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Home > Products > 131 006

VGAT antibody cytoplasmic domain - 131 006

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Chicken polyclonal purified antibody
Cat. No.: 131 006
Amount: 50 µg
Price: $385.00
Cat. No. 131 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
IHC-Fr: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: rat (O35458), mouse (O35633), human (Q9H598).
Other species not tested yet.
Matching control protein/peptide 131-0GP
Remarks

IHC-Fr: PFA fixation is recommended.
Data sheet 131_006.pdf

References for VGAT - 131 006

Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.
Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K
Cells (2021) 1011: . 131 006 ICC; tested species: rat
Increased number of excitatory synapsis and decreased number of inhibitory synapsis in the prefrontal cortex in autism.
Vakilzadeh G, Maseko BC, Bartely TD, McLennan YA, Martínez-Cerdeño V
Cerebral cortex (New York, N.Y. : 1991) (2024) 3413: 121-128. 131 006 IHC; tested species: human
Shank2/3 double knockout-based screening of cortical subregions links the retrosplenial area to the loss of social memory in autism spectrum disorders.
Garrido D, Beretta S, Grabrucker S, Bauer HF, Bayer D, Sala C, Verpelli C, Roselli F, Bockmann J, Proepper C, Catanese A, et al.
Molecular psychiatry (2022) : . 131 006 IHC; tested species: mouse
Social Play Behavior Is Critical for the Development of Prefrontal Inhibitory Synapses and Cognitive Flexibility in Rats.
Bijlsma A, Omrani A, Spoelder M, Verharen JPH, Bauer L, Cornelis C, de Zwart B, van Dorland R, Vanderschuren LJMJ, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4246: 8716-8728. 131 006 IHC; tested species: rat
Paradoxical network excitation by glutamate release from VGluT3+ GABAergic interneurons.
Pelkey KA, Calvigioni D, Fang C, Vargish G, Ekins T, Auville K, Wester JC, Lai M, Mackenzie-Gray Scott C, Yuan X, Hunt S, et al.
eLife (2020) 9: . 131 006 IHC; tested species: mouse
The Presynaptic Protein Mover Is Differentially Expressed Across Brain Areas and Synapse Types.
Wallrafen R, Dresbach T
Frontiers in neuroanatomy (2018) 12: 58. 131 006 IHC; tested species: mouse
Enhanced GABAergic Immunoreactivity in Hippocampal Neurons and Astroglia of Multiple Sclerosis Patients.
Kiljan S, Prins M, Baselmans BM, Bol JGJM, Schenk GJ, van Dam AM
Journal of neuropathology and experimental neurology (2019) 786: 480-491. 131 006 IHC-P; tested species: human
Vitamin B6 Deficiency Induces Autism-Like Behaviors in Rats by Regulating mTOR-Mediated Autophagy in the Hippocampus.
Chen L, Li J, Liu X, Zhao Z, Jin Y, Fu Y, Zhou A, Wang C, Zhou Y
Behavioural neurology (2023) 2023: 6991826. 131 006 IHC-Fr; tested species: rat
Cat. No.: 131 006
Amount: 50 µg
Price: $385.00
Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.
Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K
Cells (2021) 1011: . 131 006 ICC; tested species: rat
Increased number of excitatory synapsis and decreased number of inhibitory synapsis in the prefrontal cortex in autism.
Vakilzadeh G, Maseko BC, Bartely TD, McLennan YA, Martínez-Cerdeño V
Cerebral cortex (New York, N.Y. : 1991) (2024) 3413: 121-128. 131 006 IHC; tested species: human
Shank2/3 double knockout-based screening of cortical subregions links the retrosplenial area to the loss of social memory in autism spectrum disorders.
Garrido D, Beretta S, Grabrucker S, Bauer HF, Bayer D, Sala C, Verpelli C, Roselli F, Bockmann J, Proepper C, Catanese A, et al.
Molecular psychiatry (2022) : . 131 006 IHC; tested species: mouse
Social Play Behavior Is Critical for the Development of Prefrontal Inhibitory Synapses and Cognitive Flexibility in Rats.
Bijlsma A, Omrani A, Spoelder M, Verharen JPH, Bauer L, Cornelis C, de Zwart B, van Dorland R, Vanderschuren LJMJ, Wierenga CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4246: 8716-8728. 131 006 IHC; tested species: rat
Paradoxical network excitation by glutamate release from VGluT3+ GABAergic interneurons.
Pelkey KA, Calvigioni D, Fang C, Vargish G, Ekins T, Auville K, Wester JC, Lai M, Mackenzie-Gray Scott C, Yuan X, Hunt S, et al.
eLife (2020) 9: . 131 006 IHC; tested species: mouse
The Presynaptic Protein Mover Is Differentially Expressed Across Brain Areas and Synapse Types.
Wallrafen R, Dresbach T
Frontiers in neuroanatomy (2018) 12: 58. 131 006 IHC; tested species: mouse
Enhanced GABAergic Immunoreactivity in Hippocampal Neurons and Astroglia of Multiple Sclerosis Patients.
Kiljan S, Prins M, Baselmans BM, Bol JGJM, Schenk GJ, van Dam AM
Journal of neuropathology and experimental neurology (2019) 786: 480-491. 131 006 IHC-P; tested species: human
Vitamin B6 Deficiency Induces Autism-Like Behaviors in Rats by Regulating mTOR-Mediated Autophagy in the Hippocampus.
Chen L, Li J, Liu X, Zhao Z, Jin Y, Fu Y, Zhou A, Wang C, Zhou Y
Behavioural neurology (2023) 2023: 6991826. 131 006 IHC-Fr; tested species: rat
ICC
IHC
IHC-P
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
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