VGAT protein cytoplasmic domain - 131-0GP

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles

control protein

Cat. No.: 131-0GP

Amount: 100 µg

Price: $105.00

Cat. No. 131-0GP	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to residues near the amino terminus of rat VGAT (UniProt Id: O35458)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	131 004, 131 006, 131 013, 131 005
Remarks	This control protein consists of the Strep-Tag^® fusion protein (aa 2 - 115) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	131-0gp.pdf

Cat. No.: 131-0GP

Amount: 100 µg

Price: $105.00

Background

The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.

1 ) Chaudhry FA et al., J. Neurosci. (1998)
2 ) McIntire SL et al., Nature (1997)
3 ) Sagné C et al., FEBS Lett. (1997)
4 ) Hell JW et al., EMBO J. (1988)

Protocols

OTHER

Preadsorbtion

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