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Homer 1 - 160 011

A post synaptic density (PSD) protein involved in the targeting of glutamate receptors
Monoclonal mouse purified IgG
Cat. No.: 160 011
Amount: 100 µg
Price: $425.00
Cat. No. 160 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
EM: yes
ELISA: yes (see remarks)
Clone 2G8
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 196 from human Homer1 (UniProt Id: Q86YM7)
Epitop Epitop: AA 1 to 168 from human Homer1 (UniProt Id: Q86YM7)
Reactivity Reacts with: human (Q86YM7), rat (Q9Z214), mouse (Q9Z2Y3).
Other species not tested yet.
Specificity Specific for Homer 1. According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.
Matching control protein/peptide 160-0P
Remarks

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 160 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 160_011.pdf

References for Homer 1 - 160 011

Rho Guanine Nucleotide Exchange Factor 4 (Arhgef4) Deficiency Enhances Spatial and Object Recognition Memory.
Yoo KS, Lee K, Lee YS, Oh WJ, Kim HK
Experimental neurobiology (2020) 295: 334-343. 160 011 WB; tested species: mouse
Glutamate treatment mimics LTP- and LTD-like biochemical activity in viable synaptosome preparation.
Gharami K, Biswas SC
Neurochemistry international (2019) 134: 104655. 160 011 WB; tested species: mouse
JIP2 haploinsufficiency contributes to neurodevelopmental abnormalities in human pluripotent stem cell-derived neural progenitors and cortical neurons.
Roessler R, Goldmann J, Shivalila C, Jaenisch R
Life science alliance (2018) 14: e201800094. 160 011 WB; tested species: human
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 011 WB, EM
Neuroinflammation induces synaptic scaling through IL-1β-mediated activation of the transcriptional repressor REST/NRSF.
Buffolo F, Petrosino V, Albini M, Moschetta M, Carlini F, Floss T, Kerlero de Rosbo N, Cesca F, Rocchi A, Uccelli A, Benfenati F, et al.
Cell death & disease (2021) 122: 180. 160 011 ICC; tested species: mouse
A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids.
Renner H, Grabos M, Becker KJ, Kagermeier TE, Wu J, Otto M, Peischard S, Zeuschner D, TsyTsyura Y, Disse P, Klingauf J, et al.
eLife (2020) 9: . 160 011 ICC; tested species: human
Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F
Nanoscale (2020) 1218: 10226-10239. 160 011 ICC; tested species: rat
Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation.
Zhang K, Yu F, Zhu J, Han S, Chen J, Wu X, Chen Y, Shen T, Liao J, Guo W, Yang X, et al.
Cell reports (2020) 313: 107521. 160 011 ICC; tested species: human
Acute knockdown of Depdc5 leads to synaptic defects in mTOR-related epileptogenesis.
De Fusco A, Cerullo MS, Marte A, Michetti C, Romei A, Castroflorio E, Baulac S, Benfenati F
Neurobiology of disease (2020) : 104822. 160 011 ICC; tested species: mouse
Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses.
Mitlöhner J, Kaushik R, Niekisch H, Blondiaux A, Gee CE, Happel MFK, Gundelfinger E, Dityatev A, Frischknecht R, Seidenbecher C
Cells (2020) 92: . 160 011 ICC; tested species: rat
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 160 011 ICC; tested species: mouse
The RAB3-RIM Pathway Is Essential for the Release of Neuromodulators.
Persoon CM, Hoogstraaten RI, Nassal JP, van Weering JRT, Kaeser PS, Toonen RF, Verhage M
Neuron (2019) 1046: 1065-1080.e12. 160 011 ICC; tested species: mouse
Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course.
Esposito A, Falace A, Wagner M, Gal M, Mei D, Conti V, Pisano T, Aprile D, Cerullo MS, De Fusco A, Giovedì S, et al.
Brain : a journal of neurology (2019) : . 160 011 ICC; tested species: mouse
De novo mutations of the ATP6V1A gene cause developmental encephalopathy with epilepsy.
