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GFP antibody - 132 006

GFP is a widely used green fluorescent protein tag
Chicken polyclonal purified antibody
Cat. No.: 132 006
Amount: 50 µg
Price: $380.00
Cat. No. 132 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet 132_006.pdf

References for GFP - 132 006

Neuron-derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB-mediated signalling and preserve neuronal complexity.
Solana-Balaguer J, Campoy-Campos G, Martín-Flores N, Pérez-Sisqués L, Sitjà-Roqueta L, Kucukerden M, Gámez-Valero A, Coll-Manzano A, Martí E, Pérez-Navarro E, Alberch J, et al.
Journal of extracellular vesicles (2023) 129: e12355. 132 006 ICC; tested species: rat
S-SCAM is essential for synapse formation.
Wittenmayer N, Petkova-Tuffy A, Borgmeyer M, Lee C, Becker J, Böning A, Kügler S, Rhee J, Viotti JS, Dresbach T
Frontiers in cellular neuroscience (2023) 17: 1182493. 132 006 ICC; tested species: rat
5-HT4R agonism reduces L-DOPA-induced dyskinesia via striatopallidal neurons in unilaterally 6-OHDA lesioned mice.
Ballardin D, Makrini-Maleville L, Seper A, Valjent E, Rebholz H
Neurobiology of disease (2024) 198: 106559. 132 006 IHC; tested species: mouse
Synaptic communication within the microcircuits of pyramidal neurons and basket cells in the mouse prefrontal cortex.
Fekete Z, Weisz F, Karlócai MR, Veres JM, Andrási T, Hájos N
The Journal of physiology (2024) : . 132 006 IHC; tested species: mouse
CaMKIIα Promoter-Controlled Circuit Manipulations Target Both Pyramidal Cells and Inhibitory Interneurons in Cortical Networks.
Veres JM, Andrasi T, Nagy-Pal P, Hajos N
eNeuro (2023) 104: . 132 006 IHC; tested species: mouse
RTP801/REDD1 Is Involved in Neuroinflammation and Modulates Cognitive Dysfunction in Huntington's Disease.
Pérez-Sisqués L, Solana-Balaguer J, Campoy-Campos G, Martín-Flores N, Sancho-Balsells A, Vives-Isern M, Soler-Palazón F, Garcia-Forn M, Masana M, Alberch J, Pérez-Navarro E, et al.
Biomolecules (2021) 121: . 132 006 IHC; tested species: mouse
Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca2+ transients and neurovascular coupling.
Beiersdorfer A, Scheller A, Kirchhoff F, Lohr C
Glia (2019) : . 132 006 IHC; tested species: mouse
Cat. No.: 132 006
Amount: 50 µg
Price: $380.00
Neuron-derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB-mediated signalling and preserve neuronal complexity.
Solana-Balaguer J, Campoy-Campos G, Martín-Flores N, Pérez-Sisqués L, Sitjà-Roqueta L, Kucukerden M, Gámez-Valero A, Coll-Manzano A, Martí E, Pérez-Navarro E, Alberch J, et al.
Journal of extracellular vesicles (2023) 129: e12355. 132 006 ICC; tested species: rat
S-SCAM is essential for synapse formation.
Wittenmayer N, Petkova-Tuffy A, Borgmeyer M, Lee C, Becker J, Böning A, Kügler S, Rhee J, Viotti JS, Dresbach T
Frontiers in cellular neuroscience (2023) 17: 1182493. 132 006 ICC; tested species: rat
5-HT4R agonism reduces L-DOPA-induced dyskinesia via striatopallidal neurons in unilaterally 6-OHDA lesioned mice.
Ballardin D, Makrini-Maleville L, Seper A, Valjent E, Rebholz H
Neurobiology of disease (2024) 198: 106559. 132 006 IHC; tested species: mouse
Synaptic communication within the microcircuits of pyramidal neurons and basket cells in the mouse prefrontal cortex.
Fekete Z, Weisz F, Karlócai MR, Veres JM, Andrási T, Hájos N
The Journal of physiology (2024) : . 132 006 IHC; tested species: mouse
CaMKIIα Promoter-Controlled Circuit Manipulations Target Both Pyramidal Cells and Inhibitory Interneurons in Cortical Networks.
Veres JM, Andrasi T, Nagy-Pal P, Hajos N
eNeuro (2023) 104: . 132 006 IHC; tested species: mouse
RTP801/REDD1 Is Involved in Neuroinflammation and Modulates Cognitive Dysfunction in Huntington's Disease.
Pérez-Sisqués L, Solana-Balaguer J, Campoy-Campos G, Martín-Flores N, Sancho-Balsells A, Vives-Isern M, Soler-Palazón F, Garcia-Forn M, Masana M, Alberch J, Pérez-Navarro E, et al.
Biomolecules (2021) 121: . 132 006 IHC; tested species: mouse
Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca2+ transients and neurovascular coupling.
Beiersdorfer A, Scheller A, Kirchhoff F, Lohr C
Glia (2019) : . 132 006 IHC; tested species: mouse
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.