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Home > Products > 131 005
- will be discontinued soon
Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received Guinea pig polyclonal VGAT antibody 131 005. It will be replaced by our new Guinea pig monoclonal recombinant IgG VGAT antibody 131 308.

VGAT antibody cytoplasmic domain - 131 005

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Guinea pig polyclonal purified antibody
Cat. No.: 131 005
Amount: 50 µg
Price: $480.00
Cat. No. 131 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: rat (O35458), mouse (O35633).
Other species not tested yet.
Matching control protein/peptide 131-0GP
Remarks

WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
IHC: Signal intensity can be enhanced by antigen retrieval at pH6 prior to blocking step.

Data sheet 131_005.pdf

References for VGAT - 131 005

Autism risk gene Cul3 alters neuronal morphology via caspase-3 activity in mouse hippocampal neurons.
Xia QQ, Singh A, Wang J, Xuan ZX, Singer JD, Powell CM
Frontiers in cellular neuroscience (2024) 18: 1320784. 131 005 ICC; tested species: mouse
Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2.
Boshans LL, Soh H, Wood WM, Nolan TM, Mandoiu II, Yanagawa Y, Tzingounis AV, Nishiyama A
Scientific reports (2021) 111: 3552. 131 005 ICC; tested species: mouse
Neuroligin-4 Regulates Excitatory Synaptic Transmission in Human Neurons.
Marro SG, Chanda S, Yang N, Janas JA, Valperga G, Trotter J, Zhou B, Merrill S, Yousif I, Shelby H, Vogel H, et al.
Neuron (2019) 1034: 617-626.e6. 131 005 ICC; tested species: human
Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8151-63. 131 005 ICC; tested species: rat
GABAergic-like dopamine synapses in the brain.
Kim HJ, Hwang B, Reva M, Lee J, Lee BE, Lee Y, Cho EJ, Jeong M, Lee SE, Myung K, Baik JH, et al.
Cell reports (2023) 4210: 113239. 131 005 IHC; tested species: mouse
Engram cell connectivity as a mechanism for information encoding and memory function.
Ortega-de San Luis C, Pezzoli M, Urrieta E, Ryan TJ
Current biology : CB (2023) 3324: 5368-5380.e5. 131 005 IHC; tested species: mouse
Molecular self-avoidance in synaptic neurexin complexes.
Wang CY, Trotter JH, Liakath-Ali K, Lee SJ, Liu X, Südhof TC
Science advances (2021) 751: eabk1924. 131 005 IHC; tested species: mouse
Deleting Mecp2 from the cerebellum rather than its neuronal subtypes causes a delay in motor learning in mice.
Achilly NP, He LJ, Kim OA, Ohmae S, Wojaczynski GJ, Lin T, Sillitoe RV, Medina JF, Zoghbi HY
eLife (2021) 10: . 131 005 IHC; tested species: mouse
Corelease of dopamine and GABA by a retinal dopaminergic neuron.
Hirasawa H, Betensky RA, Raviola E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3238: 13281-91. 131 005 IHC; tested species: mouse
Cat. No.: 131 005
Amount: 50 µg
Price: $480.00
Autism risk gene Cul3 alters neuronal morphology via caspase-3 activity in mouse hippocampal neurons.
Xia QQ, Singh A, Wang J, Xuan ZX, Singer JD, Powell CM
Frontiers in cellular neuroscience (2024) 18: 1320784. 131 005 ICC; tested species: mouse
Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2.
Boshans LL, Soh H, Wood WM, Nolan TM, Mandoiu II, Yanagawa Y, Tzingounis AV, Nishiyama A
Scientific reports (2021) 111: 3552. 131 005 ICC; tested species: mouse
Neuroligin-4 Regulates Excitatory Synaptic Transmission in Human Neurons.
Marro SG, Chanda S, Yang N, Janas JA, Valperga G, Trotter J, Zhou B, Merrill S, Yousif I, Shelby H, Vogel H, et al.
Neuron (2019) 1034: 617-626.e6. 131 005 ICC; tested species: human
Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8151-63. 131 005 ICC; tested species: rat
GABAergic-like dopamine synapses in the brain.
Kim HJ, Hwang B, Reva M, Lee J, Lee BE, Lee Y, Cho EJ, Jeong M, Lee SE, Myung K, Baik JH, et al.
Cell reports (2023) 4210: 113239. 131 005 IHC; tested species: mouse
Engram cell connectivity as a mechanism for information encoding and memory function.
Ortega-de San Luis C, Pezzoli M, Urrieta E, Ryan TJ
Current biology : CB (2023) 3324: 5368-5380.e5. 131 005 IHC; tested species: mouse
Molecular self-avoidance in synaptic neurexin complexes.
Wang CY, Trotter JH, Liakath-Ali K, Lee SJ, Liu X, Südhof TC
Science advances (2021) 751: eabk1924. 131 005 IHC; tested species: mouse
Deleting Mecp2 from the cerebellum rather than its neuronal subtypes causes a delay in motor learning in mice.
Achilly NP, He LJ, Kim OA, Ohmae S, Wojaczynski GJ, Lin T, Sillitoe RV, Medina JF, Zoghbi HY
eLife (2021) 10: . 131 005 IHC; tested species: mouse
Corelease of dopamine and GABA by a retinal dopaminergic neuron.
Hirasawa H, Betensky RA, Raviola E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3238: 13281-91. 131 005 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC
IHC
IHC-P
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
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