Fassio A, Esposito A, Kato M, Saitsu H, Mei D, Marini C, Conti V, Nakashima M, Okamoto N, Olmez Turker A, Albuz B, et al.
Brain : a journal of neurology (2018) 1416: 1703-1718. 160 011 ICC; tested species: rat
Constitutive Inactivation of the PRRT2 Gene Alters Short-Term Synaptic Plasticity and Promotes Network Hyperexcitability in Hippocampal Neurons.
Valente P, Romei A, Fadda M, Sterlini B, Lonardoni D, Forte N, Fruscione F, Castroflorio E, Michetti C, Giansante G, Valtorta F, et al.
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 160 011 ICC; tested species: mouse
Adenomatous polyposis coli-stimulated GEF 1 (Asef1) is a negative regulator of excitatory synaptic function.
Oh JY, Lim CS, Yoo KS, Park HJ, Park YS, Kim EG, Lee YS, Kaang BK, Kim HK
Journal of neurochemistry (2018) : . 160 011 ICC; tested species: rat
Systematic Three-Dimensional Coculture Rapidly Recapitulates Interactions between Human Neurons and Astrocytes.
Krencik R, Seo K, van Asperen JV, Basu N, Cvetkovic C, Barlas S, Chen R, Ludwig C, Wang C, Ward ME, Gan L, et al.
Stem cell reports (2017) 96: 1745-1753. 160 011 ICC
Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 160 011 ICC; tested species: mouse
Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.
Sloan SA, Darmanis S, Huber N, Khan TA, Birey F, Caneda C, Reimer R, Quake SR, Barres BA, Paşca SP
Neuron (2017) 954: 779-790.e6. 160 011 ICC; tested species: human
Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice.
Hayashi S, Inoue Y, Hattori S, Kaneko M, Shioi G, Miyakawa T, Takeichi M
Scientific reports (2017) 71: 5801. 160 011 ICC; tested species: mouse
Posttranslational modification impact on the mechanism by which amyloid-β induces synaptic dysfunction.
Grochowska KM, Yuanxiang P, Bär J, Raman R, Brugal G, Sahu G, Schweizer M, Bikbaev A, Schilling S, Demuth HU, Kreutz MR, et al.
EMBO reports (2017) 186: 962-981. 160 011 ICC; tested species: rat
Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 160 011 ICC
Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse.
Tagliatti E, Fadda M, Falace A, Benfenati F, Fassio A
eLife (2016) 5: . 160 011 ICC; tested species: rat
Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo.
Ivanova D, Dirks A, Montenegro-Venegas C, Schöne C, Altrock WD, Marini C, Frischknecht R, Schanze D, Zenker M, Gundelfinger ED, Fejtova A, et al.
The EMBO journal (2015) 348: 1056-77. 160 011 ICC
The RhoGEF DOCK10 is essential for dendritic spine morphogenesis.
Jaudon F, Raynaud F, Wehrlé R, Bellanger JM, Doulazmi M, Vodjdani G, Gasman S, Fagni L, Dusart I, Debant A, Schmidt S, et al.
Molecular biology of the cell (2015) 2611: 2112-27. 160 011 ICC
NMDA-induced accumulation of Shank at the postsynaptic density is mediated by CaMKII.
Tao-Cheng JH, Yang Y, Bayer KU, Reese TS, Dosemeci A
Biochemical and biophysical research communications (2014) 4501: 808-11. 160 011 ICC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 160 011 ICC
Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription ofArc/Arg3.1and Other Immediate Early Genes.
Dunn CJ, Sarkar P, Bailey ER, Farris S, Zhao M, Ward JM, Dudek SM, Saha RN
eNeuro () 44: . 160 011 ICC; tested species: rat
Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities.
Tao-Cheng JH, Azzam R, Crocker V, Winters CA, Reese T
eNeuro () 26: . 160 011 ICC
Superresolution fluorescence microscopy for 3D reconstruction of thick samples.
