|Cat. No. 226 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 1000 gallery
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 2 to 17 from rat c-Fos (UniProt Id: P12841)|
Reacts with: mouse (P01101), rat (P12841), human (P01100).
Other species not tested yet.
|Specificity||Specific for c-Fos|
|Matching control protein/peptide||226-0P|
This antibody is a chimeric antibody based on the monoclonal rat antibody clone 108B5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
The Fos gene family consists of 4 members: c-Fos, FosB, FosL1, and FosL2, also called Fos related antigen 1 and 2 (FRA1 and 2). These leucine zipper proteins can dimerize with proteins of the JUN family leading to the formation of the transcription factor complex AP1 (1).
The expression of Fos proteins is rapidly and transiently induced by different extracellular stimuli such as growth factors, cytokines, neurotransmitters, polypeptide hormones, stress (2).
In addition Fos proteins can be phosphorylated by ERK kinases modulating transcriptional activity, protein stability and localization (3). c-Fos is the homologue to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene (4).