion

Other products for this target

GAD2 (GAD65) antibody - 198 102

References for GAD2 - 198 102