Synaptic Systems

Cat. No.: 160 003

Amount: 50 µg

Price: $480.00

Cat. No. 160 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC: 1 : 200 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery ExM: yes DNA-PAINT: 1 : 500 iDISCO: yes ELISA: yes (see remarks)
Immunogen	Recombinant protein corresponding to the N-terminal half of human Homer 1 (UniProt Id: Q86YM7)
Reactivity	Reacts with: human (Q86YM7), rat (Q9Z214), mouse (Q9Z2Y3). Other species not tested yet.
Specificity	Specific for Homer 1. Cross-reactivity of the serum to Homer 2 and 3 was removed by pre-adsorption with Homer 2 (aa 1 - 176) and Homer 3 (aa 1 - 177).
Matching control protein/peptide	160-0P
Remarks	ICC: PFA fixation is recommended. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 160 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	160_003.pdf

References for Homer1 - 160 003

Expression of synaptic proteins and development of dendritic spines in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa.
Sobierajski E, Czubay K, Schmidt MR, Wiedenski S, Rettschlag S, Beemelmans C, Beemelmans C, Wahle P
Brain structure & function (2025) 2302: 38. 160 003 WB; tested species: pig

Mouse-derived Synaptosomes Trypsin Cleavage Assay to Characterize Synaptic Protein Sub-localization.
Shergill JK, Tehran DA
Bio-protocol (2025) 152: e5164. 160 003 WB; tested species: mouse

Synaptic signatures and disease vulnerabilities of layer 5 pyramidal neurons.
Marcassa G, Dascenco D, Lorente-Echeverría B, Daaboul D, Vandensteen J, Leysen E, Baltussen L, Howden AJM, de Wit J
Nature communications (2025) 161: 228. 160 003 WB, IHC; tested species: mouse

Mitigation of synaptic and memory impairments via F-actin stabilization in Alzheimer's disease.
P A H, Basavaraju N, Chandran M, Jaleel A, Bennett DA, Kommaddi RP
Alzheimer's research & therapy (2024) 161: 200. 160 003 WB, ICC; tested species: mouse

Neuron type-specific proteomics reveals distinct Shank3 proteoforms in iSPNs and dSPNs lead to striatal synaptopathy in Shank3B-/- mice.
Wang YZ, Perez-Rosello T, Smukowski SN, Surmeier DJ, Savas JN
Molecular psychiatry (2024) 298: 2372-2388. 160 003 WB; tested species: mouse

Development of MAPT S305 mutation human iPSC lines exhibiting elevated 4R tau expression and functional alterations in neurons and astrocytes.
Bowles KR, Pedicone C, Pugh DA, Oja LM, Sousa FH, Keavey LK, Fulton-Howard B, Weitzman SA, Liu Y, Chen JL, Disney MD, et al.
Cell reports (2024) 4312: 115013. 160 003 WB; tested species: human

Manipulation of DHPS activity affects dendritic morphology and expression of synaptic proteins in primary rat cortical neurons.
Cavalli P, Raffauf A, Passarella S, Helmuth M, Dieterich DC, Landgraf P
Frontiers in cellular neuroscience (2024) 18: 1465011. 160 003 WB, ICC; tested species: rat

Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 160 003 WB; tested species: human

Person-specific differences in ubiquitin-proteasome mediated proteostasis in human neurons.
Hsieh YC, Augur ZM, Arbery M, Ashour N, Barrett K, Pearse RV, Tio ES, Duong DM, Felsky D, De Jager PL, Bennett DA, et al.
Alzheimer's & dementia : the journal of the Alzheimer's Association (2024) : . 160 003 WB; tested species: human

High-content synaptic phenotyping in human cellular models reveals a role for BET proteins in synapse assembly.
Berryer MH, Rizki G, Nathanson A, Klein JA, Trendafilova D, Susco SG, Lam D, Messana A, Holton KM, Karhohs KW, Cimini BA, et al.
eLife (2023) 12: . 160 003 WB; tested species: human

Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice.
Monsorno K, Ginggen K, Ivanov A, Buckinx A, Lalive AL, Tchenio A, Benson S, Vendrell M, D'Alessandro A, Beule D, Pellerin L, et al.
Nature communications (2023) 141: 5749. 160 003 WB, IHC; tested species: mouse

Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.
Lloyd BA, Han Y, Roth R, Zhang B, Aoto J
Nature communications (2023) 141: 4706. 160 003 WB; tested species: mouse

Developmental Pb exposure increases AD risk via altered intracellular Ca2+ homeostasis in hiPSC-derived cortical neurons.
Xie J, Wu S, Szadowski H, Min S, Yang Y, Bowman AB, Rochet JC, Freeman JL, Yuan C
The Journal of biological chemistry (2023) : 105023. 160 003 WB, ICC; tested species: human

Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy.
Gozzelino L, Kochlamazashvili G, Baldassari S, Mackintosh AI, Licchetta L, Iovino E, Liu YC, Bennett CA, Bennett MF, Damiano JA, Zsurka G, et al.
Brain : a journal of neurology (2022) 1457: 2313-2331. 160 003 WB; tested species: mouse

