Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

c-Fos antibody - 226 008

c-Fos is a member of the transcription factor complex AP1
Rabbit monoclonal recombinant IgG
Cat. No.: 226 008
Amount: 50 µg
Price: $425.00
Cat. No. 226 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
iDISCO: 1 : 500 gallery  
Clarity: 1 : 10000
Clone Rb108B5
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Matching control protein/peptide 226-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: For best results, tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.

Data sheet 226_008.pdf

References for c-Fos - 226 008

A developmental brain-wide screen identifies retrosplenial cortex as a key player in the emergence of persistent memory.
Jin B, Gongwer MW, Ohanian L, Holden-Wingate L, Le B, Darmawan A, Nakayama Y, Rueda Mora SA, DeNardo LA
bioRxiv : the preprint server for biology (2024) : . 226 008 IHC; tested species: mouse
Circuit mechanisms of navigation strategy learning in mice.
Parrini M, Tricot G, Caroni P, Spolidoro M
Current biology : CB (2024) 341: 79-91.e4. 226 008 IHC; tested species: mouse
A choroid plexus apocrine secretion mechanism shapes CSF proteome and embryonic brain development.
Courtney Y, Head JP, Yimer ED, Dani N, Shipley FB, Libermann TA, Lehtinen MK
bioRxiv : the preprint server for biology (2024) : . 226 008 IHC; tested species: mouse
A nociceptive amygdala-striatal pathway for chronic pain aversion.
Wojick JA, Paranjapye A, Chiu JK, Mahmood M, Oswell C, Kimmey BA, Wooldridge LM, McCall NM, Han A, Ejoh LL, Chehimi SN, et al.
bioRxiv : the preprint server for biology (2024) : . 226 008 IHC; tested species: mouse
Differences in Neuropathology between Nitroglycerin-Induced Mouse Models of Episodic and Chronic Migraine.
Park S, Jung H, Han SW, Lee SH, Sohn JH
International journal of molecular sciences (2024) 257: . 226 008 IHC; tested species: mouse
Ventral pallidum neurons projecting to the ventral tegmental area reinforce but do not invigorate reward-seeking behavior.
Palmer D, Cayton CA, Scott A, Lin I, Newell B, Paulson A, Weberg M, Richard JM
Cell reports (2024) 431: 113669. 226 008 IHC; tested species: rat
Activation of GFRAL+ neurons induces hypothermia and glucoregulatory responses associated with nausea and torpor.
Engström Ruud L, Font-Gironès F, Zajdel J, Kern L, Teixidor-Deulofeu J, Mannerås-Holm L, Carreras A, Becattini B, Björefeldt A, Hanse E, Fenselau H, et al.
Cell reports (2024) 434: 113960. 226 008 IHC; tested species: mouse
Hypothalamic astrocyte NAD+ salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity.
Park JW, Park SE, Koh W, Jang WH, Choi JH, Roh E, Kang GM, Kim SJ, Lim HS, Park CB, Jeong SY, et al.
Nature communications (2024) 151: 2102. 226 008 IHC; tested species: mouse
A microglial activation cascade across cortical regions underlies secondary mechanical hypersensitivity to amputation.
Wei HR, Tang L, Yang XL, Chen CM, Li LX, Mao Y, Wang D, Kang F, Qun S, Zhang Z, Cao P, et al.
Cell reports (2024) 432: 113804. 226 008 IHC; tested species: mouse
BLA DBS improves anxiety and fear by correcting weakened synaptic transmission from BLA to adBNST and CeL in a mouse model of foot shock.
Gao Y, Gao D, Zhang H, Zheng D, Du J, Yuan C, Mingxi Ma , Yin Y, Wang J, Zhang X, Wang Y, et al.
Cell reports (2024) 432: 113766. 226 008 IHC; tested species: mouse
Metformin Alleviates Pain States by Regulating the Balance of Spinal Synaptic Transmission.
Duan D, Wu X, Ali U, Wang D, Li X, Liu R, Ma L, Mao Y, Ma Y
Journal of integrative neuroscience (2024) 231: 6. 226 008 IHC; tested species: mouse
A mesocortical glutamatergic pathway modulates neuropathic pain independent of dopamine co-release.
Li M, Yang G
Nature communications (2024) 151: 643. 226 008 IHC; tested species: mouse
Brain stimulation-on-a-chip: a neuromodulation platform for brain slices.
