Synaptic Systems

Cat. No.: 160 006

Amount: 50 µg

Price: $410.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 160 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery ExM: 1 : 250 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to the N-terminal half of human Homer 1 (UniProt Id: Q86YM7)
Reactivity	Reacts with: human (Q86YM7), rat (Q9Z214), mouse (Q9Z2Y3). Other species not tested yet.
Specificity	Specific for Homer 1. According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.
Matching control protein/peptide	160-0P
Remarks	ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet	160_006.pdf

References for Homer1 - 160 006

Deep proteomics identifies shared molecular pathway alterations in synapses of patients with schizophrenia and bipolar disorder and mouse model.
Aryal S, Bonanno K, Song B, Mani DR, Keshishian H, Carr SA, Sheng M, Dejanovic B
Cell reports (2023) 425: 112497. 160 006 WB; tested species: human

Neuronopathic GBA1L444P Mutation Accelerates Glucosylsphingosine Levels and Formation of Hippocampal Alpha-Synuclein Inclusions.
Mahoney-Crane CL, Viswanathan M, Russell D, Curtiss RAC, Freire J, Bobba SS, Coyle SD, Kandebo M, Yao L, Wan BL, Hatcher NG, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 433: 501-521. 160 006 WB; tested species: mouse

Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.
Kareemo DJ, Winborn CS, Olah SS, Miller CN, Kim J, Kadgien CA, Actor-Engel HS, Ramsay HJ, Ramsey AM, Aoto J, Kennedy MJ, et al.
Nature communications (2024) 151: 10374. 160 006 ICC; tested species: rat

Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.
Lloyd BA, Han Y, Roth R, Zhang B, Aoto J
Nature communications (2023) 141: 4706. 160 006 ICC; tested species: mouse

Microglia-synapse engulfment via PtdSer-TREM2 ameliorates neuronal hyperactivity in Alzheimer's disease models.
Rueda-Carrasco J, Sokolova D, Lee SE, Childs T, Jurčáková N, Crowley G, De Schepper S, Ge JZ, Lachica JI, Toomey CE, Freeman OJ, et al.
The EMBO journal (2023) 4219: e113246. 160 006 ICC; tested species: rat

High-Content Screening of Synaptic Density Modulators in Primary Neuronal Cultures.
Coulon A, Siedlecki-Wullich D, Najdek C, Gelle C, Ayral AM, Demiautte F, Lambert E, Vandeputte A, Brodin P, Mendes T, Lambert JC, et al.
Current protocols (2023) 310: e904. 160 006 ICC; tested species: rat

Cationic peptides erase memories by removing synaptic AMPA receptors through endophilin-mediated endocytosis.
Beier K, Stokes E, Zhuang Y, Toledano M, Vasquez J, Azouz G, Hui M, Tyler I, Shi X, Aoto J
Research square (2023) : . 160 006 ICC, ExM; tested species: mouse

A genetic variant of the Wnt receptor LRP6 accelerates synapse degeneration during aging and in Alzheimer's disease.
Jones ME, Büchler J, Dufor T, Palomer E, Teo S, Martin-Flores N, Boroviak K, Metzakopian E, Gibb A, Salinas PC
Science advances (2023) 92: eabo7421. 160 006 ICC, IHC; tested species: mouse

Interleukin-4 receptor signaling modulates neuronal network activity.
Hanuscheck N, Thalman C, Domingues M, Schmaul S, Muthuraman M, Hetsch F, Ecker M, Endle H, Oshaghi M, Martino G, Kuhlmann T, et al.
The Journal of experimental medicine (2022) 2196: . 160 006 ICC, IHC; tested species: human,mouse

Inhibition of LRRK2 kinase activity promotes anterograde axonal transport and presynaptic targeting of α-synuclein.
Brzozowski CF, Hijaz BA, Singh V, Gcwensa NZ, Kelly K, Boyden ES, West AB, Sarkar D, Volpicelli-Daley LA
Acta neuropathologica communications (2021) 91: 180. 160 006 ICC, IHC, ExM; tested species: mouse

Pyk2 overexpression in postsynaptic neurons blocks amyloid β1-42-induced synaptotoxicity in microfluidic co-cultures.
Kilinc D, Vreulx AC, Mendes T, Flaig A, Marques-Coelho D, Verschoore M, Demiautte F, Amouyel P, , Eysert F, Dourlen P, et al.
Brain communications (2020) 22: fcaa139. 160 006 ICC; tested species: rat

Excitatory synaptic structural abnormalities produced by templated aggregation of α-syn in the basolateral amygdala.
Gcwensa NZ, Russell DL, Long KY, Brzozowski CF, Liu X, Gamble KL, Cowell RM, Volpicelli-Daley LA
Neurobiology of disease (2024) 199: 106595. 160 006 IHC; tested species: mouse

Age-related dysregulation of homeostatic control in neuronal microcircuits.
Radulescu CI, Doostdar N, Zabouri N, Melgosa-Ecenarro L, Wang X, Sadeh S, Pavlidi P, Airey J, Kopanitsa M, Clopath C, Barnes SJ, et al.
Nature neuroscience (2023) 2612: 2158-2170. 160 006 IHC; tested species: mouse

Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain.
Singh V, Menard MA, Serrano GE, Beach TG, Zhao HT, Riley-DiPaolo A, Subrahmanian N, LaVoie MJ, Volpicelli-Daley LA
Acta neuropathologica communications (2023) 111: 201. 160 006 IHC; tested species: human,mouse

Development and cadherin-mediated control of prefrontal corticostriatal projections in mice.
Mesías RE, Zaki Y, Guevara CA, Friedman LG, Hussein A, Therrien K, Magee AR, Tzavaras N, Del Valle P, Baxter MG, Huntley GW, et al.
iScience (2023) 2610: 108002. 160 006 IHC; tested species: mouse

Low-dose PLX5622 treatment prevents neuroinflammatory and neurocognitive sequelae after sepsis.
Mein N, von Stackelberg N, Wickel J, Geis C, Chung HY
Journal of neuroinflammation (2023) 201: 289. 160 006 IHC; tested species: mouse

Influenza vaccine is able to prevent neuroinflammation triggered by H7N7 IAV infection.
Demuth L, Ohm M, Michaelsen-Preusse K, Schulze K, Riese P, Guzmán CA, Korte M, Hosseini S
Frontiers in pharmacology (2023) 14: 1142639. 160 006 IHC; tested species: mouse

URMC-099 prophylaxis prevents hippocampal vascular vulnerability and synaptic damage in an orthopedic model of delirium superimposed on dementia.
Miller-Rhodes P, Li H, Velagapudi R, Chiang W, Terrando N, Gelbard HA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022) 366: e22343. 160 006 IHC; tested species: mouse

Pregabalin improves axon regeneration and motor outcome in a rodent stroke model.
Kugler C, Blank N, Matuskova H, Thielscher C, Reichenbach N, Lin TC, Bradke F, Petzold GC
Brain communications (2022) 44: fcac170. 160 006 IHC; tested species: mouse

Circulating Pro-Inflammatory Exosomes Worsen Stroke Outcomes in Aging.
Zhang H, Lin S, McElroy CL, Wang B, Jin D, Uteshev VV, Jin K
Circulation research (2021) 1297: e121-e140. 160 006 IHC; tested species: rat

Gabapentinoid treatment promotes corticospinal plasticity and regeneration following murine spinal cord injury.
Sun W, Larson MJ, Kiyoshi CM, Annett AJ, Stalker WA, Peng J, Tedeschi A
The Journal of clinical investigation (2020) 1301: 345-358. 160 006 IHC; tested species: mouse

Elevated protein synthesis in microglia causes autism-like synaptic and behavioral aberrations.
Xu ZX, Kim GH, Tan JW, Riso AE, Sun Y, Xu EY, Liao GY, Xu H, Lee SH, Do NY, Lee CH, et al.
Nature communications (2020) 111: 1797. 160 006 IHC; tested species: mouse

Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 160 006 IHC; tested species: mouse

Short-chain fatty acids improve post-stroke recovery via immunological mechanisms.
Sadler R, Cramer JV, Heindl S, Kostidis S, Betz D, Zuurbier KR, Northoff BH, Heijink M, Goldberg MP, Plautz EJ, Roth S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 160 006 IHC; tested species: mouse

Phagocyte-mediated synapse removal in cortical neuroinflammation is promoted by local calcium accumulation.
Jafari M, Schumacher AM, Snaidero N, Ullrich Gavilanes EM, Neziraj T, Kocsis-Jutka V, Engels D, Jürgens T, Wagner I, Weidinger JDF, Schmidt SS, et al.
Nature neuroscience (2021) 243: 355-367. 160 006 IHC-P; tested species: mouse

P2Y1 receptor blockade normalizes network dysfunction and cognition in an Alzheimer's disease model.
Reichenbach N, Delekate A, Breithausen B, Keppler K, Poll S, Schulte T, Peter J, Plescher M, Hansen JN, Blank N, Keller A, et al.
The Journal of experimental medicine (2018) : . 160 006 IHC-P; tested species: mouse

Protecting RNA quality for spatial transcriptomics while improving immunofluorescent staining quality.
Hahn N, Bens M, Kempfer M, Reißig C, Schmidl L, Geis C
Frontiers in neuroscience (2023) 17: 1198154. 160 006 IHC-Fr; tested species: mouse

Cat. No.: 160 006

Amount: 50 µg

Price: $410.00

ICC

IHC

IHC-P

ExM

Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.

1 ) Kammermeier PJ et al., BMC Neurosci (2006)
2 ) Yamamoto K et al., Eur. J. Neurosci. (2005)
3 ) Thomas U et al., J. Neurochem. (2002)
4 ) Ango F et al., Mol. Cell. Neurosci. (2002)
5 ) Sala C et al., Neuron (2001)
6 ) Ciruela F et al., Mol. Cell. Neurosci. (2000)
7 ) Xiao B et al., Curr. Opin. Neurobiol. (2000)
8 ) Tu JC et al., Neuron (1999)
9 ) Brakeman PR et al., Nature (1997)
10 ) Shiraishi Y et al., J. Comp. Neurol. (2004)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Homer1 antibody - 160 006

References for Homer1 - 160 006