Home|||||||Technical Support|

mCLING-ATTO 647N-labeled - 710 006AT1

A fixable membrane probe for high resolution microscopy
Cat. No.: 710 006AT1
Amount: 5 nmol
Price: 330.00 €
Cat. No. 710 006AT1

5nmol mCLING labeled with ATTO® 647N in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.

Applications ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml)
Label ATTO 647N
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Data sheet 710_006at1.pdf

References for mCLING-ATTO 647N-labeled - 710 006AT1

Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells.
Zila V, Müller TG, Laketa V, Müller B, Kräusslich HG
mBio (2019) 106: . 710 006AT1 ICC; tested species: human
Reversible supramolecular assembly of velvet worm adhesive fibers via electrostatic interactions of charged phosphoproteins.
Baer A, Hänsch S, Mayer G, Harrington MJ, Schmidt S
Biomacromolecules (2018) : . 710 006AT1 ICC
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA, Lind AL, Smith SE, Li R, Gould KL
eLife (2017) 6: . 710 006AT1 ICC
An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells.
Taniguchi K, Shao Y, Townshend RF, Cortez CL, Harris CE, Meshinchi S, Kalantry S, Fu J, O'Shea KS, Gumucio DL
The Journal of cell biology (2017) 21612: 3981-3990. 710 006AT1 ICC; tested species: human
Mechanoresponsive lipid-protein nanoglobules facilitate reversible fibre formation in velvet worm slime.
Baer A, Schmidt S, Haensch S, Eder M, Mayer G, Harrington MJ
Nature communications (2017) 81: 974. 710 006AT1 ICC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT1 ICC, IHC
SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis.
Baranov MV, Revelo NH, Dingjan I, Maraspini R, Ter Beest M, Honigmann A, van den Bogaart G
Cell reports (2016) 176: 1518-1531. 710 006AT1 ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT1 ICC, IHC
Ma2/d promotes myonuclear positioning and association with the sarcoplasmic reticulum.
Reuveny A, Shnayder M, Lorber D, Wang S, Volk T
Development (Cambridge, England) (2018) 14517: . 710 006AT1 IHC; tested species: drosophila
Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii.
Chartier TF, Deschamps J, Dürichen W, Jékely G, Arendt D
Open biology (2018) 810: . 710 006AT1 IHC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT1 ICC, IHC
Disruption of adaptor protein 2μ (AP-2μ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung S, Maritzen T, Wichmann C, Jing Z, Neef A, Revelo NH, Al-Moyed H, Meese S, Wojcik SM, Panou I, Bulut H, et al.
The EMBO journal (2015) 3421: 2686-702. 710 006AT1 IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT1 ICC, IHC
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 710 006AT1 UPTAKE; tested species: mouse
Cat. No.: 710 006AT1
Quantity: 5 nmol
Price: 330.00 €
Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells.
Zila V, Müller TG, Laketa V, Müller B, Kräusslich HG
mBio (2019) 106: . 710 006AT1 ICC; tested species: human
Reversible supramolecular assembly of velvet worm adhesive fibers via electrostatic interactions of charged phosphoproteins.
Baer A, Hänsch S, Mayer G, Harrington MJ, Schmidt S
Biomacromolecules (2018) : . 710 006AT1 ICC
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA, Lind AL, Smith SE, Li R, Gould KL
eLife (2017) 6: . 710 006AT1 ICC
An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells.
Taniguchi K, Shao Y, Townshend RF, Cortez CL, Harris CE, Meshinchi S, Kalantry S, Fu J, O'Shea KS, Gumucio DL
The Journal of cell biology (2017) 21612: 3981-3990. 710 006AT1 ICC; tested species: human
Mechanoresponsive lipid-protein nanoglobules facilitate reversible fibre formation in velvet worm slime.
Baer A, Schmidt S, Haensch S, Eder M, Mayer G, Harrington MJ
Nature communications (2017) 81: 974. 710 006AT1 ICC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT1 ICC, IHC
SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis.
Baranov MV, Revelo NH, Dingjan I, Maraspini R, Ter Beest M, Honigmann A, van den Bogaart G
Cell reports (2016) 176: 1518-1531. 710 006AT1 ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT1 ICC, IHC
Ma2/d promotes myonuclear positioning and association with the sarcoplasmic reticulum.
Reuveny A, Shnayder M, Lorber D, Wang S, Volk T
Development (Cambridge, England) (2018) 14517: . 710 006AT1 IHC; tested species: drosophila
Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii.
Chartier TF, Deschamps J, Dürichen W, Jékely G, Arendt D
Open biology (2018) 810: . 710 006AT1 IHC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT1 ICC, IHC
Disruption of adaptor protein 2μ (AP-2μ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung S, Maritzen T, Wichmann C, Jing Z, Neef A, Revelo NH, Al-Moyed H, Meese S, Wojcik SM, Panou I, Bulut H, et al.
The EMBO journal (2015) 3421: 2686-702. 710 006AT1 IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT1 ICC, IHC
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 710 006AT1 UPTAKE; tested species: mouse
Background
The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.