Synaptic Systems

Cat. No.: 132 004

Amount: 100 µl

Price: $365.00

Cat. No. 132 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity	Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants. This antibody still recognizes its antigen after heat mediated antigen retrieval. It does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks	IHC: The unpurified serum tends to stain nuclear structures in WT tissue, that lacks GFP expression. The affinity purified version 132 005 recommended.
Data sheet	132_004.pdf

References for GFP - 132 004

S-SCAM is essential for synapse formation.
Wittenmayer N, Petkova-Tuffy A, Borgmeyer M, Lee C, Becker J, Böning A, Kügler S, Rhee J, Viotti JS, Dresbach T
Frontiers in cellular neuroscience (2023) 17: 1182493. 132 004 WB; tested species: rat

Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 132 004 ICC; tested species: mouse

Activity in the dorsal hippocampus-mPFC circuit modulates stress-coping strategies during inescapable stress.
Yoon SH, Song WS, Chung G, Kim SJ, Kim MH
Experimental & molecular medicine (2024) : . 132 004 IHC; tested species: mouse

Immune response and pathogen invasion at the choroid plexus in the onset of cerebral toxoplasmosis.
Figueiredo CA, Steffen J, Morton L, Arumugam S, Liesenfeld O, Deli MA, Kröger A, Schüler T, Dunay IR
Journal of neuroinflammation (2022) 191: 17. 132 004 IHC; tested species: mouse

Impaired synaptic transmission in dorsal dentate gyrus increases impulsive alcohol seeking.
Nalberczak-Skóra M, Beroun A, Skonieczna E, Cały A, Ziółkowska M, Pagano R, Taheri P, Kalita K, Salamian A, Radwanska K
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) : . 132 004 IHC; tested species: mouse

The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.
Lambert E, Saha O, Soares Landeira B, Melo de Farias AR, Hermant X, Carrier A, Pelletier A, Gadaut J, Davoine L, Dupont C, Amouyel P, et al.
Acta neuropathologica communications (2022) 101: 4. 132 004 IHC; tested species: drosophila

Optogenetic stimulation of striatal patches modifies habit formation and inhibits dopamine release.
Nadel JA, Pawelko SS, Scott JR, McLaughlin R, Fox M, Ghanem M, van der Merwe R, Hollon NG, Ramsson ES, Howard CD
Scientific reports (2021) 111: 19847. 132 004 IHC; tested species: mouse

Novel 3D analysis using optical tissue clearing documents the evolution of murine rapidly progressive glomerulonephritis.
Puelles VG, Fleck D, Ortz L, Papadouri S, Strieder T, Böhner AMC, van der Wolde JW, Vogt M, Saritas T, Kuppe C, Fuss A, et al.
Kidney international (2019) 962: 505-516. 132 004 IHC; tested species: mouse

An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 132 004 IHC; tested species: mouse

Postsynaptic gephyrin clustering controls the development of adult-born granule cells in the olfactory bulb.
Deprez F, Pallotto M, Vogt F, Grabiec M, Virtanen MA, Tyagarajan SK, Panzanelli P, Fritschy JM
The Journal of comparative neurology (2015) 52313: 1998-2016. 132 004 EM

Cat. No.: 132 004

Amount: 100 µl

Price: $365.00

ICC

IHC

Background

Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.

1 ) van Roessel P et al., Nat. Cell Biol. (2002)
2 ) Stephens DJ et al., J. Cell. Sci. (2001)
3 ) Chamberlain C et al., Traffic (2000)

Protocols

ICC

IHC

Other products for this target

GFP antibody - 132 004

References for GFP - 132 004