Shaner S, Lu H, Lenz M, Garg S, Vlachos A, Asplund M
Lab on a chip (2023) : . 226 008 IHC; tested species: mouse
Heterogeneous complement and microglia activation mediates stress-induced synapse loss.
Soteros BM, Tillmon H, Wollet M, General J, Chin H, Lee JB, Carreno FR, Morilak DA, Kim JH, Sia GM
bioRxiv : the preprint server for biology (2023) : . 226 008 IHC; tested species: mouse
Sexually dimorphic role for insular perineuronal nets in aversion-resistant alcohol consumption.
Martins de Carvalho L, Chen H, Sutter M, Lasek AW
Frontiers in psychiatry (2023) 14: 1122423. 226 008 IHC; tested species: mouse
A neural circuit for male sexual behavior and reward.
Bayless DW, Davis CO, Yang R, Wei Y, de Andrade Carvalho VM, Knoedler JR, Yang T, Livingston O, Lomvardas A, Martins GJ, Vicente AM, et al.
Cell (2023) 18618: 3862-3881.e28. 226 008 IHC; tested species: mouse
Engram cell connectivity as a mechanism for information encoding and memory function.
Ortega-de San Luis C, Pezzoli M, Urrieta E, Ryan TJ
Current biology : CB (2023) 3324: 5368-5380.e5. 226 008 IHC; tested species: mouse
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo T, Cocas L, Pleasure S
eLife (2023) 12: . 226 008 IHC; tested species: mouse
The intersection of empathy and addiction.
Cox SS, Reichel CM
Pharmacology, biochemistry, and behavior (2023) 222: 173509. 226 008 IHC; tested species: rat
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo TT, Cocas L, Pleasure SJ
bioRxiv : the preprint server for biology (2023) : . 226 008 IHC; tested species: mouse
A Novel Single Vector Intersectional AAV Strategy for Interrogating Cellular Diversity and Brain Function.
Hughes AC, Pollard BG, Xu B, Gammons JW, Chapman P, Bikoff JB, Schwarz LA
bioRxiv : the preprint server for biology (2023) : . 226 008 IHC; tested species: mouse
NaX channel is a physiological [Na+] detector in oxytocin and vasopressin releasing magnocellular neurosecretory cells of the rat supraoptic nucleus.
Salgado-Mozo S, Thirouin ZS, Wyrosdic JC, García-Hernández U, Bourque CW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) : . 226 008 IHC; tested species: rat
Neuronal Activity Changes the Number of Neurons that are Synaptically Connected to OPCs.
Moura D, Parvathaneni A, Sahagun A, Noguchi H, Garcia J, Brennan E, Brock R, Tilton I, Halladay L, Pleasure S, Cocas L, et al.
eNeuro (2023) : . 226 008 IHC; tested species: mouse
Ultrasonocoverslip: In-vitro platform for high-throughput assay of cell type-specific neuromodulation with ultra-low-intensity ultrasound stimulation.
Lee K, Lee JM, Phan TT, Lee CJ, Park JM, Park J
Brain stimulation (2023) 165: 1533-1548. 226 008 IHC; tested species: mouse
Identification of brain areas in mice with peak neural activity across the acute and persistent phases of post-traumatic headache.
Rudolph M, Kopruszinski C, Wu C, Navratilova E, Schwedt TJ, Dodick DW, Porreca F, Anderson T
Cephalalgia : an international journal of headache (2023) 4311: 3331024231217469. 226 008 IHC; tested species: mouse
Oxytocin neurons mediate the effect of social isolation via the VTA circuits.
Musardo S, Contestabile A, Knoop M, Baud O, Bellone C
eLife (2022) 11: . 226 008 IHC; tested species: mouse
Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway.
Silva MSB, Decoster L, Trova S, Mimouni NEH, Delli V, Chachlaki K, Yu Q, Boehm U, Prevot V, Giacobini P
Proceedings of the National Academy of Sciences of the United States of America (2022) 11930: e2203503119. 226 008 IHC; tested species: mouse
Hypothalamic orexinergic neuron changes during the hibernation of the Syrian hamster.
López JM, Carballeira P, Pozo J, León-Espinosa G, Muñoz A
Frontiers in neuroanatomy (2022) 16: 993421. 226 008 IHC
Cold-sensitive ventromedial hypothalamic neurons control homeostatic thermogenesis and social interaction-associated hyperthermia.
