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c-Fos antibody - 226 017

c-Fos is a member of the transcription factor complex AP1
Rat monoclonal purified IgG
Cat. No.: 226 017
Amount: 100 µg
Price: $425.00
Cat. No. 226 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 200 gallery  
FACS: yes gallery  
Clone 108B5H5
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Matching control protein/peptide 226-0P
Remarks

IHC: For best results tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.

Data sheet 226_017.pdf

References for c-Fos - 226 017

Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
Hippocampus-to-amygdala pathway drives the separation of remote memories of related events.
Concina G, Milano L, Renna A, Manassero E, Stabile F, Sacchetti B
Cell reports (2024) 435: 114151. 226 017 IHC; tested species: rat
Stimulating intestinal GIP release reduces food intake and body weight in mice.
Lewis JE, Nuzzaci D, James-Okoro PP, Montaner M, O'Flaherty Rottenberger E, Darwish T, Hayashi M, Liberles SD, Hornigold D, Naylor J, Baker D, et al.
Molecular metabolism (2024) : 101945. 226 017 IHC; tested species: mouse
A multiple Arc (mArc) tagging system to uncover the organizational principles of multiple memories.
Stackmann M, Yelhekar TD, Meng M, Sun X, Nthumba J, Bulthuis NE, Vaughan N, Zhu E, Lin Y, Denny CA
bioRxiv : the preprint server for biology (2024) : . 226 017 IHC; tested species: mouse
Astrocytes in preoptic area regulate acute nociception-induced hypothermia through adenosine receptors.
Jia J, Chen T, Chen C, Si T, Gao C, Fang Y, Sun J, Wang J, Zhang Z
CNS neuroscience & therapeutics (2024) 305: e14726. 226 017 IHC; tested species: mouse
Cry1Δ11 mutation induces ADHD-like symptoms through hyperactive dopamine D1 receptor signaling.
Liu D, Xie Z, Gu P, Li X, Zhang Y, Wang X, Chen Z, Deng S, Shu Y, Li JD
JCI insight (2023) 816: . 226 017 IHC; tested species: mouse
Adrenergic signalling to astrocytes in anterior cingulate cortex contributes to pain-related aversive memory in rats.
Iqbal Z, Lei Z, Ramkrishnan AS, Liu S, Hasan M, Akter M, Lam YY, Li Y
Communications biology (2023) 61: 10. 226 017 IHC; tested species: rat
Chronic Pb Exposure Induces Anxiety and Depression-like Behaviors in Mice via Excitatory Neuronal Hyperexcitability in Ventral Hippocampal Dentate Gyrus.
Zhou R, Huang C, Bi N, Li L, Li C, Gu X, Song Y, Wang HL
Environmental science & technology (2023) 5733: 12222-12233. 226 017 IHC; tested species: mouse
Bidirectional Regulation of Motor Circuits Using Magnetogenetic Gene Therapy.
Unda SR, Marongiu R, Pomeranz LE, Dyke JP, Fung EK, Grosenick L, Zirkel R, Antoniazzi AM, Norman S, Liston CM, Schaffer C, et al.
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: mouse
Whole-brain analysis of CO2 chemosensitive regions and identification of the retrotrapezoid and medullary raphé nuclei in the common marmoset (Callithrix jacchus).
Turk AZ, Millwater M, SheikhBahaei S
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: marmoset
Prelimbic area to lateral hypothalamus circuit drives social aggression.
Bai F, Huang L, Deng J, Long Z, Hao X, Chen P, Wu G, Wen H, Deng Q, Bao X, Huang J, et al.
iScience (2023) 2610: 107718. 226 017 IHC; tested species: mouse
Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning.
Lamothe-Molina PJ, Franzelin A, Beck L, Li D, Auksutat L, Fieblinger T, Laprell L, Alhbeck J, Gee CE, Kneussel M, Engel AK, et al.
Nature communications (2022) 131: 6376. 226 017 IHC; tested species: mouse
Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
Torromino G, Loffredo V, Cavezza D, Sonsini G, Esposito F, Crevenna AH, Gioffrè M, De Risi M, Treves A, Griguoli M, De Leonibus E, et al.
Nature communications (2022) 131: 4194. 226 017 IHC; tested species: mouse
Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy.
Lentini C, d'Orange M, Marichal N, Trottmann MM, Vignoles R, Foucault L, Verrier C, Massera C, Raineteau O, Conzelmann KK, Rival-Gervier S, et al.
Cell stem cell (2021) : . 226 017 IHC; tested species: mouse
Non-invasive optogenetics with ultrasound-mediated gene delivery and red-light excitation.
Pouliopoulos AN, Murillo MF, Noel RL, Batts AJ, Ji R, Kwon N, Yu H, Tong CK, Gelinas JN, Araghy DK, Hussaini SA, et al.
