|Cat. No. 160 002||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery
IHC-P/FFPE: 1 : 500 gallery
|Immunogen||Recombinant protein corresponding to AA 1 to 196 from human Homer1 (UniProt Id: Q86YM7)|
Reacts with: human (Q86YM7), rat (Q9Z214), mouse (Q9Z2Y3).
Other species not tested yet.
Specific for Homer 1. Cross-reactivity of the serum to Homer 2 and 3 was removed by pre-adsorption with Homer 2 (aa 1 - 176) and Homer 3 (aa 1 - 177).
According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.
|Matching control protein/peptide||160-0P|
ICC: PFA fixation is recommended.
Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.