Tailor-made Antibodies
and Tools for Life Science
- will be discontinued soon
Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received Guinea pig polyclonal VGAT antibody 131 004. It will be replaced by our new Guinea pig monoclonal recombinant IgG VGAT antibody 131 308.

VGAT antibody cytoplasmic domain - 131 004 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Guinea pig polyclonal antiserum
Cat. No.: 131 004
Amount: 100 µl
Price: $405.00
Cat. No. 131 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: yes gallery  
IHC-Fr: 1 : 500 (see remarks)
ExM: 1 : 250 (see remarks) gallery  
EM: yes
Immunogen Recombinant protein corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: rat (O35458), mouse (O35633), zebrafish, ape.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 131-0GP
Remarks

WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
IHC-Fr: 5 min MeOH and PFA fixation are possible.
ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)

Data sheet 131_004.pdf

References for VGAT - 131 004

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 131 004 WB, ICC; tested species: mouse
Loss of mitochondrial enzyme GPT2 leads to reprogramming of synaptic glutamate metabolism.
Baytas O, Davidson SM, Kauer JA, Morrow EM
Molecular brain (2024) 171: 87. 131 004 WB; tested species: mouse
Spectrin-beta 2 facilitates the selective accumulation of GABAA receptors at somatodendritic synapses.
Smalley JL, Cho N, Ng SFJ, Choi C, Lemons AHS, Chaudry S, Bope CE, Dengler JS, Zhang C, Rasband MN, Davies PA, et al.
Communications biology (2023) 61: 11. 131 004 WB, ICC; tested species: mouse
Microglia enable cross-modal plasticity by removing inhibitory synapses.
Hashimoto A, Kawamura N, Tarusawa E, Takeda I, Aoyama Y, Ohno N, Inoue M, Kagamiuchi M, Kato D, Matsumoto M, Hasegawa Y, et al.
Cell reports (2023) 425: 112383. 131 004 WB, IHC; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 131 004 WB, UPTAKE; tested species: rat
Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses.
López-Hernández T, Takenaka KI, Mori Y, Kongpracha P, Nagamori S, Haucke V, Takamori S
eLife (2022) 11: . 131 004 WB; tested species: mouse
Brevican, Neurocan, Tenascin-C, and Tenascin-R Act as Important Regulators of the Interplay Between Perineuronal Nets, Synaptic Integrity, Inhibitory Interneurons, and Otx2.
Mueller-Buehl C, Reinhard J, Roll L, Bader V, Winklhofer KF, Faissner A
Frontiers in cell and developmental biology (2022) 10: 886527. 131 004 WB, IHC; tested species: mouse
Systemic inflammation induced the delayed reduction of excitatory synapses in the CA3 during ageing.
Manabe T, Rácz I, Schwartz S, Oberle L, Santarelli F, Emmrich JV, Neher JJ, Heneka MT
Journal of neurochemistry (2021) 1593: 525-542. 131 004 WB, IHC; tested species: mouse
A proline-rich motif on VGLUT1 reduces synaptic vesicle super-pool and spontaneous release frequency.
Zhang XM, François U, Silm K, Angelo MF, Fernandez-Busch MV, Maged M, Martin C, Bernard V, Cordelières FP, Deshors M, Pons S, et al.
eLife (2019) 8: . 131 004 WB; tested species: mouse
HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q.
Hammond JW, Qiu WQ, Marker DF, Chamberlain JM, Greaves-Tunnell W, Bellizzi MJ, Lu SM, Gelbard HA
Glia (2018) : . 131 004 WB; tested species: mouse
Expression of Neurofilament Subunits at Neocortical Glutamatergic and GABAergic Synapses.
Bragina L, Conti F
Frontiers in neuroanatomy (2018) 12: 74. 131 004 WB, IHC; tested species: rat
Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation.
Simões AP, Silva CG, Marques JM, Pochmann D, Porciúncula LO, Ferreira S, Oses JP, Beleza RO, Real JI, Köfalvi A, Bahr BA, et al.
Cell death & disease (2018) 93: 297. 131 004 WB; tested species: rat
Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.
Yakoub AM, Sadek M
Cell transplantation (2018) 273: 393-406. 131 004 WB; tested species: human
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 131 004 WB; tested species: mouse
Cellular plasticity induced by anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies.
Peng X, Hughes EG, Moscato EH, Parsons TD, Dalmau J, Balice-Gordon RJ
Annals of neurology (2015) 773: 381-98. 131 004 WB
Development of dissociated cryopreserved rat cortical neurons in vitro.
Schock SC, Jolin-Dahel KS, Schock PC, Theiss S, Arbuthnott GW, Garcia-Munoz M, Staines WA
Journal of neuroscience methods (2012) 2052: 324-33. 131 004 WB, IHC
Cat. No.: 131 004
Amount: 100 µl
Price: $405.00