Synaptic Systems

Cat. No.: 160 011

Amount: 100 µg

Price: $430.00

Cat. No. 160 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: not recommended IHC-P: not recommended ExM: yes (see remarks) gallery DNA-PAINT: yes EM: yes ELISA: yes (see remarks)
Clone	2G8
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to the N-terminal half of human Homer 1 (UniProt Id: Q86YM7)
Reactivity	Reacts with: human (Q86YM7), rat (Q9Z214), mouse (Q9Z2Y3). Other species not tested yet.
Specificity	Specific for Homer 1. According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.
Matching control protein/peptide	160-0P
Remarks	ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453). ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 160 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	160_011.pdf

References for Homer1 - 160 011

Synaptic neoteny of human cortical neurons requires species-specific balancing of SRGAP2-SYNGAP1 cross-inhibition.
Libé-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, Gaspariunaite V, et al.
Neuron (2024) : . 160 011 WB; tested species: human

The kinesin Kif21b binds myosin Va and mediates changes in actin dynamics underlying homeostatic synaptic downscaling.
Gromova KV, Thies E, Janiesch PC, Lützenkirchen FP, Zhu Y, Stajano D, Dürst CD, Schweizer M, Konietzny A, Mikhaylova M, Gee CE, et al.
Cell reports (2023) 427: 112743. 160 011 WB; tested species: mouse

SLC13A5/sodium-citrate co-transporter overexpression causes disrupted white matter integrity and an autistic-like phenotype.
Rigby MJ, Orefice NS, Lawton AJ, Ma M, Shapiro SL, Yi SY, Dieterich IA, Frelka A, Miles HN, Pearce RA, Yu JPJ, et al.
Brain communications (2022) 41: fcac002. 160 011 WB, ICC; tested species: mouse

Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 160 011 WB, ICC; tested species: mouse

Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.
Baldassari S, Cervetto C, Amato S, Fruscione F, Balagura G, Pelassa S, Musante I, Iacomino M, Traverso M, Corradi A, Scudieri P, et al.
International journal of molecular sciences (2022) 2318: . 160 011 WB, ICC; tested species: human

Tubb3 expression levels are sensitive to neuronal activity changes and determine microtubule growth and kinesin-mediated transport.
Radwitz J, Hausrat TJ, Heisler FF, Janiesch PC, Pechmann Y, Rübhausen M, Kneussel M
Cellular and molecular life sciences : CMLS (2022) 7911: 575. 160 011 WB; tested species: mouse

Rho Guanine Nucleotide Exchange Factor 4 (Arhgef4) Deficiency Enhances Spatial and Object Recognition Memory.
Yoo KS, Lee K, Lee YS, Oh WJ, Kim HK
Experimental neurobiology (2020) 295: 334-343. 160 011 WB; tested species: mouse

Glutamate treatment mimics LTP- and LTD-like biochemical activity in viable synaptosome preparation.
Gharami K, Biswas SC
Neurochemistry international (2019) 134: 104655. 160 011 WB; tested species: mouse

JIP2 haploinsufficiency contributes to neurodevelopmental abnormalities in human pluripotent stem cell-derived neural progenitors and cortical neurons.
Roessler R, Goldmann J, Shivalila C, Jaenisch R
Life science alliance (2018) 14: e201800094. 160 011 WB; tested species: human

Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 011 WB, EM

Glial Cx43 hemichannels and neuronal Panx1 hemichannels and P2X7 receptors orchestrates presynaptic homeostatic plasticity.
Rafael A, Tizzoni M, Justet C, Cairus A, Abudara V, Vitureira N
Cellular signalling (2024) : 111113. 160 011 ICC; tested species: rat

Real-time visualization of structural dynamics of synapses in live cells in vivo.
Son S, Nagahama K, Lee J, Jung K, Kwak C, Kim J, Noh YW, Kim E, Lee S, Kwon HB, Heo WD, et al.
Nature methods (2024) : . 160 011 ICC; tested species: rat

