Synaptic Systems

Cat. No.: 132 005

Amount: 50 µg

Price: $470.00

Cat. No. 132 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity	Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet	Datasheet 132_005

References for GFP - 132 005

Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 132 005 ICC; tested species: mouse

JNK signalling regulates antioxidant responses in neurons.
Ugbode C, Garnham N, Fort-Aznar L, Evans GJO, Chawla S, Sweeney ST
Redox biology (2020) 37: 101712. 132 005 ICC; tested species: rat

Neuroprotective activity of ursodeoxycholic acid in CHMP2BIntron5 models of frontotemporal dementia.
West RJH, Ugbode C, Fort-Aznar L, Sweeney ST
Neurobiology of disease (2020) 144: 105047. 132 005 ICC; tested species: rat

TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 132 005 ICC, IHC; tested species: rat

Epitope specificity of anti-synapsin autoantibodies: Differential targeting of synapsin I domains.
Mertens R, Melchert S, Gitler D, Schou MB, Saether SG, Vaaler A, Piepgras J, Kochova E, Benfenati F, Ahnert-Hilger G, Ruprecht K, et al.
PloS one (2018) 1312: e0208636. 132 005 ICC; tested species: human

Transcription factor 21 deletion from podocyte precursors as a model for congenital nephrotic syndrome.
Dalal V, Zhou Y, Deb DK, Thomson BR, Nelson JW, Ledogar G, Misener S, Maekawa H, Chang A, Chung E, Park JS, et al.
American journal of physiology. Renal physiology (2026) 3306: F746-F760. 132 005 IHC; tested species: mouse

Dorsal hippocampal CA3 to CA1 circuit maintains contextual cocaine memories in a rat model of drug relapse.
Qi S, Soto DA, Pruitt AY, Ritchie JL, Reeves DA, Fuchs RA
Neuropharmacology (2026) 298: 111064. 132 005 IHC; tested species: rat

Differentiated Presynaptic Input to OLMɑ2 Cells Along the Hippocampal Dorsoventral Axis: Implications for Hippocampal Microcircuit Function.
Thulin A, Henriksson K, Nogueira I, Kullander K
Hippocampus (2025) 355: e70026. 132 005 IHC; tested species: mouse

A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level.
Merolla A, Michetti C, Moschetta M, Vacca F, Ciano L, Emionite L, Astigiano S, Romei A, Horenkamp S, Berglund K, Gross RE, et al.
Nature communications (2024) 151: 5609. 132 005 IHC; tested species: mouse

A Selective Projection from the Subthalamic Nucleus to Parvalbumin-Expressing Interneurons of the Striatum.
Kondabolu K, Doig NM, Ayeko O, Khan B, Torres A, Calvigioni D, Meletis K, Koós T, Magill PJ
eNeuro (2023) 107: . 132 005 IHC; tested species: mouse

Pharmacological signature and target-specificity of inhibitory circuits formed by Martinotti cells in the mouse barrel cortex.
Donato C, Victorino DB, Cabezas C, Aguirre A, Lourenço J, Potier MC, de San Martin JZ, Bacci A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 132 005 IHC; tested species: mouse

The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells.
Fadiga J, Nystul TG
eLife (2019) 8: . 132 005 IHC; tested species: drosophila

Neuronal activity regulates neurotransmitter switching in the adult brain following light-induced stress.
Meng D, Li HQ, Deisseroth K, Leutgeb S, Spitzer NC
Proceedings of the National Academy of Sciences of the United States of America (2018) : . 132 005 IHC; tested species: rat

Phosphorylated Groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche.
Johnston MJ, Bar-Cohen S, Paroush Z, Nystul TG
Development (Cambridge, England) (2016) 14324: 4631-4642. 132 005 IHC

A positive feedback loop between germ cells and gonads induces and maintains sexual reproduction in a cnidarian.
Curantz C, Doody C, Horkan HR, Krasovec G, Weavers PK, DuBuc TQ, Frank U
Science advances (2025) 112: eadq8220. 132 005

Cat. No.: 132 005

Amount: 50 µg

Price: $470.00

Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 132 005 ICC; tested species: mouse

JNK signalling regulates antioxidant responses in neurons.
Ugbode C, Garnham N, Fort-Aznar L, Evans GJO, Chawla S, Sweeney ST
Redox biology (2020) 37: 101712. 132 005 ICC; tested species: rat

The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells.
Fadiga J, Nystul TG
eLife (2019) 8: . 132 005 IHC; tested species: drosophila

WB
(1 img)

ICC
(1 img)

IHC
(4 img)

Background

Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.

1 ) van Roessel P et al., Nat. Cell Biol. (2002)
2 ) Stephens DJ et al., J. Cell. Sci. (2001)
3 ) Chamberlain C et al., Traffic (2000)

Protocols

ICC

Fixed Cells

IHC

Other products for this target

GFP antibody - 132 005

References for GFP - 132 005