Park S, Kang W, Kwon YD, Shim J, Kim S, Kaang BK, Hohng S
Molecular brain (2018) 111: 17. 160 011 IHC; tested species: mouse
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 160 011 IHC; tested species: mouse
Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid.
Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A
Communications biology (2018) 1: 114. 160 011 EM; tested species: rat
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 011 WB, EM
Cat. No.: 160 011
Quantity: 100 µg
Price: $425.00
Rho Guanine Nucleotide Exchange Factor 4 (Arhgef4) Deficiency Enhances Spatial and Object Recognition Memory.
Yoo KS, Lee K, Lee YS, Oh WJ, Kim HK
Experimental neurobiology (2020) 295: 334-343. 160 011 WB; tested species: mouse
Glutamate treatment mimics LTP- and LTD-like biochemical activity in viable synaptosome preparation.
Gharami K, Biswas SC
Neurochemistry international (2019) 134: 104655. 160 011 WB; tested species: mouse
JIP2 haploinsufficiency contributes to neurodevelopmental abnormalities in human pluripotent stem cell-derived neural progenitors and cortical neurons.
Roessler R, Goldmann J, Shivalila C, Jaenisch R
Life science alliance (2018) 14: e201800094. 160 011 WB; tested species: human
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 011 WB, EM
Neuroinflammation induces synaptic scaling through IL-1β-mediated activation of the transcriptional repressor REST/NRSF.
Buffolo F, Petrosino V, Albini M, Moschetta M, Carlini F, Floss T, Kerlero de Rosbo N, Cesca F, Rocchi A, Uccelli A, Benfenati F, et al.
Cell death & disease (2021) 122: 180. 160 011 ICC; tested species: mouse
A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids.
Renner H, Grabos M, Becker KJ, Kagermeier TE, Wu J, Otto M, Peischard S, Zeuschner D, TsyTsyura Y, Disse P, Klingauf J, et al.
eLife (2020) 9: . 160 011 ICC; tested species: human
Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F
Nanoscale (2020) 1218: 10226-10239. 160 011 ICC; tested species: rat
Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation.
Zhang K, Yu F, Zhu J, Han S, Chen J, Wu X, Chen Y, Shen T, Liao J, Guo W, Yang X, et al.
Cell reports (2020) 313: 107521. 160 011 ICC; tested species: human
Acute knockdown of Depdc5 leads to synaptic defects in mTOR-related epileptogenesis.
De Fusco A, Cerullo MS, Marte A, Michetti C, Romei A, Castroflorio E, Baulac S, Benfenati F
Neurobiology of disease (2020) : 104822. 160 011 ICC; tested species: mouse
Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses.
Mitlöhner J, Kaushik R, Niekisch H, Blondiaux A, Gee CE, Happel MFK, Gundelfinger E, Dityatev A, Frischknecht R, Seidenbecher C
Cells (2020) 92: . 160 011 ICC; tested species: rat
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 160 011 ICC; tested species: mouse
The RAB3-RIM Pathway Is Essential for the Release of Neuromodulators.
Persoon CM, Hoogstraaten RI, Nassal JP, van Weering JRT, Kaeser PS, Toonen RF, Verhage M
Neuron (2019) 1046: 1065-1080.e12. 160 011 ICC; tested species: mouse
Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course.
Esposito A, Falace A, Wagner M, Gal M, Mei D, Conti V, Pisano T, Aprile D, Cerullo MS, De Fusco A, Giovedì S, et al.
Brain : a journal of neurology (2019) : . 160 011 ICC; tested species: mouse
De novo mutations of the ATP6V1A gene cause developmental encephalopathy with epilepsy.
Fassio A, Esposito A, Kato M, Saitsu H, Mei D, Marini C, Conti V, Nakashima M, Okamoto N, Olmez Turker A, Albuz B, et al.
