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c-Fos - 226 017

A member of the transcription factor complex AP1
Monoclonal rat purified IgG
Cat. No.: 226 017
Amount: 100 µg
Price: 415.00 €
Cat. No. 226 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 200 gallery  
Clone 108B5H5
Immunogen Synthetic peptide corresponding to AA 2 to 17 from rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Specificity Specific for c-Fos
Matching control protein/peptide 226-0P
Remarks

IHC: Signal quality is strongly enhanced when antibody is incubated at RT.

Data sheet 226_017.pdf
Cat. No.: 226 017
Quantity: 100 µg
Price: 415.00 €
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).