|Cat. No. 160-0P||100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.|
|Immunogen||Recombinant protein corresponding to AA 1 to 196 from human Homer1 (UniProt Id: Q86YM7)|
|Recommended dilution||Optimal concentrations should be determined by the end-user.|
|Matching antibodies||160 002, 160 003, 160 004, 160 006, 160 011, 160 011BT, 160 008|
This control protein consists of the recombinant protein (aa 1 - 186 of human homer) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.