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VGAT antibody cytoplasmic domain - 131 005

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Polyclonal Guinea pig purified antibody
Cat. No.: 131 005
Amount: 50 µg
Price: $460.00
Cat. No. 131 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 2 to 115 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity Specific for VGAT
Matching control protein/peptide 131-0GP
Remarks

VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
IHC: Signal intensity can be enhanced by antigen retrieval at pH6 prior to blocking step.

Data sheet 131_005.pdf

References for VGAT - 131 005

Neuroligin-4 Regulates Excitatory Synaptic Transmission in Human Neurons.
Marro SG, Chanda S, Yang N, Janas JA, Valperga G, Trotter J, Zhou B, Merrill S, Yousif I, Shelby H, Vogel H, et al.
Neuron (2019) 1034: 617-626.e6. 131 005 ICC; tested species: human
Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8151-63. 131 005 ICC; tested species: rat
Deleting Mecp2 from the cerebellum rather than its neuronal subtypes causes a delay in motor learning in mice.
Achilly NP, He LJ, Kim OA, Ohmae S, Wojaczynski GJ, Lin T, Sillitoe RV, Medina JF, Zoghbi HY
eLife (2021) 10: . 131 005 IHC; tested species: mouse
Corelease of dopamine and GABA by a retinal dopaminergic neuron.
Hirasawa H, Betensky RA, Raviola E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3238: 13281-91. 131 005 IHC; tested species: mouse
Cat. No.: 131 005
Quantity: 50 µg
Price: $460.00
Neuroligin-4 Regulates Excitatory Synaptic Transmission in Human Neurons.
Marro SG, Chanda S, Yang N, Janas JA, Valperga G, Trotter J, Zhou B, Merrill S, Yousif I, Shelby H, Vogel H, et al.
Neuron (2019) 1034: 617-626.e6. 131 005 ICC; tested species: human
Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8151-63. 131 005 ICC; tested species: rat
Deleting Mecp2 from the cerebellum rather than its neuronal subtypes causes a delay in motor learning in mice.
Achilly NP, He LJ, Kim OA, Ohmae S, Wojaczynski GJ, Lin T, Sillitoe RV, Medina JF, Zoghbi HY
eLife (2021) 10: . 131 005 IHC; tested species: mouse
Corelease of dopamine and GABA by a retinal dopaminergic neuron.
Hirasawa H, Betensky RA, Raviola E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3238: 13281-91. 131 005 IHC; tested species: mouse
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.