Brain : a journal of neurology (2018) 1416: 1703-1718. 160 011 ICC; tested species: rat
Constitutive Inactivation of the PRRT2 Gene Alters Short-Term Synaptic Plasticity and Promotes Network Hyperexcitability in Hippocampal Neurons.
Valente P, Romei A, Fadda M, Sterlini B, Lonardoni D, Forte N, Fruscione F, Castroflorio E, Michetti C, Giansante G, Valtorta F, et al.
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 160 011 ICC; tested species: mouse
Adenomatous polyposis coli-stimulated GEF 1 (Asef1) is a negative regulator of excitatory synaptic function.
Oh JY, Lim CS, Yoo KS, Park HJ, Park YS, Kim EG, Lee YS, Kaang BK, Kim HK
Journal of neurochemistry (2018) : . 160 011 ICC; tested species: rat
Systematic Three-Dimensional Coculture Rapidly Recapitulates Interactions between Human Neurons and Astrocytes.
Krencik R, Seo K, van Asperen JV, Basu N, Cvetkovic C, Barlas S, Chen R, Ludwig C, Wang C, Ward ME, Gan L, et al.
Stem cell reports (2017) 96: 1745-1753. 160 011 ICC
Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 160 011 ICC; tested species: mouse
Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.
Sloan SA, Darmanis S, Huber N, Khan TA, Birey F, Caneda C, Reimer R, Quake SR, Barres BA, Paşca SP
Neuron (2017) 954: 779-790.e6. 160 011 ICC; tested species: human
Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice.
Hayashi S, Inoue Y, Hattori S, Kaneko M, Shioi G, Miyakawa T, Takeichi M
Scientific reports (2017) 71: 5801. 160 011 ICC; tested species: mouse
Posttranslational modification impact on the mechanism by which amyloid-β induces synaptic dysfunction.
Grochowska KM, Yuanxiang P, Bär J, Raman R, Brugal G, Sahu G, Schweizer M, Bikbaev A, Schilling S, Demuth HU, Kreutz MR, et al.
EMBO reports (2017) 186: 962-981. 160 011 ICC; tested species: rat
Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 160 011 ICC
Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse.
Tagliatti E, Fadda M, Falace A, Benfenati F, Fassio A
eLife (2016) 5: . 160 011 ICC; tested species: rat
Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo.
Ivanova D, Dirks A, Montenegro-Venegas C, Schöne C, Altrock WD, Marini C, Frischknecht R, Schanze D, Zenker M, Gundelfinger ED, Fejtova A, et al.
The EMBO journal (2015) 348: 1056-77. 160 011 ICC
The RhoGEF DOCK10 is essential for dendritic spine morphogenesis.
Jaudon F, Raynaud F, Wehrlé R, Bellanger JM, Doulazmi M, Vodjdani G, Gasman S, Fagni L, Dusart I, Debant A, Schmidt S, et al.
Molecular biology of the cell (2015) 2611: 2112-27. 160 011 ICC
NMDA-induced accumulation of Shank at the postsynaptic density is mediated by CaMKII.
Tao-Cheng JH, Yang Y, Bayer KU, Reese TS, Dosemeci A
Biochemical and biophysical research communications (2014) 4501: 808-11. 160 011 ICC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 160 011 ICC
Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription ofArc/Arg3.1and Other Immediate Early Genes.
Dunn CJ, Sarkar P, Bailey ER, Farris S, Zhao M, Ward JM, Dudek SM, Saha RN
eNeuro () 44: . 160 011 ICC; tested species: rat
Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities.
Tao-Cheng JH, Azzam R, Crocker V, Winters CA, Reese T
eNeuro () 26: . 160 011 ICC
Superresolution fluorescence microscopy for 3D reconstruction of thick samples.
Park S, Kang W, Kwon YD, Shim J, Kim S, Kaang BK, Hohng S
Molecular brain (2018) 111: 17. 160 011 IHC; tested species: mouse
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 160 011 IHC; tested species: mouse
Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid.
Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A
Communications biology (2018) 1: 114. 160 011 EM; tested species: rat
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 011 WB, EM
Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.