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VGAT protein cytoplasmic domain - 131-0GP

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
control protein
Cat. No.: 131-0GP
Amount: 100 µg
Price: $90.00
Cat. No. 131-0GP 100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Immunogen Recombinant protein corresponding to AA 2 to 115 from rat VGAT (UniProt Id: O35458)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 131 004, 131 006, 131 013, 131 005
Remarks

This control protein consists of the Strep-Tag® fusion protein (aa 2 - 115) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 131-0gp.pdf
Cat. No.: 131-0GP
Quantity: 100 µg
Price: $90.00
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
Protocols