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mCLING-ATTO 488-labeled - 710 006AT3

A fixable membrane probe for high resolution microscopy
Cat. No.: 710 006AT3
Amount: 5 nmol
Price: 330.00 €
Cat. No. 710 006AT3

5nmol mCLING labeled with ATTO® 488 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.

 

Applications ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml)
Label ATTO 488
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Data sheet 710_006at3.pdf

References for mCLING-ATTO 488-labeled - 710 006AT3

A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT3 ICC, FACS
Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis.
Shin W, Ge L, Arpino G, Villarreal SA, Hamid E, Liu H, Zhao WD, Wen PJ, Chiang HC, Wu LG
Cell (2018) : . 710 006AT3 ICC
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT3 ICC, FACS
Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish.
Manchanda A, Chatterjee P, Bonventre JA, Haggard DE, Kindt KS, Tanguay RL, Johnson CP
Scientific reports (2019) 91: 14273. 710 006AT3 UPTAKE; tested species: zebrafish
Cat. No.: 710 006AT3
Quantity: 5 nmol
Price: 330.00 €
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT3 ICC, FACS
Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis.
Shin W, Ge L, Arpino G, Villarreal SA, Hamid E, Liu H, Zhao WD, Wen PJ, Chiang HC, Wu LG
Cell (2018) : . 710 006AT3 ICC
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT3 ICC, FACS
Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish.
Manchanda A, Chatterjee P, Bonventre JA, Haggard DE, Kindt KS, Tanguay RL, Johnson CP
Scientific reports (2019) 91: 14273. 710 006AT3 UPTAKE; tested species: zebrafish
Background
The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.