Synaptic Systems

Cat. No.: 132 011

Amount: 100 µg

Price: $415.00

Cat. No. 132 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: 1 : 100 gallery IHC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	270F3
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Epitop	AA 183 to 188 from jellyfish GFP (UniProt Id: P42212)
Specificity	Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks	Cat. no. 132 111 or 132 002 is recommended for WB. IHC: Unspecific labeling of astrocytes may occur in the absence of GFP in wildtype tissue.
Data sheet	132_011.pdf

References for GFP - 132 011

SNAP-25 phosphorylation at Ser187 is not involved in Ca2+ or phorbolester-dependent potentiation of synaptic release.
Ruiter M, Houy S, Engholm-Keller K, Graham ME, Sørensen JB
Molecular and cellular neurosciences (2019) : 103452. 132 011 WB; tested species: mouse

Development of lentiviral vectors for efficient glutamatergic-selective gene expression in cultured hippocampal neurons.
Egashira Y, Mori Y, Yanagawa Y, Takamori S
Scientific reports (2018) 81: 15156. 132 011 ICC; tested species: mouse

STAC3 stably interacts through its C1 domain with CaV1.1 in skeletal muscle triads.
Campiglio M, Flucher BE
Scientific reports (2017) 7: 41003. 132 011 ICC

CNS-localized myeloid cells capture living invading T cells during neuroinflammation.
Wasser B, Luchtman D, Löffel J, Robohm K, Birkner K, Stroh A, Vogelaar CF, Zipp F, Bittner S
The Journal of experimental medicine (2020) 2176: . 132 011 IHC; tested species: mouse

Cat. No.: 132 011

Amount: 100 µg

Price: $415.00

STAC3 stably interacts through its C1 domain with CaV1.1 in skeletal muscle triads.
Campiglio M, Flucher BE
Scientific reports (2017) 7: 41003. 132 011 ICC

ICC

IHC

Background

Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.

1 ) van Roessel P et al., Nat. Cell Biol. (2002)
2 ) Stephens DJ et al., J. Cell. Sci. (2001)
3 ) Chamberlain C et al., Traffic (2000)

Protocols

Standard protocol

ICC

IHC

Other products for this target

GFP antibody - 132 011

References for GFP - 132 011