Homer1 antibody - 160 005

Homer1 , 2 and 3 are post synaptic density (PSD) proteins involved in the targeting of glutamate receptors

Guinea pig polyclonal purified antibody

Cat. No.: 160 005

Amount: 100 µl

Price: $460.00

Cat. No. 160 005	100 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the c-terminal half of human Homer 1b (UniProt Id: Q86YM7)
Reactivity	Reacts with: mouse (Q9Z2Y3), rat (Q9Z214), human (Q86YM7). Other species not tested yet.
Specificity	Specific for Homer 1. According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.
Matching control protein/peptide	160-0P
Data sheet	160_005.pdf

Cat. No.: 160 005

Amount: 100 µl

Price: $460.00

ICC

Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.

1 ) Kammermeier PJ et al., BMC Neurosci (2006)
2 ) Yamamoto K et al., Eur. J. Neurosci. (2005)
3 ) Thomas U et al., J. Neurochem. (2002)
4 ) Ango F et al., Mol. Cell. Neurosci. (2002)
5 ) Sala C et al., Neuron (2001)
6 ) Ciruela F et al., Mol. Cell. Neurosci. (2000)
7 ) Xiao B et al., Curr. Opin. Neurobiol. (2000)
8 ) Tu JC et al., Neuron (1999)
9 ) Brakeman PR et al., Nature (1997)
10 ) Shiraishi Y et al., J. Comp. Neurol. (2004)

Protocols

ICC

OTHER

Preabsorbtion

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