Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 132 003

GFP antibody - 132 003

GFP is a widely used green fluorescent protein tag
Rabbit polyclonal purified antibody
Cat. No.: 132 003
Amount: 50 µg
Price: $455.00
Cat. No. 132 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 20000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet 132_003.pdf

References for GFP - 132 003

The catalytic function of the gephyrin-binding protein IQSEC3 regulates neurotransmitter-specific matching of pre- and postsynaptic structures in primary hippocampal cultures.
Früh S, Tyagarajan SK, Campbell B, Bosshard G, Fritschy JM
Journal of neurochemistry (2018) : . 132 003 WB; tested species: rat
Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down.
Chowdhury D, Turner M, Patriarchi T, Hergarden AC, Anderson D, Zhang Y, Sun J, Chen CY, Ames JB, Hell JW
The EMBO journal (2018) 371: 122-138. 132 003 IP
The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 132 003 IP
Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.
Chowdhury D, Marco S, Brooks IM, Zandueta A, Rao Y, Haucke V, Wesseling JF, Tavalin SJ, Pérez-Otaño I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 339: 4151-64. 132 003 IP, ICC
Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity.
Rotmann A, Strand D, Martiné U, Closs EI
The Journal of biological chemistry (2004) 27952: 54185-92. 132 003 IP
DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease.
Schmidt ME, Caron NS, Aly AE, Lemarié FL, Dal Cengio L, Ko Y, Lazic N, Anderson L, Nguyen B, Raymond LA, Hayden MR, et al.
Frontiers in cellular neuroscience (2020) 14: 590569. 132 003 ICC; tested species: mouse
Bällchen is required for self-renewal of germline stem cells in Drosophila melanogaster.
Herzig B, Yakulov TA, Klinge K, Günesdogan U, Jäckle H, Herzig A
Biology open (2014) 36: 510-21. 132 003 ICC
Titration of Syntaxin1 in mammalian synapses reveals multiple roles in vesicle docking, priming, and release probability.
Arancillo M, Min SW, Gerber S, Münster-Wandowski A, Wu YJ, Herman M, Trimbuch T, Rah JC, Ahnert-Hilger G, Riedel D, Südhof TC, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16698-714. 132 003 ICC
Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.
Chowdhury D, Marco S, Brooks IM, Zandueta A, Rao Y, Haucke V, Wesseling JF, Tavalin SJ, Pérez-Otaño I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 339: 4151-64. 132 003 IP, ICC
Rescue of Normal Excitability in LGI1-Deficient Epileptic Neurons.
Extrémet J, Ramirez-Franco J, Fronzaroli-Molinieres L, Boumedine-Guignon N, Ankri N, El Far O, Garrido JJ, Debanne D, Russier M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4350: 8596-8606. 132 003 IHC; tested species: mouse
NMDA receptor-targeted enrichment of CaMKIIα improves fear memory.
Chifor A, Choi J, Park J
iScience (2022) 258: 104864. 132 003 IHC; tested species: mouse
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 132 003 IHC
Drosophila gene tao-1 encodes proteins with and without a Ste20 kinase domain that affect cytoskeletal architecture and cell migration differently.
Pflanz R, Voigt A, Yakulov T, Jäckle H
Open biology (2015) 51: 140161. 132 003 IHC
Morphological, biophysical and synaptic properties of glutamatergic neurons of the mouse spinal dorsal horn.
Punnakkal P, von Schoultz C, Haenraets K, Wildner H, Zeilhofer HU
The Journal of physiology (2014) 5924: 759-76. 132 003 IHC
Oligodendrocyte dynamics in the healthy adult CNS: evidence for myelin remodeling.
Young KM, Psachoulia K, Tripathi RB, Dunn SJ, Cossell L, Attwell D, Tohyama K, Richardson WD
Neuron (2013) 775: 873-85. 132 003 IHC; tested species: mouse
Selective regulation of NR2B by protein phosphatase-1 for the control of the NMDA receptor in neuroprotection.
Farinelli M, Heitz FD, Grewe BF, Tyagarajan SK, Helmchen F, Mansuy IM
PloS one (2012) 73: e34047. 132 003 IHC
The short-time structural plasticity of dendritic spines is altered in a model of Rett syndrome.
Landi S, Putignano E, Boggio EM, Giustetto M, Pizzorusso T, Ratto GM