Elevated ganglioside GM2 activator (GM2A) in human brain tissue reduces neurite integrity and spontaneous neuronal activity.
Hsieh YC, Negri J, He A, Pearse RV, Liu L, Duong DM, Chibnik LB, Bennett DA, Seyfried NT, Young-Pearse TL
Molecular neurodegeneration (2022) 171: 61. 160 003 WB, ICC; tested species: human

Nrg1 haploinsufficiency alters inhibitory cortical circuits.
Navarro-Gonzalez C, Carceller H, Benito Vicente M, Serra I, Navarrete M, Domínguez-Canterla Y, Rodríguez-Prieto Á, González-Manteiga A, Fazzari P
Neurobiology of disease (2021) 157: 105442. 160 003 WB; tested species: mouse

Missense mutation of Fmr1 results in impaired AMPAR-mediated plasticity and socio-cognitive deficits in mice.
Prieto M, Folci A, Poupon G, Schiavi S, Buzzelli V, Pronot M, François U, Pousinha P, Lattuada N, Abelanet S, Castagnola S, et al.
Nature communications (2021) 121: 1557. 160 003 WB, ICC; tested species: mouse

Overexpression of schizophrenia susceptibility factor human complement C4A promotes excessive synaptic loss and behavioral changes in mice.
Yilmaz M, Yalcin E, Presumey J, Aw E, Ma M, Whelan CW, Stevens B, McCarroll SA, Carroll MC
Nature neuroscience (2021) 242: 214-224. 160 003 WB, IHC; tested species: mouse

Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 160 003 WB; tested species: mouse

Proteome dynamics during homeostatic scaling in cultured neurons.
Dörrbaum AR, Alvarez-Castelao B, Nassim-Assir B, Langer JD, Schuman EM
eLife (2020) 9: . 160 003 WB; tested species: rat

Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum.
Zhang RS, Liakath-Ali K, Südhof TC
eLife (2020) 9: . 160 003 WB, IHC; tested species: mouse

SETD5 Regulates Chromatin Methylation State and Preserves Global Transcriptional Fidelity during Brain Development and Neuronal Wiring.
Sessa A, Fagnocchi L, Mastrototaro G, Massimino L, Zaghi M, Indrigo M, Cattaneo S, Martini D, Gabellini C, Pucci C, Fasciani A, et al.
Neuron (2019) : . 160 003 WB; tested species: mouse

Fluoxetine Suppresses Glutamate- and GABA-Mediated Neurotransmission by Altering SNARE Complex.
Lazarevic V, Mantas I, Flais I, Svenningsson P
International journal of molecular sciences (2019) 2017: . 160 003 WB; tested species: rat

LRRK2 modifies α-syn pathology and spread in mouse models and human neurons.
Bieri G, Brahic M, Bousset L, Couthouis J, Kramer NJ, Ma R, Nakayama L, Monbureau M, Defensor E, Schüle B, Shamloo M, et al.
Acta neuropathologica (2019) : . 160 003 WB; tested species: mouse

Neuronal KIF5b deletion induces striatum-dependent locomotor impairments and defects in membrane presentation of dopamine D2 receptors.
Cromberg LE, Saez TMM, Otero MG, Tomasella E, Alloatti M, Damianich A, Devoto VP, Ferrario J, Gelman D, Rubinstein M, Falzone TL, et al.
Journal of neurochemistry (2019) : . 160 003 WB; tested species: human

Hippocampal Memory Recovery After Acute Stress: A Behavioral, Morphological and Molecular Study.
Aguayo FI, Tejos-Bravo M, Díaz-Véliz G, Pacheco A, García-Rojo G, Corrales W, Olave FA, Aliaga E, Ulloa JL, Avalos AM, Román-Albasini L, et al.
Frontiers in molecular neuroscience (2018) 11: 283. 160 003 WB; tested species: rat

Homer1a drives homeostatic scaling-down of excitatory synapses during sleep.
Diering GH, Nirujogi RS, Roth RH, Worley PF, Pandey A, Huganir RL
Science (New York, N.Y.) (2017) 3556324: 511-515. 160 003 WB, IP; tested species: mouse

Distinct Defects in Synaptic Differentiation of Neocortical Neurons in Response to Prenatal Valproate Exposure.
Iijima Y, Behr K, Iijima T, Biemans B, Bischofberger J, Scheiffele P
Scientific reports (2016) 6: 27400. 160 003 WB

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 160 003 WB

Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 003 WB, EM; tested species: rat

SAM68 regulates neuronal activity-dependent alternative splicing of neurexin-1.
Iijima T, Wu K, Witte H, Hanno-Iijima Y, Glatter T, Richard S, Scheiffele P
Cell (2011) 1477: 1601-14. 160 003 WB; tested species: mouse

Cat. No.: 160 003

Amount: 50 µg

Price: $480.00

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Homer1 antibody - 160 003

References for Homer1 - 160 003