Synaptic Systems

Cat. No.: 132 111

Amount: 100 µg

Price: $415.00

Cat. No. 132 111	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: not recommended (see remarks) ICC: yes (methanol fixated material only) (see remarks) IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	101G4
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Epitop	AA 183 to 191 from jellyfish GFP (UniProt Id: P42212)
Specificity	Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks	IP: Cat. no. 132 011 or 132 002 is recommended for IP. ICC: Cat. no. 132 011 or 132 002 is recommended for ICC.
Data sheet	132_111.pdf

References for GFP - 132 111

FOXR1 regulates stress response pathways and is necessary for proper brain development.
Mota A, Waxman HK, Hong R, Lagani GD, Niu SY, Bertherat FL, Wolfe L, Malicdan CM, Markello TC, Adams DR, Gahl WA, et al.
PLoS genetics (2021) 1711: e1009854. 132 111 WB; tested species: human

Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin.
Koestler SA, Steffen A, Nemethova M, Winterhoff M, Luo N, Holleboom JM, Krupp J, Jacob S, Vinzenz M, Schur F, Schlüter K, et al.
Molecular biology of the cell (2013) 2418: 2861-75. 132 111 WB

Microtubules as platforms for assaying actin polymerization in vivo.
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, et al.
PloS one (2011) 65: e19931. 132 111 WB

Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte.
Kaddai V, Gonzalez T, Keslair F, Grémeaux T, Bonnafous S, Gugenheim J, Tran A, Gual P, Le Marchand-Brustel Y, Cormont M
PloS one (2009) 44: e5257. 132 111 WB

Cat. No.: 132 111

Amount: 100 µg

Price: $415.00

Background

Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.

1 ) van Roessel P et al., Nat. Cell Biol. (2002)
2 ) Stephens DJ et al., J. Cell. Sci. (2001)
3 ) Chamberlain C et al., Traffic (2000)

Protocols

Other products for this target

GFP antibody - 132 111

References for GFP - 132 111