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anti-Chicken IgY sdAb - FluoTag-X2 - N0702-AF-S

Secondary single domain antibody specific for chicken IgY

 

This product was developed by   

NanoTag-Biotechnologies

FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Single Domain

Cat. No.: N0702-AF-S
Amount: 500 µl
Price: $195.00
Cat. No. N0702-AF-S

500µl 5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide

Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Applications ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
Label AcidiFluor Orange, two fluorophores coupled to one FluoTag
Clone 4B1
Subtype single domain
Reactivity Reacts with: chicken.
No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Recognizes native chicken IgY. It does not cross-react to mouse, rabbit, Guinea pig, rat, donkey, goat, pig, horse, cow or human IgGs.
Documents data sheet
Remarks

ICC: This antibody is intended to be used for uptake experiments in cell cultures. Primary antibodies against extracellular epitopes can be pre-labeled with this FluoTag. The pH sensitive dye regaines its fluorescence after the reacidification of the intracellular compartment.


Cat. No.: N0702-AF-S
Quantity: 500 µl
Price: $195.00
Background

IgY is the major immunoglobulin found in chicken eggs. FluoTag®-X2 anti-chicken is a species-specific FluoTag®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.