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c-Fos antibody - 226 009

c-Fos is a member of the transcription factor complex AP1
Chicken monoclonal recombinant IgY
Cat. No.: 226 009
Amount: 50 µg
Price: $415.00
Cat. No. 226 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
ICC: yes gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clone Ch108B5
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Matching control protein/peptide 226-0P

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
IHC: For best results, tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.

Data sheet 226_009.pdf
Cat. No.: 226 009
Amount: 50 µg
Price: $415.00

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).