Tailor-made Antibodies
and Tools for Life Science

VGAT antibody cytoplasmic domain - 131 013 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 131 013
Amount: 50 µg
Price: $380.00
Cat. No. 131 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
EM: yes This antibody is first choice for electron microscopy.
Immunogen Recombinant protein corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: rat (O35458), mouse (O35633), zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 36073542
Matching control protein/peptide 131-0GP
Remarks

WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Data sheet 131_013.pdf

References for VGAT - 131 013

De Novo Missense Variants in SLC32A1 Cause a Developmental and Epileptic Encephalopathy Due to Impaired GABAergic Neurotransmission.
Platzer K, Sticht H, Bupp C, Ganapathi M, Pereira EM, Le Guyader G, Bilan F, Henderson LB, Lemke JR, Taschenberger H, Brose N, et al.
Annals of neurology (2022) : . 131 013 WB; KO verified; tested species: mouse
Neuroligin-3 Regulates Excitatory Synaptic Transmission and EPSP-Spike Coupling in the Dentate Gyrus In Vivo.
Muellerleile J, Vnencak M, Ippolito A, Krueger-Burg D, Jungenitz T, Schwarzacher SW, Jedlicka P
Molecular neurobiology (2021) : . 131 013 WB; tested species: mouse
Selective disruption of inhibitory synapses leading to neuronal hyperexcitability at an early stage of tau pathogenesis in a mouse model.
Shimojo M, Takuwa H, Takado Y, Tokunaga M, Tsukamoto S, Minatohara K, Ono M, Seki C, Maeda J, Urushihata T, Minamihisamatsu T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 131 013 WB, IHC; tested species: mouse
Redundant and nonredundant organismal functions of EPS15 and EPS15L1.
Milesi C, Alberici P, Pozzi B, Oldani A, Beznoussenko GV, Raimondi A, Soppo BE, Amodio S, Caldieri G, Malabarba MG, Bertalot G, et al.
Life science alliance (2019) 21: . 131 013 WB; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 131 013 WB; tested species: mouse
Cat. No.: 131 013
Amount: 50 µg
Price: $380.00