Tailor-made Antibodies
and Tools for Life Science

GFP antibody - 132 011

GFP is a widely used green fluorescent protein tag
Mouse monoclonal purified IgG
Cat. No.: 132 011
Amount: 100 µg
Price: $420.00
Cat. No. 132 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: yes
ICC: 1 : 100 gallery  
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
Clone 270F3
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Epitop AA 183 to 188 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks

Cat. no. 132 111 or 132 002 is recommended for WB.
IHC: Unspecific labeling of astrocytes may occur in the absence of GFP in wildtype tissue.

Data sheet 132_011.pdf

References for GFP - 132 011

SNAP-25 phosphorylation at Ser187 is not involved in Ca2+ or phorbolester-dependent potentiation of synaptic release.
Ruiter M, Houy S, Engholm-Keller K, Graham ME, Sørensen JB
Molecular and cellular neurosciences (2019) : 103452. 132 011 WB; tested species: mouse
Cat. No.: 132 011
Amount: 100 µg
Price: $420.00