Synaptic Systems

Cat. No.: 131 005

Amount: 50 µg

Price: $475.00

Cat. No. 131 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (see remarks) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity	Reacts with: rat (O35458), mouse (O35633). Other species not tested yet.
Matching control protein/peptide	131-0GP
Remarks	WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. IHC: Signal intensity can be enhanced by antigen retrieval at pH6 prior to blocking step.
Data sheet	131_005.pdf

References for VGAT - 131 005

Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2.
Boshans LL, Soh H, Wood WM, Nolan TM, Mandoiu II, Yanagawa Y, Tzingounis AV, Nishiyama A
Scientific reports (2021) 111: 3552. 131 005 ICC; tested species: mouse

Neuroligin-4 Regulates Excitatory Synaptic Transmission in Human Neurons.
Marro SG, Chanda S, Yang N, Janas JA, Valperga G, Trotter J, Zhou B, Merrill S, Yousif I, Shelby H, Vogel H, et al.
Neuron (2019) 1034: 617-626.e6. 131 005 ICC; tested species: human

Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8151-63. 131 005 ICC; tested species: rat

GABAergic-like dopamine synapses in the brain.
Kim HJ, Hwang B, Reva M, Lee J, Lee BE, Lee Y, Cho EJ, Jeong M, Lee SE, Myung K, Baik JH, et al.
Cell reports (2023) 4210: 113239. 131 005 IHC; tested species: mouse

Engram cell connectivity as a mechanism for information encoding and memory function.
Ortega-de San Luis C, Pezzoli M, Urrieta E, Ryan TJ
Current biology : CB (2023) 3324: 5368-5380.e5. 131 005 IHC; tested species: mouse

Molecular self-avoidance in synaptic neurexin complexes.
Wang CY, Trotter JH, Liakath-Ali K, Lee SJ, Liu X, Südhof TC
Science advances (2021) 751: eabk1924. 131 005 IHC; tested species: mouse

Deleting Mecp2 from the cerebellum rather than its neuronal subtypes causes a delay in motor learning in mice.
Achilly NP, He LJ, Kim OA, Ohmae S, Wojaczynski GJ, Lin T, Sillitoe RV, Medina JF, Zoghbi HY
eLife (2021) 10: . 131 005 IHC; tested species: mouse

Corelease of dopamine and GABA by a retinal dopaminergic neuron.
Hirasawa H, Betensky RA, Raviola E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3238: 13281-91. 131 005 IHC; tested species: mouse

Cat. No.: 131 005

Amount: 50 µg

Price: $475.00

Molecular self-avoidance in synaptic neurexin complexes.
Wang CY, Trotter JH, Liakath-Ali K, Lee SJ, Liu X, Südhof TC
Science advances (2021) 751: eabk1924. 131 005 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.

1 ) Chaudhry FA et al., J. Neurosci. (1998)
2 ) McIntire SL et al., Nature (1997)
3 ) Sagné C et al., FEBS Lett. (1997)
4 ) Hell JW et al., EMBO J. (1988)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

VGAT antibody cytoplasmic domain - 131 005

References for VGAT - 131 005