Brain stimulation () 154: 927-941. 226 017 IHC; tested species: mouse
Cat. No.: 226 017
Amount: 100 µg
Price: $425.00
Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
Hippocampus-to-amygdala pathway drives the separation of remote memories of related events.
Concina G, Milano L, Renna A, Manassero E, Stabile F, Sacchetti B
Cell reports (2024) 435: 114151. 226 017 IHC; tested species: rat
Stimulating intestinal GIP release reduces food intake and body weight in mice.
Lewis JE, Nuzzaci D, James-Okoro PP, Montaner M, O'Flaherty Rottenberger E, Darwish T, Hayashi M, Liberles SD, Hornigold D, Naylor J, Baker D, et al.
Molecular metabolism (2024) : 101945. 226 017 IHC; tested species: mouse
A multiple Arc (mArc) tagging system to uncover the organizational principles of multiple memories.
Stackmann M, Yelhekar TD, Meng M, Sun X, Nthumba J, Bulthuis NE, Vaughan N, Zhu E, Lin Y, Denny CA
bioRxiv : the preprint server for biology (2024) : . 226 017 IHC; tested species: mouse
Astrocytes in preoptic area regulate acute nociception-induced hypothermia through adenosine receptors.
Jia J, Chen T, Chen C, Si T, Gao C, Fang Y, Sun J, Wang J, Zhang Z
CNS neuroscience & therapeutics (2024) 305: e14726. 226 017 IHC; tested species: mouse
Cry1Δ11 mutation induces ADHD-like symptoms through hyperactive dopamine D1 receptor signaling.
Liu D, Xie Z, Gu P, Li X, Zhang Y, Wang X, Chen Z, Deng S, Shu Y, Li JD
JCI insight (2023) 816: . 226 017 IHC; tested species: mouse
Adrenergic signalling to astrocytes in anterior cingulate cortex contributes to pain-related aversive memory in rats.
Iqbal Z, Lei Z, Ramkrishnan AS, Liu S, Hasan M, Akter M, Lam YY, Li Y
Communications biology (2023) 61: 10. 226 017 IHC; tested species: rat
Chronic Pb Exposure Induces Anxiety and Depression-like Behaviors in Mice via Excitatory Neuronal Hyperexcitability in Ventral Hippocampal Dentate Gyrus.
Zhou R, Huang C, Bi N, Li L, Li C, Gu X, Song Y, Wang HL
Environmental science & technology (2023) 5733: 12222-12233. 226 017 IHC; tested species: mouse
Bidirectional Regulation of Motor Circuits Using Magnetogenetic Gene Therapy.
Unda SR, Marongiu R, Pomeranz LE, Dyke JP, Fung EK, Grosenick L, Zirkel R, Antoniazzi AM, Norman S, Liston CM, Schaffer C, et al.
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: mouse
Whole-brain analysis of CO2 chemosensitive regions and identification of the retrotrapezoid and medullary raphé nuclei in the common marmoset (Callithrix jacchus).
Turk AZ, Millwater M, SheikhBahaei S
bioRxiv : the preprint server for biology (2023) : . 226 017 IHC; tested species: marmoset
Prelimbic area to lateral hypothalamus circuit drives social aggression.
Bai F, Huang L, Deng J, Long Z, Hao X, Chen P, Wu G, Wen H, Deng Q, Bao X, Huang J, et al.
iScience (2023) 2610: 107718. 226 017 IHC; tested species: mouse
Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels TL, Blum JA, Gifford DK, Wichterle H
Nature communications (2022) 131: 5427. 226 017 IHC, ICC; tested species: mouse
ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning.
Lamothe-Molina PJ, Franzelin A, Beck L, Li D, Auksutat L, Fieblinger T, Laprell L, Alhbeck J, Gee CE, Kneussel M, Engel AK, et al.
Nature communications (2022) 131: 6376. 226 017 IHC; tested species: mouse
Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
Torromino G, Loffredo V, Cavezza D, Sonsini G, Esposito F, Crevenna AH, Gioffrè M, De Risi M, Treves A, Griguoli M, De Leonibus E, et al.
Nature communications (2022) 131: 4194. 226 017 IHC; tested species: mouse
Reprogramming reactive glia into interneurons reduces chronic seizure activity in a mouse model of mesial temporal lobe epilepsy.
Lentini C, d'Orange M, Marichal N, Trottmann MM, Vignoles R, Foucault L, Verrier C, Massera C, Raineteau O, Conzelmann KK, Rival-Gervier S, et al.
Cell stem cell (2021) : . 226 017 IHC; tested species: mouse
Non-invasive optogenetics with ultrasound-mediated gene delivery and red-light excitation.
Pouliopoulos AN, Murillo MF, Noel RL, Batts AJ, Ji R, Kwon N, Yu H, Tong CK, Gelinas JN, Araghy DK, Hussaini SA, et al.
Brain stimulation () 154: 927-941. 226 017 IHC; tested species: mouse
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).