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Home > Products > 226 308

c-Fos antibody - 226 308

c-Fos is a member of the transcription factor complex AP1
Guinea pig monoclonal recombinant IgG
Cat. No.: 226 308
Amount: 50 µg
Price: $415.00
Cat. No. 226 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
Clone Gp108B5
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Matching control protein/peptide 226-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
IHC: For best results, tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.
Data sheet 226_308.pdf

References for c-Fos - 226 308

Examining the engram encoding specificity hypothesis in mice.
Jung JH, Wang Y, Mocle AJ, Zhang T, Köhler S, Frankland PW, Josselyn SA
Neuron (2023) : . 226 308 IHC; tested species: mouse
Control of non-REM sleep by ventrolateral medulla glutamatergic neurons projecting to the preoptic area.
Teng S, Zhen F, Wang L, Schalchli JC, Simko J, Chen X, Jin H, Makinson CD, Peng Y
Nature communications (2022) 131: 4748. 226 308 IHC; tested species: mouse
Lateral Hypothalamus Calcium/Calmodulin-Dependent Protein Kinase II α Neurons Encode Novelty-Seeking Signals to Promote Predatory Eating.
Tan N, Shi J, Xu L, Zheng Y, Wang X, Lai N, Fang Z, Chen J, Wang Y, Chen Z
Research (Washington, D.C.) (2022) 2022: 9802382. 226 308 IHC; tested species: mouse
Cat. No.: 226 308
Amount: 50 µg
Price: $415.00
Examining the engram encoding specificity hypothesis in mice.
Jung JH, Wang Y, Mocle AJ, Zhang T, Köhler S, Frankland PW, Josselyn SA
Neuron (2023) : . 226 308 IHC; tested species: mouse
Control of non-REM sleep by ventrolateral medulla glutamatergic neurons projecting to the preoptic area.
Teng S, Zhen F, Wang L, Schalchli JC, Simko J, Chen X, Jin H, Makinson CD, Peng Y
Nature communications (2022) 131: 4748. 226 308 IHC; tested species: mouse
Lateral Hypothalamus Calcium/Calmodulin-Dependent Protein Kinase II α Neurons Encode Novelty-Seeking Signals to Promote Predatory Eating.
Tan N, Shi J, Xu L, Zheng Y, Wang X, Lai N, Fang Z, Chen J, Wang Y, Chen Z
Research (Washington, D.C.) (2022) 2022: 9802382. 226 308 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC-P
IHC-P
IHC-P
IHC-P
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).
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