Feng C, Wang Y, Zha X, Cao H, Huang S, Cao D, Zhang K, Xie T, Xu X, Liang Z, Zhang Z, et al.
Cell metabolism (2022) 346: 888-901.e5. 226 008 IHC; tested species: mouse
Metformin ameliorates olanzapine-induced obesity and glucose intolerance by regulating hypothalamic inflammation and microglial activation in female mice.
Suh SB, Lee N, Kim J, Kim S, Jang S, Park JK, Lee K, Choi SY, Kwon HJ, Lee CH
Frontiers in pharmacology (2022) 13: 906717. 226 008 IHC; tested species: mouse
Chloral Hydrate Alters Brain Activation Induced by Methamphetamine-Associated Cue and Prevents Relapse.
Jiang C, Xu Y, Zhong J, Wu J, He J, Xu W, Zhu Y
Frontiers in molecular neuroscience (2022) 15: 934167. 226 008 IHC; tested species: rat
Parvalbumin Neurons in Zona Incerta Regulate Itch in Mice.
Li J, Bai Y, Liang Y, Zhang Y, Zhao Q, Ge J, Li D, Zhu Y, Cai G, Tao H, Wu S, et al.
Frontiers in molecular neuroscience (2022) 15: 843754. 226 008 IHC; tested species: mouse
Crym-positive striatal astrocytes gate perseverative behaviour.
Ollivier M, Soto JS, Linker KE, Moye SL, Jami-Alahmadi Y, Jones AE, Divakaruni AS, Kawaguchi R, Wohlschlegel JA, Khakh BS
Nature (2024) : . 226 008 IHC-Fr; tested species: mouse
High-calorie diets uncouple hypothalamic oxytocin neurons from a gut-to-brain satiation pathway via κ-opioid signaling.
Gruber T, Lechner F, Murat C, Contreras RE, Sanchez-Quant E, Miok V, Makris K, Le Thuc O, González-García I, García-Clave E, Althammer F, et al.
Cell reports (2023) 4210: 113305. 226 008 iDISCO; tested species: mouse
Analyzing engram reactivation and long-range connectivity.
Refaeli R, Kreisel T, Goshen I
STAR protocols (2024) 51: 102840. 226 008 Clarity; tested species: mouse
Cat. No.: 226 008
Amount: 50 µg
Price: $425.00
A developmental brain-wide screen identifies retrosplenial cortex as a key player in the emergence of persistent memory.
Jin B, Gongwer MW, Ohanian L, Holden-Wingate L, Le B, Darmawan A, Nakayama Y, Rueda Mora SA, DeNardo LA
bioRxiv : the preprint server for biology (2024) : . 226 008 IHC; tested species: mouse
Circuit mechanisms of navigation strategy learning in mice.
Parrini M, Tricot G, Caroni P, Spolidoro M
Current biology : CB (2024) 341: 79-91.e4. 226 008 IHC; tested species: mouse
A choroid plexus apocrine secretion mechanism shapes CSF proteome and embryonic brain development.
Courtney Y, Head JP, Yimer ED, Dani N, Shipley FB, Libermann TA, Lehtinen MK
bioRxiv : the preprint server for biology (2024) : . 226 008 IHC; tested species: mouse
A nociceptive amygdala-striatal pathway for chronic pain aversion.
Wojick JA, Paranjapye A, Chiu JK, Mahmood M, Oswell C, Kimmey BA, Wooldridge LM, McCall NM, Han A, Ejoh LL, Chehimi SN, et al.
bioRxiv : the preprint server for biology (2024) : . 226 008 IHC; tested species: mouse
Differences in Neuropathology between Nitroglycerin-Induced Mouse Models of Episodic and Chronic Migraine.
Park S, Jung H, Han SW, Lee SH, Sohn JH
International journal of molecular sciences (2024) 257: . 226 008 IHC; tested species: mouse
Ventral pallidum neurons projecting to the ventral tegmental area reinforce but do not invigorate reward-seeking behavior.
Palmer D, Cayton CA, Scott A, Lin I, Newell B, Paulson A, Weberg M, Richard JM
Cell reports (2024) 431: 113669. 226 008 IHC; tested species: rat
Activation of GFRAL+ neurons induces hypothermia and glucoregulatory responses associated with nausea and torpor.
