|Cat. No. 226-0P||
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Control peptides should be stored at -20°C when still lyophilized!
|Immunogen||Synthetic peptide corresponding to AA 2 to 17 from rat c-Fos (UniProt Id: P12841)|
|Recommended dilution||Optimal concentrations should be determined by the end-user.|
|Matching antibodies||226 003, 226 004, 226 005, 226 013, 226 017|
This control peptide consists of the synthetic peptide (aa 2 - 17 of rat c-Fos) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).