Scientific reports (2011) 1: 45. 132 003 IHC; tested species: mouse
Cat. No.: 132 003
Amount: 50 µg
Price: $455.00
The catalytic function of the gephyrin-binding protein IQSEC3 regulates neurotransmitter-specific matching of pre- and postsynaptic structures in primary hippocampal cultures.
Früh S, Tyagarajan SK, Campbell B, Bosshard G, Fritschy JM
Journal of neurochemistry (2018) : . 132 003 WB; tested species: rat
Ca2+/calmodulin binding to PSD-95 mediates homeostatic synaptic scaling down.
Chowdhury D, Turner M, Patriarchi T, Hergarden AC, Anderson D, Zhang Y, Sun J, Chen CY, Ames JB, Hell JW
The EMBO journal (2018) 371: 122-138. 132 003 IP
The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 132 003 IP
Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.
Chowdhury D, Marco S, Brooks IM, Zandueta A, Rao Y, Haucke V, Wesseling JF, Tavalin SJ, Pérez-Otaño I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 339: 4151-64. 132 003 IP, ICC
Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity.
Rotmann A, Strand D, Martiné U, Closs EI
The Journal of biological chemistry (2004) 27952: 54185-92. 132 003 IP
DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease.
Schmidt ME, Caron NS, Aly AE, Lemarié FL, Dal Cengio L, Ko Y, Lazic N, Anderson L, Nguyen B, Raymond LA, Hayden MR, et al.
Frontiers in cellular neuroscience (2020) 14: 590569. 132 003 ICC; tested species: mouse
Bällchen is required for self-renewal of germline stem cells in Drosophila melanogaster.
Herzig B, Yakulov TA, Klinge K, Günesdogan U, Jäckle H, Herzig A
Biology open (2014) 36: 510-21. 132 003 ICC
Titration of Syntaxin1 in mammalian synapses reveals multiple roles in vesicle docking, priming, and release probability.
Arancillo M, Min SW, Gerber S, Münster-Wandowski A, Wu YJ, Herman M, Trimbuch T, Rah JC, Ahnert-Hilger G, Riedel D, Südhof TC, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16698-714. 132 003 ICC
Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.
Chowdhury D, Marco S, Brooks IM, Zandueta A, Rao Y, Haucke V, Wesseling JF, Tavalin SJ, Pérez-Otaño I
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 339: 4151-64. 132 003 IP, ICC
Rescue of Normal Excitability in LGI1-Deficient Epileptic Neurons.
Extrémet J, Ramirez-Franco J, Fronzaroli-Molinieres L, Boumedine-Guignon N, Ankri N, El Far O, Garrido JJ, Debanne D, Russier M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4350: 8596-8606. 132 003 IHC; tested species: mouse
NMDA receptor-targeted enrichment of CaMKIIα improves fear memory.
Chifor A, Choi J, Park J
iScience (2022) 258: 104864. 132 003 IHC; tested species: mouse
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.
Hassouna I, Ott C, Wüstefeld L, Offen N, Neher RA, Mitkovski M, Winkler D, Sperling S, Fries L, Goebbels S, Vreja IC, et al.
Molecular psychiatry (2016) 2112: 1752-1767. 132 003 IHC
Drosophila gene tao-1 encodes proteins with and without a Ste20 kinase domain that affect cytoskeletal architecture and cell migration differently.
Pflanz R, Voigt A, Yakulov T, Jäckle H
Open biology (2015) 51: 140161. 132 003 IHC
Morphological, biophysical and synaptic properties of glutamatergic neurons of the mouse spinal dorsal horn.
Punnakkal P, von Schoultz C, Haenraets K, Wildner H, Zeilhofer HU
The Journal of physiology (2014) 5924: 759-76. 132 003 IHC
Oligodendrocyte dynamics in the healthy adult CNS: evidence for myelin remodeling.
Young KM, Psachoulia K, Tripathi RB, Dunn SJ, Cossell L, Attwell D, Tohyama K, Richardson WD
Neuron (2013) 775: 873-85. 132 003 IHC; tested species: mouse
Selective regulation of NR2B by protein phosphatase-1 for the control of the NMDA receptor in neuroprotection.
Farinelli M, Heitz FD, Grewe BF, Tyagarajan SK, Helmchen F, Mansuy IM
PloS one (2012) 73: e34047. 132 003 IHC
The short-time structural plasticity of dendritic spines is altered in a model of Rett syndrome.
Landi S, Putignano E, Boggio EM, Giustetto M, Pizzorusso T, Ratto GM
Scientific reports (2011) 1: 45. 132 003 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.
Other products for this target