Engström Ruud L, Font-Gironès F, Zajdel J, Kern L, Teixidor-Deulofeu J, Mannerås-Holm L, Carreras A, Becattini B, Björefeldt A, Hanse E, Fenselau H, et al.
Cell reports (2024) 434: 113960. 226 008 IHC; tested species: mouse
Hypothalamic astrocyte NAD+ salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity.
Park JW, Park SE, Koh W, Jang WH, Choi JH, Roh E, Kang GM, Kim SJ, Lim HS, Park CB, Jeong SY, et al.
Nature communications (2024) 151: 2102. 226 008 IHC; tested species: mouse
A microglial activation cascade across cortical regions underlies secondary mechanical hypersensitivity to amputation.
Wei HR, Tang L, Yang XL, Chen CM, Li LX, Mao Y, Wang D, Kang F, Qun S, Zhang Z, Cao P, et al.
Cell reports (2024) 432: 113804. 226 008 IHC; tested species: mouse
BLA DBS improves anxiety and fear by correcting weakened synaptic transmission from BLA to adBNST and CeL in a mouse model of foot shock.
Gao Y, Gao D, Zhang H, Zheng D, Du J, Yuan C, Mingxi Ma , Yin Y, Wang J, Zhang X, Wang Y, et al.
Cell reports (2024) 432: 113766. 226 008 IHC; tested species: mouse
Metformin Alleviates Pain States by Regulating the Balance of Spinal Synaptic Transmission.
Duan D, Wu X, Ali U, Wang D, Li X, Liu R, Ma L, Mao Y, Ma Y
Journal of integrative neuroscience (2024) 231: 6. 226 008 IHC; tested species: mouse
A mesocortical glutamatergic pathway modulates neuropathic pain independent of dopamine co-release.
Li M, Yang G
Nature communications (2024) 151: 643. 226 008 IHC; tested species: mouse
Brain stimulation-on-a-chip: a neuromodulation platform for brain slices.
Shaner S, Lu H, Lenz M, Garg S, Vlachos A, Asplund M
Lab on a chip (2023) : . 226 008 IHC; tested species: mouse
Heterogeneous complement and microglia activation mediates stress-induced synapse loss.
Soteros BM, Tillmon H, Wollet M, General J, Chin H, Lee JB, Carreno FR, Morilak DA, Kim JH, Sia GM
bioRxiv : the preprint server for biology (2023) : . 226 008 IHC; tested species: mouse
Sexually dimorphic role for insular perineuronal nets in aversion-resistant alcohol consumption.
Martins de Carvalho L, Chen H, Sutter M, Lasek AW
Frontiers in psychiatry (2023) 14: 1122423. 226 008 IHC; tested species: mouse
A neural circuit for male sexual behavior and reward.
Bayless DW, Davis CO, Yang R, Wei Y, de Andrade Carvalho VM, Knoedler JR, Yang T, Livingston O, Lomvardas A, Martins GJ, Vicente AM, et al.
Cell (2023) 18618: 3862-3881.e28. 226 008 IHC; tested species: mouse
Engram cell connectivity as a mechanism for information encoding and memory function.
Ortega-de San Luis C, Pezzoli M, Urrieta E, Ryan TJ
Current biology : CB (2023) 3324: 5368-5380.e5. 226 008 IHC; tested species: mouse
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo T, Cocas L, Pleasure S
eLife (2023) 12: . 226 008 IHC; tested species: mouse
The intersection of empathy and addiction.
Cox SS, Reichel CM
Pharmacology, biochemistry, and behavior (2023) 222: 173509. 226 008 IHC; tested species: rat
Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging.
Noguchi H, Arela JC, Ngo TT, Cocas L, Pleasure SJ
bioRxiv : the preprint server for biology (2023) : . 226 008 IHC; tested species: mouse
A Novel Single Vector Intersectional AAV Strategy for Interrogating Cellular Diversity and Brain Function.
Hughes AC, Pollard BG, Xu B, Gammons JW, Chapman P, Bikoff JB, Schwarz LA
bioRxiv : the preprint server for biology (2023) : . 226 008 IHC; tested species: mouse
NaX channel is a physiological [Na+] detector in oxytocin and vasopressin releasing magnocellular neurosecretory cells of the rat supraoptic nucleus.
