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GFP - 132 111

A universal tool in cell biology
Monoclonal mouse purified IgG
Cat. No.: 132 111
Amount: 100 µg
Price: 415.00 €
Cat. No. 132 111 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: not recommended (see remarks)
ICC: yes (methanol fixated material only) (see remarks)
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone 101G4
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 238 from GFP (UniProt Id: P42212)
Epitop Epitop: AA 183 to 191 from GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks

IP: Cat. no. 132 011 or 132 002 is recommended for IP.

ICC: Cat. no. 132 011 or 132 002 is recommended for ICC.

Data sheet 132_111.pdf

References for GFP - 132 111

Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin.
Koestler SA, Steffen A, Nemethova M, Winterhoff M, Luo N, Holleboom JM, Krupp J, Jacob S, Vinzenz M, Schur F, Schlüter K, et al.
Molecular biology of the cell (2013) 2418: 2861-75. 132 111 WB
Microtubules as platforms for assaying actin polymerization in vivo.
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, et al.
PloS one (2011) 65: e19931. 132 111 WB
Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte.
Kaddai V, Gonzalez T, Keslair F, Grémeaux T, Bonnafous S, Gugenheim J, Tran A, Gual P, Le Marchand-Brustel Y, Cormont M
PloS one (2009) 44: e5257. 132 111 WB
Cat. No.: 132 111
Quantity: 100 µg
Price: 415.00 €
Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin.
Koestler SA, Steffen A, Nemethova M, Winterhoff M, Luo N, Holleboom JM, Krupp J, Jacob S, Vinzenz M, Schur F, Schlüter K, et al.
Molecular biology of the cell (2013) 2418: 2861-75. 132 111 WB
Microtubules as platforms for assaying actin polymerization in vivo.
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, et al.
PloS one (2011) 65: e19931. 132 111 WB
Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte.
Kaddai V, Gonzalez T, Keslair F, Grémeaux T, Bonnafous S, Gugenheim J, Tran A, Gual P, Le Marchand-Brustel Y, Cormont M
PloS one (2009) 44: e5257. 132 111 WB
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.