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GFP - 132 011

A universal tool in cell biology
Monoclonal mouse purified IgG
Cat. No.: 132 011
Amount: 100 µg
Price: 415.00 €
Cat. No. 132 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not recommended
IP: yes
ICC: 1 : 100 gallery  
IHC: 1 : 100 gallery  
IHC-P/FFPE: not tested yet
Clone 270F3
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 238 from GFP (UniProt Id: P42212)
Epitop Epitop: AA 183 to 188 from GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks Cat. no. 132 111 or 132 002 is recommended for WB.
Data sheet 132_011.pdf

References for GFP - 132 011

SNAP-25 phosphorylation at Ser187 is not involved in Ca2+ or phorbolester-dependent potentiation of synaptic release.
Ruiter M, Houy S, Engholm-Keller K, Graham ME, Sørensen JB
Molecular and cellular neurosciences (2019) : 103452. 132 011 WB; tested species: mouse
Development of lentiviral vectors for efficient glutamatergic-selective gene expression in cultured hippocampal neurons.
Egashira Y, Mori Y, Yanagawa Y, Takamori S
Scientific reports (2018) 81: 15156. 132 011 ICC; tested species: mouse
STAC3 stably interacts through its C1 domain with CaV1.1 in skeletal muscle triads.
Campiglio M, Flucher BE
Scientific reports (2017) 7: 41003. 132 011 ICC
Cat. No.: 132 011
Quantity: 100 µg
Price: 415.00 €
SNAP-25 phosphorylation at Ser187 is not involved in Ca2+ or phorbolester-dependent potentiation of synaptic release.
Ruiter M, Houy S, Engholm-Keller K, Graham ME, Sørensen JB
Molecular and cellular neurosciences (2019) : 103452. 132 011 WB; tested species: mouse
Development of lentiviral vectors for efficient glutamatergic-selective gene expression in cultured hippocampal neurons.
Egashira Y, Mori Y, Yanagawa Y, Takamori S
Scientific reports (2018) 81: 15156. 132 011 ICC; tested species: mouse
STAC3 stably interacts through its C1 domain with CaV1.1 in skeletal muscle triads.
Campiglio M, Flucher BE
Scientific reports (2017) 7: 41003. 132 011 ICC
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.