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c-Fos - 226 008

A member of the transcription factor complex AP1
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 226 008
Amount: 50 µg
Price: 415.00 €
Cat. No. 226 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery  
Clone Rb108B5
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 2 to 17 from rat c-Fos (UniProt Id: P12841)
Reactivity Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
Specificity Specific for c-Fos
Matching control protein/peptide 226-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: Signal quality is strongly enhanced when antibody is incubated at RT. For best results, tissue sections should be stored at -20°C in cryoprotectant solution. Prolonged storage at 4°C leads to a substantial loss of signal.

Data sheet 226_008.pdf
Cat. No.: 226 008
Quantity: 50 µg
Price: 415.00 €
Background

The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).