Salgado-Mozo S, Thirouin ZS, Wyrosdic JC, García-Hernández U, Bourque CW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) : . 226 008 IHC; tested species: rat
Neuronal Activity Changes the Number of Neurons that are Synaptically Connected to OPCs.
Moura D, Parvathaneni A, Sahagun A, Noguchi H, Garcia J, Brennan E, Brock R, Tilton I, Halladay L, Pleasure S, Cocas L, et al.
eNeuro (2023) : . 226 008 IHC; tested species: mouse
Ultrasonocoverslip: In-vitro platform for high-throughput assay of cell type-specific neuromodulation with ultra-low-intensity ultrasound stimulation.
Lee K, Lee JM, Phan TT, Lee CJ, Park JM, Park J
Brain stimulation (2023) 165: 1533-1548. 226 008 IHC; tested species: mouse
Identification of brain areas in mice with peak neural activity across the acute and persistent phases of post-traumatic headache.
Rudolph M, Kopruszinski C, Wu C, Navratilova E, Schwedt TJ, Dodick DW, Porreca F, Anderson T
Cephalalgia : an international journal of headache (2023) 4311: 3331024231217469. 226 008 IHC; tested species: mouse
Oxytocin neurons mediate the effect of social isolation via the VTA circuits.
Musardo S, Contestabile A, Knoop M, Baud O, Bellone C
eLife (2022) 11: . 226 008 IHC; tested species: mouse
Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway.
Silva MSB, Decoster L, Trova S, Mimouni NEH, Delli V, Chachlaki K, Yu Q, Boehm U, Prevot V, Giacobini P
Proceedings of the National Academy of Sciences of the United States of America (2022) 11930: e2203503119. 226 008 IHC; tested species: mouse
Hypothalamic orexinergic neuron changes during the hibernation of the Syrian hamster.
López JM, Carballeira P, Pozo J, León-Espinosa G, Muñoz A
Frontiers in neuroanatomy (2022) 16: 993421. 226 008 IHC
Cold-sensitive ventromedial hypothalamic neurons control homeostatic thermogenesis and social interaction-associated hyperthermia.
Feng C, Wang Y, Zha X, Cao H, Huang S, Cao D, Zhang K, Xie T, Xu X, Liang Z, Zhang Z, et al.
Cell metabolism (2022) 346: 888-901.e5. 226 008 IHC; tested species: mouse
Metformin ameliorates olanzapine-induced obesity and glucose intolerance by regulating hypothalamic inflammation and microglial activation in female mice.
Suh SB, Lee N, Kim J, Kim S, Jang S, Park JK, Lee K, Choi SY, Kwon HJ, Lee CH
Frontiers in pharmacology (2022) 13: 906717. 226 008 IHC; tested species: mouse
Chloral Hydrate Alters Brain Activation Induced by Methamphetamine-Associated Cue and Prevents Relapse.
Jiang C, Xu Y, Zhong J, Wu J, He J, Xu W, Zhu Y
Frontiers in molecular neuroscience (2022) 15: 934167. 226 008 IHC; tested species: rat
Parvalbumin Neurons in Zona Incerta Regulate Itch in Mice.
Li J, Bai Y, Liang Y, Zhang Y, Zhao Q, Ge J, Li D, Zhu Y, Cai G, Tao H, Wu S, et al.
Frontiers in molecular neuroscience (2022) 15: 843754. 226 008 IHC; tested species: mouse
Crym-positive striatal astrocytes gate perseverative behaviour.
Ollivier M, Soto JS, Linker KE, Moye SL, Jami-Alahmadi Y, Jones AE, Divakaruni AS, Kawaguchi R, Wohlschlegel JA, Khakh BS
Nature (2024) : . 226 008 IHC-Fr; tested species: mouse
High-calorie diets uncouple hypothalamic oxytocin neurons from a gut-to-brain satiation pathway via κ-opioid signaling.
Gruber T, Lechner F, Murat C, Contreras RE, Sanchez-Quant E, Miok V, Makris K, Le Thuc O, González-García I, García-Clave E, Althammer F, et al.
Cell reports (2023) 4210: 113305. 226 008 iDISCO; tested species: mouse
Analyzing engram reactivation and long-range connectivity.
Refaeli R, Kreisel T, Goshen I
STAR protocols (2024) 51: 102840. 226 008 Clarity; tested species: mouse
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).