Home|All ProductsNew ProductsPrimary AntibodiesSecondary AntibodiesFluoTagsControl ProteinsTissue lysatesChemicalsmCLINGAffinity ResinsSampler KitsKits|OrderingShippingPaymentRecommended DistributorsTerms & ConditionsPrivacy Policy|ContactTechnical SupportMeet us live|Antibody DevelopmentSingle-Domain Antibody DevelopmentPeptide Design and SynthesisCloning and Protein ExpressionProduction and PurificationAntibody LabelingReferencespAbmAbs|Protocol for Western blots: AP stainingProtocol for Western blots: ECL detectionProtocol for Western blots: Fluorescent detectionProtocol for antibody preadsorptionProtocol for ICC: PFA fixationProtocol for ICC: Methanol fixationProtocol for IHCProtocol for IHC-P: chromogenic detectionProtocol for IHC-P: fluorescent detectionProtocols for IHC and IHC-P tissue preparationProtocols for antigen retrievalProtocol for ICC: mCling stainingProtocol for IHC: mCling stainingProtocol for IPProtocol for IP: Selector ResinsProtocol for IP: m6AProtocol for IP: m6A sequencingProtocol for IP: m3G capProtocol for ALFA Selector ResinsProtocol for extraction of membrane proteins with MemEx kitStandard protocol for sandwich ELISAProtocol for sandwich ELISA of membrane proteinsProtocol for antibody purificationProtocol for preparation of P2, LP1 and LP2 fractions|Antibody ValidationFeatured TopicsFeatured ProductsNewsFAQProtocolsVideoDownloadsSySy PublicationspAbmAbs|Technical Support|The CompanyImpressum - Legal NoticePartners
All our antibodies are lyophilized from PBS. To reconstitute the antibody in PBS, add the amount of deionized water given in the respective datasheet. If higher volumes are preferred, add water as mentioned above and then the desired amount of PBS and a stabilizing carrier protein (e.g. BSA) to a final concentration of 2%. Some of our antibodies already contain albumin. Take this into account when adding more carrier protein.
For complete reconstitution, carefully remove the lid. After adding water, briefly vortex the solution. You can spin down the liquid by placing the vial into a 50 ml centrifugation tube filled with paper.
If desired, add small amounts of azide or thimerosal to prevent microbial growth. This is especially recommended if you want to keep an aliquot a 4°C.
After reconstitution of fluorescence-labeled antibodies, add 1 : 1 (v/v) glycerol to a final concentration of 50%. This lowers the freezing point of your stock and keeps your antibody in liquid state at -20°C.
Glycerol may also be added to unlabeled primary antibodies. It is a suitable way to avoid freeze-thaw cycles.