ANK2 loss-of-function variants are associated with epilepsy, and lead to impaired axon initial segment plasticity and hyperactive network activity in hiPSC-derived neuronal networks.
Teunissen MWA, Lewerissa E, van Hugte EJH, Wang S, Ockeloen CW, Koolen DA, Pfundt R, Marcelis CLM, Brilstra E, Howe JL, Scherer SW, et al.
Human molecular genetics (2023) 3214: 2373-2385. 160 011 ICC; tested species: human

An in silico and in vitro human neuronal network model reveals cellular mechanisms beyond NaV1.1 underlying Dravet syndrome.
Doorn N, van Hugte EJH, Ciptasari U, Mordelt A, Meijer HGE, Schubert D, Frega M, Nadif Kasri N, van Putten MJAM
Stem cell reports (2023) 188: 1686-1700. 160 011 ICC; tested species: human

Generation of glutamatergic/GABAergic neuronal co-cultures derived from human induced pluripotent stem cells for characterizing E/I balance in vitro.
Wang S, Hesen R, Mossink B, Nadif Kasri N, Schubert D
STAR protocols (2023) 41: 101967. 160 011 ICC; tested species: human

Effects of Tropomodulin 2 on Dendritic Spine Reorganization and Dynamics.
Kuruba B, Starks N, Josten MR, Naveh O, Wayman G, Mikhaylova M, Kostyukova AS
Biomolecules (2023) 138: . 160 011 ICC; tested species: rat

Sialic acid biosynthesis pathway blockade disturbs neuronal network formation in human iPSC-derived excitatory neurons.
Mijdam R, Bijnagte-Schoenmaker C, Dyke E, Moons SJ, Boltje TJ, Nadif Kasri N, Lefeber DJ
Journal of neurochemistry (2023) : . 160 011 ICC; tested species: human

Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation.
Sun J, Osenberg S, Irwin A, Ma LH, Lee N, Xiang Y, Li F, Wan YW, Park IH, Maletic-Savatic M, Ballas N, et al.
Cell reports (2023) 421: 111942. 160 011 ICC, IHC; tested species: human

RNA supply drives physiological granule assembly in neurons.
Bauer KE, Bargenda N, Schieweck R, Illig C, Segura I, Harner M, Kiebler MA
Nature communications (2022) 131: 2781. 160 011 ICC; tested species: rat

Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 160 011 ICC; tested species: rat

Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines.
Konietzny A, Grendel J, Kadek A, Bucher M, Han Y, Hertrich N, Dekkers DHW, Demmers JAA, Grünewald K, Uetrecht C, Mikhaylova M, et al.
The EMBO journal (2022) 414: e106523. 160 011 ICC; tested species: mouse,rat

Neuroinflammation induces synaptic scaling through IL-1β-mediated activation of the transcriptional repressor REST/NRSF.
Buffolo F, Petrosino V, Albini M, Moschetta M, Carlini F, Floss T, Kerlero de Rosbo N, Cesca F, Rocchi A, Uccelli A, Benfenati F, et al.
Cell death & disease (2021) 122: 180. 160 011 ICC; tested species: mouse

Imbalanced autophagy causes synaptic deficits in a human model for neurodevelopmental disorders.
Linda K, Lewerissa EI, Verboven AHA, Gabriele M, Frega M, Klein Gunnewiek TM, Devilee L, Ulferts E, Hommersom M, Oudakker A, Schoenmaker C, et al.
Autophagy (2021) : 1-20. 160 011 ICC; tested species: human

Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons.
Sertel SM, Blumenstein W, Mandad S, Shomroni O, Salinas G, Rizzoli SO
Scientific reports (2021) 111: 17374. 160 011 ICC; tested species: rat

Acute knockdown of Depdc5 leads to synaptic defects in mTOR-related epileptogenesis.
De Fusco A, Cerullo MS, Marte A, Michetti C, Romei A, Castroflorio E, Baulac S, Benfenati F
Neurobiology of disease (2020) : 104822. 160 011 ICC; tested species: mouse

Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses.
Mitlöhner J, Kaushik R, Niekisch H, Blondiaux A, Gee CE, Happel MFK, Gundelfinger E, Dityatev A, Frischknecht R, Seidenbecher C
Cells (2020) 92: . 160 011 ICC; tested species: rat

Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation.
Zhang K, Yu F, Zhu J, Han S, Chen J, Wu X, Chen Y, Shen T, Liao J, Guo W, Yang X, et al.
Cell reports (2020) 313: 107521. 160 011 ICC; tested species: human

Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.
Bayraktar G, Yuanxiang P, Confettura AD, Gomes GM, Raza SA, Stork O, Tajima S, Suetake I, Karpova A, Yildirim F, Kreutz MR, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2020) 4512: 2120-2130. 160 011 ICC; tested species: rat

Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F
Nanoscale (2020) 1218: 10226-10239. 160 011 ICC; tested species: rat

A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids.
Renner H, Grabos M, Becker KJ, Kagermeier TE, Wu J, Otto M, Peischard S, Zeuschner D, TsyTsyura Y, Disse P, Klingauf J, et al.
eLife (2020) 9: . 160 011 ICC; tested species: human

The RAB3-RIM Pathway Is Essential for the Release of Neuromodulators.
Persoon CM, Hoogstraaten RI, Nassal JP, van Weering JRT, Kaeser PS, Toonen RF, Verhage M
Neuron (2019) 1046: 1065-1080.e12. 160 011 ICC; tested species: mouse

Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course.
Esposito A, Falace A, Wagner M, Gal M, Mei D, Conti V, Pisano T, Aprile D, Cerullo MS, De Fusco A, Giovedì S, et al.
Brain : a journal of neurology (2019) : . 160 011 ICC; tested species: mouse

Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 160 011 ICC; tested species: mouse

De novo mutations of the ATP6V1A gene cause developmental encephalopathy with epilepsy.
Fassio A, Esposito A, Kato M, Saitsu H, Mei D, Marini C, Conti V, Nakashima M, Okamoto N, Olmez Turker A, Albuz B, et al.
Brain : a journal of neurology (2018) 1416: 1703-1718. 160 011 ICC; tested species: rat

Constitutive Inactivation of the PRRT2 Gene Alters Short-Term Synaptic Plasticity and Promotes Network Hyperexcitability in Hippocampal Neurons.
Valente P, Romei A, Fadda M, Sterlini B, Lonardoni D, Forte N, Fruscione F, Castroflorio E, Michetti C, Giansante G, Valtorta F, et al.
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 160 011 ICC; tested species: mouse

Adenomatous polyposis coli-stimulated GEF 1 (Asef1) is a negative regulator of excitatory synaptic function.
Oh JY, Lim CS, Yoo KS, Park HJ, Park YS, Kim EG, Lee YS, Kaang BK, Kim HK
Journal of neurochemistry (2018) : . 160 011 ICC; tested species: rat

Posttranslational modification impact on the mechanism by which amyloid-β induces synaptic dysfunction.
Grochowska KM, Yuanxiang P, Bär J, Raman R, Brugal G, Sahu G, Schweizer M, Bikbaev A, Schilling S, Demuth HU, Kreutz MR, et al.
EMBO reports (2017) 186: 962-981. 160 011 ICC; tested species: rat

Systematic Three-Dimensional Coculture Rapidly Recapitulates Interactions between Human Neurons and Astrocytes.
Krencik R, Seo K, van Asperen JV, Basu N, Cvetkovic C, Barlas S, Chen R, Ludwig C, Wang C, Ward ME, Gan L, et al.
Stem cell reports (2017) 96: 1745-1753. 160 011 ICC

Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 160 011 ICC; tested species: mouse

Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.
Sloan SA, Darmanis S, Huber N, Khan TA, Birey F, Caneda C, Reimer R, Quake SR, Barres BA, Paşca SP
Neuron (2017) 954: 779-790.e6. 160 011 ICC; tested species: human

Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice.
Hayashi S, Inoue Y, Hattori S, Kaneko M, Shioi G, Miyakawa T, Takeichi M
Scientific reports (2017) 71: 5801. 160 011 ICC; tested species: mouse

Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy.
Altmüller F, Pothula S, Annamneedi A, Nakhaei-Rad S, Montenegro-Venegas C, Pina-Fernández E, Marini C, Santos M, Schanze D, Montag D, Ahmadian MR, et al.
PLoS genetics (2017) 133: e1006684. 160 011 ICC

Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse.
Tagliatti E, Fadda M, Falace A, Benfenati F, Fassio A
eLife (2016) 5: . 160 011 ICC; tested species: rat

Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo.
Ivanova D, Dirks A, Montenegro-Venegas C, Schöne C, Altrock WD, Marini C, Frischknecht R, Schanze D, Zenker M, Gundelfinger ED, Fejtova A, et al.
The EMBO journal (2015) 348: 1056-77. 160 011 ICC

The RhoGEF DOCK10 is essential for dendritic spine morphogenesis.
Jaudon F, Raynaud F, Wehrlé R, Bellanger JM, Doulazmi M, Vodjdani G, Gasman S, Fagni L, Dusart I, Debant A, Schmidt S, et al.
Molecular biology of the cell (2015) 2611: 2112-27. 160 011 ICC

NMDA-induced accumulation of Shank at the postsynaptic density is mediated by CaMKII.
Tao-Cheng JH, Yang Y, Bayer KU, Reese TS, Dosemeci A
Biochemical and biophysical research communications (2014) 4501: 808-11. 160 011 ICC

Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 160 011 ICC

Histone Hypervariants H2A.Z.1 and H2A.Z.2 Play Independent and Context-Specific Roles in Neuronal Activity-Induced Transcription ofArc/Arg3.1and Other Immediate Early Genes.
Dunn CJ, Sarkar P, Bailey ER, Farris S, Zhao M, Ward JM, Dudek SM, Saha RN
eNeuro () 44: . 160 011 ICC; tested species: rat

Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities.
Tao-Cheng JH, Azzam R, Crocker V, Winters CA, Reese T
eNeuro () 26: . 160 011 ICC

Synaptic cell-adhesion molecule latrophilin-2 is differentially directed to dendritic domains of hippocampal neurons.
Murphy TR, Amidon RF, Donohue JD, Li L, Anderson GR
iScience (2024) 272: 108799. 160 011 IHC; tested species: mouse

Teneurins assemble into presynaptic nanoclusters that promote synapse formation via postsynaptic non-teneurin ligands.
Zhang X, Lin PY, Liakath-Ali K, Südhof TC
Nature communications (2022) 131: 2297. 160 011 IHC; tested species: mouse

ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids.
Bowles KR, Silva MC, Whitney K, Bertucci T, Berlind JE, Lai JD, Garza JC, Boles NC, Mahali S, Strang KH, Marsh JA, et al.
Cell (2021) 18417: 4547-4563.e17. 160 011 IHC; tested species: human

Superresolution fluorescence microscopy for 3D reconstruction of thick samples.
Park S, Kang W, Kwon YD, Shim J, Kim S, Kaang BK, Hohng S
Molecular brain (2018) 111: 17. 160 011 IHC; tested species: mouse

Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 160 011 IHC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 160 011 DNA-PAINT; tested species: rat

A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging.
Falkovich R, Danielson EW, Perez de Arce K, Wamhoff EC, Strother J, Lapteva AP, Sheng M, Cottrell JR, Bathe M
Cell reports (2023) 425: 112430. 160 011 DNA-PAINT; tested species: rat

Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid.
Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A
Communications biology (2018) 1: 114. 160 011 EM; tested species: rat

Cat. No.: 160 011

Amount: 100 µg

Price: $430.00

ICC

ExM

Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.

1 ) Kammermeier PJ et al., BMC Neurosci (2006)
2 ) Yamamoto K et al., Eur. J. Neurosci. (2005)
3 ) Thomas U et al., J. Neurochem. (2002)
4 ) Ango F et al., Mol. Cell. Neurosci. (2002)
5 ) Sala C et al., Neuron (2001)

Protocols

Standard protocol

ICC

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Homer1 antibody - 160 011

References for Homer1 - 160 011