Tailor-made Antibodies
and Tools for Life Science

GFP antibody - 132 004

GFP is a widely used green fluorescent protein tag
Guinea pig polyclonal antiserum
Cat. No.: 132 004
Amount: 100 µl
Price: $370.00
Cat. No. 132 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
EM: yes
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants. This antibody still recognizes its antigen after heat mediated antigen retrieval. It does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks

IHC: The unpurified serum tends to stain nuclear structures in WT tissue, that lacks GFP expression. The affinity purified version 132 005 recommended.

Data sheet 132_004.pdf

References for GFP - 132 004

S-SCAM is essential for synapse formation.
Wittenmayer N, Petkova-Tuffy A, Borgmeyer M, Lee C, Becker J, Böning A, Kügler S, Rhee J, Viotti JS, Dresbach T
Frontiers in cellular neuroscience (2023) 17: 1182493. 132 004 WB; tested species: rat
Cat. No.: 132 004
Amount: 100 µl
Price: $370.00