|Cat. No. 226 017||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery
IHC-P/FFPE: 1 : 100 up to 1 : 200 gallery
FACS: yes gallery
|Subtype||IgG2a (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 2 to 17 from rat c-Fos (UniProt Id: P12841)|
Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
|Specificity||Specific for c-Fos|
|Matching control protein/peptide||226-0P|
IHC: For best results tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.
The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).