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Home > Products > 110 403

Syntaxin1B antibody - 110 403 K.O.

Syntaxin1 is a plasma membrane SNARE protein essential for synaptic vesicle fusion
Rabbit polyclonal purified antibody
Cat. No.: 110 403
Amount: 50 µg
Price: $460.00
Cat. No. 110 403 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 171 to 187 from rat Syntaxin1B (UniProt Id: P61265)
Reactivity Reacts with: human (P61266), rat (P61265), mouse (P61264), hamster, cow, pig, chicken, zebrafish.
Other species not tested yet.
Specificity Specific for syntaxin 1B, no cross reactivity to syntaxin 1A. K.O. validated PubMed: 32572454
Matching control protein/peptide 110-1BP
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.
Data sheet 110_403.pdf

References for Syntaxin1B - 110 403

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 110 403 WB; tested species: mouse
Epilepsy-causing STX1B mutations translate altered protein functions into distinct phenotypes in mouse neurons.
Vardar G, Gerth F, Schmitt XJ, Rautenstrauch P, Trimbuch T, Schubert J, Lerche H, Rosenmund C, Freund C
Brain : a journal of neurology (2020) : . 110 403 WB, ICC; KO verified; tested species: mouse
Changes in synaptic proteins precede neurodegeneration markers in preclinical Alzheimer's disease cerebrospinal fluid.
Lleó A, Núñez-Llaves R, Alcolea D, Chiva C, Balateu-Paños D, Colom-Cadena M, Gomez-Giro G, Muñoz L, Querol-Vilaseca M, Pegueroles J, Rami L, et al.
Molecular & cellular proteomics : MCP (2019) : . 110 403 WB; tested species: human
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 110 403 WB
Titration of Syntaxin1 in mammalian synapses reveals multiple roles in vesicle docking, priming, and release probability.
Arancillo M, Min SW, Gerber S, Münster-Wandowski A, Wu YJ, Herman M, Trimbuch T, Rah JC, Ahnert-Hilger G, Riedel D, Südhof TC, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16698-714. 110 403 WB, ICC
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 403 WB; tested species: mouse
Cat. No.: 110 403
Amount: 50 µg
Price: $460.00
WB
ICC
IHC
IHC-P
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